LOCUS       pKL2_160                9227 bp    DNA        circular     01-APR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      7913..8595
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6914..6921
                     /locus_tag="NotI"
                     /label="NotI"
                     /ApEinfo_label="NotI"
                     /ApEinfo_fwdcolor="#CC66FF"
                     /ApEinfo_revcolor="#CC66FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8677..8684
                     /locus_tag="NotI(1)"
                     /label="NotI(1)"
                     /ApEinfo_label="NotI"
                     /ApEinfo_fwdcolor="#CC66FF"
                     /ApEinfo_revcolor="#CC66FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3218..3238
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#7da8ff"
                     /ApEinfo_revcolor="#101ff8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1933..2166
                     /locus_tag="VP16"
                     /label="VP16"
                     /ApEinfo_label="VP16"
                     /ApEinfo_fwdcolor="#178bf8"
                     /ApEinfo_revcolor="#178bf8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3239..3472
                     /locus_tag="VP16(1)"
                     /label="VP16(1)"
                     /ApEinfo_label="VP16"
                     /ApEinfo_fwdcolor="#178bf8"
                     /ApEinfo_revcolor="#178bf8"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3149..3214
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#8fffb2"
                     /ApEinfo_revcolor="#17b631"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3476..4183
                     /locus_tag="mRuby2"
                     /label="mRuby2"
                     /ApEinfo_label="mRuby2"
                     /ApEinfo_fwdcolor="#fea391"
                     /ApEinfo_revcolor="#9e0c28"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    7913..8676
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#808080"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1006..1926
                     /locus_tag="Linker-ER (LBD)"
                     /label="Linker-ER (LBD)"
                     /ApEinfo_label="Linker-ER (LBD)"
                     /ApEinfo_fwdcolor="#99ff99"
                     /ApEinfo_revcolor="#99ff99"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        30..729
                     /locus_tag="pTEF1"
                     /label="pTEF1"
                     /ApEinfo_label="pTEF1"
                     /ApEinfo_fwdcolor="#0099ff"
                     /ApEinfo_revcolor="#0099ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4190..4453
                     /locus_tag="42-10-WT"
                     /label="42-10-WT"
                     /ApEinfo_label="42-10-WT"
                     /ApEinfo_fwdcolor="#9999CC"
                     /ApEinfo_revcolor="#9999CC"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      4503..4727
                     /locus_tag="tSSA1"
                     /label="tSSA1"
                     /ApEinfo_label="tSSA1"
                     /ApEinfo_fwdcolor="#ff99ff"
                     /ApEinfo_revcolor="#ff99ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2180..2404
                     /locus_tag="tENO2"
                     /label="tENO2"
                     /ApEinfo_label="tENO2"
                     /ApEinfo_fwdcolor="#ff99ff"
                     /ApEinfo_revcolor="#ff99ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             5155..6183
                     /locus_tag="HygromycinR"
                     /label="HygromycinR"
                     /ApEinfo_label="HygromycinR"
                     /ApEinfo_fwdcolor="#CCCCFF"
                     /ApEinfo_revcolor="#CCCCFF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        4776..5152
                     /locus_tag="AgTEF promoter"
                     /label="AgTEF promoter"
                     /ApEinfo_label="AgTEF promoter"
                     /ApEinfo_fwdcolor="#FFCCCC"
                     /ApEinfo_revcolor="#FFCCCC"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      6184..6385
                     /locus_tag="AgTEF terminator"
                     /label="AgTEF terminator"
                     /ApEinfo_label="AgTEF terminator"
                     /ApEinfo_fwdcolor="#FFCCCC"
                     /ApEinfo_revcolor="#FFCCCC"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6390..6909
                     /locus_tag="HO - 3' Homology"
                     /label="HO - 3' Homology"
                     /ApEinfo_label="HO - 3' Homology"
                     /ApEinfo_fwdcolor="#FFCC99"
                     /ApEinfo_revcolor="#FFCC99"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8689..9208
