LOCUS       Exported                7764 bp ds-DNA     circular SYN 10-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..4
                     /label=GA_Insert3
     misc_feature    9..362
                     /label=GA Insert 4
     primer_bind     13..33
                     /label=V_InsertFor
     primer_bind     complement(110..126)
                     /label=V_insertREV
     primer_bind     complement(350..366)
                     /label=V_insertREV
     misc_feature    363..698
                     /label=GA Insert 5
     misc_feature    699..917
                     /label=Last Insert
     primer_bind     703..728
                     /label=Frag3Forward
     primer_bind     703..728
                     /label=Frag3Forwarda
     primer_bind     703..728
                     /label=Frag3Forward`b
     primer_bind     714..741
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     778..801
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     864..891
                     /label=Primer 1a
                     /note="FWD"
     CDS             918..939
                     /codon_start=1
                     /label=Expressed Protein
                     /translation="HHHHHH"
     misc_feature    918
                     /label=MCS
                     /note="multiple cloning site"
     CDS             919..936
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1003..1050
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1087..1542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1569..1673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1674..2534
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2350..2377)
                     /label=Primer 2
     primer_bind     2378..2400
                     /label=Primer 1
                     /note="Mutagenesis Primer"
     primer_bind     2378..2400
                     /label=Mutagenic Primer
     rep_origin      2705..3293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3723..3914)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4723..5805)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(5806..5883)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        6192..6210
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6211..6235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6266..6271
                     /note="ribosome binding site"
     CDS             6280
                     /codon_start=1
                     /label=ATG
                     /translation=""
     CDS             6280
                     /codon_start=1
                     /label=Expressed Protein
                     /translation=""
     misc_feature    6281..6540
                     /label=GA Insert 1
     primer_bind     6301..6326
                     /label=Frag3Forward
     primer_bind     6301..6326
                     /label=Frag3Forwarda
     primer_bind     6301..6326
                     /label=Frag3Forward`b
     primer_bind     6304..6323
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     6355..6368
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(6517..6544)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6517..6539)
                     /label=Frag2Rev
     primer_bind     complement(6517..6539)
                     /label=Frag2Reva
     primer_bind     complement(6517..6539)
                     /label=Frag2Rev`b
     primer_bind     complement(6532..6545)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6532..6545)
                     /label=Frag2Rev
     primer_bind     complement(6532..6545)
                     /label=Frag2Reva
     primer_bind     complement(6532..6545)
                     /label=Frag2Rev`b
     primer_bind     6541..6566
                     /label=Frag3Forward
     primer_bind     6541..6566
                     /label=Frag3Forwarda
     primer_bind     6541..6566
                     /label=Frag3Forward`b
     primer_bind     6541..6563
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    6544..6777
                     /label=GA Insert 1
     primer_bind     6595..6608
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(6757..6781)
                     /label=Frag2Rev
     primer_bind     complement(6757..6781)
                     /label=Frag2Reva
     primer_bind     complement(6757..6781)
                     /label=Frag2Rev`b
     primer_bind     complement(6757..6779)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     6778..6806
                     /label=Frag3Forwarda
     primer_bind     6778..6806
                     /label=Frag3Forward`b
     misc_feature    6781..7103
                     /label=GA Insert 1
     primer_bind     6781..6806
                     /label=Frag3Forward
     primer_bind     6784..6803
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     6835..6848
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(6997..7022)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6997..7019)
                     /label=Frag2Rev
     primer_bind     complement(6997..7019)
                     /label=Frag2Reva
     primer_bind     complement(6997..7019)
                     /label=Frag2Rev`b
     primer_bind     7054..7071
                     /label=V_InsertFor
     primer_bind     complement(7094..7107)
                     /label=Frag3Reverse
     misc_feature    7104..7448
                     /label=GA Insert 2
     primer_bind     complement(7162..7181)
                     /label=Frag2Rev
     primer_bind     complement(7162..7181)
                     /label=Frag2Reva
     primer_bind     complement(7162..7181)
                     /label=Frag2Rev`b
     primer_bind     complement(7177..7204)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7177..7199)
                     /label=Frag2Rev
     primer_bind     complement(7177..7199)
                     /label=Frag2Reva
     primer_bind     complement(7177..7199)
                     /label=Frag2Rev`b
     primer_bind     complement(7192..7219)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7192..7205)
                     /label=Frag2Rev
     primer_bind     complement(7192..7205)
                     /label=Frag2Reva
     primer_bind     complement(7192..7205)
                     /label=Frag2Rev`b
     primer_bind     7264..7283
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    7449..7742
                     /label=GA_Insert3
     primer_bind     7600..7613
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     7602..7631
                     /label=Frag3Forwarda
     primer_bind     7602..7631
                     /label=Frag3Forward`b
     primer_bind     7606..7631
                     /label=Frag3Forward
     primer_bind     7659..7676
                     /label=Frag3Forward
     primer_bind     7659..7676
                     /label=Frag3Forwarda
     primer_bind     7659..7676
                     /label=Frag3Forward`b
     primer_bind     complement(7672..7694)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7672..7694)
                     /label=Frag2Rev
     primer_bind     complement(7672..7694)
                     /label=Frag2Reva
     primer_bind     complement(7672..7694)
                     /label=Frag2Rev`b
     primer_bind     complement(7702..7720)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7702..7720)
                     /label=Frag2Rev
     primer_bind     complement(7702..7720)
                     /label=Frag2Reva
     primer_bind     complement(7702..7720)
                     /label=Frag2Rev`b
ORIGIN
        1 ggcggctccg tgccaggcgt cggtgtgcct ggagtcgggg tgccaggggt aggagtccct
       61 ggcgtaggag tgccaggagt gggtgttccg ggtgtaggag ttcctggagt gggggttccc
      121 ggtgttggcg taccgggcgt tggagtacct ggtgtaggtg tgcccggtgt aggcgtgcct
      181 ggcgttggtg ttccaggagt tggggtcccc ggagttggcg ttccaggtgt cggggttccg
      241 ggggttggag tgcctggtgt gggcgtacct ggagtaggag tacccggtgt gggagtgccg
      301 ggagttggag tcccaggggt cggcgttcct ggggtgggag tcccgggggt gggcgttccc
      361 ggagttgggg tgcccggagt aggcgtacca ggcgtgggag taccgggtgt gggggtccca
      421 ggtgtagggg tacccggcgt gggtgtgccg ggtgtcggag ttcccggagt cggagtcccc
      481 ggggtgggtg taccgggggt cggggtacca ggggtggggg tgccgggggt aggcgttccg
      541 ggcgtagggg ttcctggtgt cggtgttccc ggcgtaggtg ttcctggcgt gggggtaccg
      601 ggagtgggcg tcccgggtgt tggagttccg ggagtaggtg tacccggagt gggagttcca
      661 ggcgttgggg ttccaggggt tggcgtccca ggagtaggtg tacctggggt aggtgtccct
      721 ggtgttggtg tacctggcgt cggagtgccc ggggttgggg tgcctggggt tggtgtgcca
      781 ggtgttgggg tacctggggt cggagtacca ggagtcggtg taccaggtgt gggtgtccca
      841 ggcgtaggcg tccctggagt tggtgtcccc ggtgtcggcg tacccggggt cggtgtcccg
      901 ggagtcggct ggctcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
      961 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1021 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     1081 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1141 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1201 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1261 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1321 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1381 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1441 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1501 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg
     1561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     2221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgcggta
     2401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2821 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3361 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3421 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     3541 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     3601 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3661 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3721 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     3781 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     3841 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     3901 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     3961 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     4021 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     4081 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     4141 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     4201 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     4261 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     4321 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     4381 ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca
     4441 gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc
     4501 atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc
     4561 tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc
     4621 cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc
     4681 ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag
     4741 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4801 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
     4861 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
     4921 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt
     4981 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
     5041 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
     5101 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
     5161 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
     5221 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
     5281 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
     5341 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
     5401 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
     5461 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
     5521 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
     5581 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
     5641 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
     5701 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
     5761 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     5821 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     5881 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     5941 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     6001 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     6061 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     6121 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
     6181 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     6241 agaaataatt ttgtttaact ttaagaagga gatatacata tgggtggttg gggaccagtg
     6301 cctggcgtcg gtgtacctgg tgctggtgta cccggtgctg gagtaccggg ggcaggtgta
     6361 ccaggtgcag gggtcccagg agctggagtt ccaggtgctg gggttcctgg tgcaggtgtt
     6421 cctggagcag gtgttcctgg tggcggagtt cctggcggtg gggtgcctgg tgggggcgtg
     6481 ccaggcgggg gcgttcccgg tggcggtgtc cccggtggtg gggttccagg gggtggggta
     6541 ccaggcgtcg gtgtacctgg tgctggtgta cccggtgctg gagtaccggg ggcaggtgta
     6601 ccaggtgcag gggtcccagg agctggagtt ccaggtgctg gggttcctgg tgcaggtgtt
     6661 cctggagcag gtgttcctgg tggcggagtt cctggcggtg gggtgcctgg tgggggcgtg
     6721 ccaggcgggg gcgttcccgg tggcggtgtc cccggtggtg gggttccagg gggtggggtc
     6781 cctggcgtcg gtgtacctgg tgctggtgta cccggtgctg gagtaccggg ggcaggtgta
     6841 ccaggtgcag gggtcccagg agctggagtt ccaggtgctg gggttcctgg tgcaggtgtt
     6901 cctggagcag gtgttcctgg tggcggagtt cctggcggtg gggtgcctgg tgggggcgtg
     6961 ccaggcgggg gcgttcccgg tggcggtgtc cccggtggtg gggttccagg gggtggggta
     7021 ccgggagtgg gcgtcccagg tgcgggcgtt cccggcgcag gtgtgcctgg agcgggagta
     7081 ccaggagccg gcgtgccagg ggcgggtgta ccaggggccg gagtgccagg tgctggagta
     7141 ccgggcgctg gcgttcctgg tggtggcgtt ccgggtggtg gagttcccgg tggtggggtt
     7201 ccaggtgggg gagtaccagg cggtggagtc cctggcggcg gtgtaccagg tggtggtgtg
     7261 cctggcgtcg gtgtacccgg tgcaggggtt ccaggagcgg gagttcctgg tgcgggtgtg
     7321 ccgggggctg gtgtacctgg agcaggtgtt ccgggagctg gggtccctgg ggcaggagtc
     7381 ccaggcgcag gcgtcccagg gggtggagtg ccgggcgggg gtgtgccagg tggcggagta
     7441 cctgggggcg gagtaccagg cggtggtgtg ccgggcggcg gtgtaccggg tggcggggta
     7501 cccggtgtgg gagttccggg agctggcgtc ccgggtgcag gagtcccagg ggctggagtg
     7561 ccaggagcag gcgtaccagg ggcgggagtc cccggagctg gtgtccctgg tgccggggta
     7621 ccgggtgctg gcgtaccagg gggcggtgta ccaggtggcg gcgtacctgg tggtggagtt
     7681 ccaggcggcg gggttcctgg gggtggtgtt ccaggtgggg gtgttcccgg cggcggttct
     7741 ggtggcggta ttgaaggccg cggt
//