                     /locus_tag="HO - 5' Homology"
                     /label="HO - 5' Homology"
                     /ApEinfo_label="HO - 5' Homology"
                     /ApEinfo_fwdcolor="#FFCC99"
                     /ApEinfo_revcolor="#FFCC99"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4460..4471
                     /locus_tag="GS Linker"
                     /label="GS Linker"
                     /ApEinfo_label="GS Linker"
                     /ApEinfo_fwdcolor="#FF6666"
                     /ApEinfo_revcolor="#FF6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2667..2675
                     /locus_tag="Zif268 BS"
                     /label="Zif268 BS"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2677..2685
                     /locus_tag="Zif268 BS(1)"
                     /label="Zif268 BS(1)"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2687..2695
                     /locus_tag="Zif268 BS(2)"
                     /label="Zif268 BS(2)"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2697..2705
                     /locus_tag="Zif268 BS(3)"
                     /label="Zif268 BS(3)"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2707..2715
                     /locus_tag="Zif268 BS(4)"
                     /label="Zif268 BS(4)"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2717..2725
                     /locus_tag="Zif268 BS(5)"
                     /label="Zif268 BS(5)"
                     /ApEinfo_label="Zif268 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             739..999
                     /locus_tag="Zif268"
                     /label="Zif268"
                     /ApEinfo_label="Zif268"
                     /ApEinfo_fwdcolor="#9999CC"
                     /ApEinfo_revcolor="#9999CC"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2935..2945
                     /locus_tag="Mig1 BS"
                     /label="Mig1 BS"
                     /ApEinfo_label="Mig1 BS"
                     /ApEinfo_fwdcolor="#CC99FF"
                     /ApEinfo_revcolor="#CC99FF"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      6922..6948
                     /locus_tag="KanR Terminator"
                     /label="KanR Terminator"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#99FF33"
                     /ApEinfo_revcolor="#99FF33"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        7765..7912
                     /locus_tag="KanR Promoter"
                     /label="KanR Promoter"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#99FF33"
                     /ApEinfo_revcolor="#99FF33"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             6949..7764
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#339900"
                     /ApEinfo_revcolor="#339900"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5..25
                     /locus_tag="ConLS Scar"
                     /label="ConLS Scar"
                     /ApEinfo_label="ConLS Scar"
                     /ApEinfo_fwdcolor="#FFFF66"
                     /ApEinfo_revcolor="#FFFF66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2409..2429
                     /locus_tag="ConL1 Scar"
                     /label="ConL1 Scar"
                     /ApEinfo_label="ConL1 Scar"
                     /ApEinfo_fwdcolor="#FFFF66"
                     /ApEinfo_revcolor="#FFFF66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4732..4752
                     /locus_tag="ConRE' Scar"
                     /label="ConRE' Scar"
                     /ApEinfo_label="ConRE' Scar"
                     /ApEinfo_fwdcolor="#FFFF66"
                     /ApEinfo_revcolor="#FFFF66"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2434..2659
                     /locus_tag="pGal1-mut-5'"
                     /label="pGal1-mut-5'"
                     /ApEinfo_label="pGal1-mut-5'"
                     /ApEinfo_fwdcolor="#0099ff"
                     /ApEinfo_revcolor="#0099ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2732..3139
                     /locus_tag="pGal1-mut-3'"
                     /label="pGal1-mut-3'"
                     /ApEinfo_label="pGal1-mut-3'"
                     /ApEinfo_fwdcolor="#0099ff"
                     /ApEinfo_revcolor="#0099ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ctgaactggc cgataattgc agacgaacgc cttgccaaca gggagttctt cagagacatg
       61 gaggctcaaa acgaaattat tgacagccta gacatcaata gtcatacaac agaaagcgac
      121 cacccaactt tggctgataa tagcgtataa acaatgcata ctttgtacgt tcaaaataca
      181 atgcagtaga tatatttatg catattacat ataatacata tcacatagga agcaacaggc
      241 gcgttggact tttaattttc gaggaccgcg aatccttaca tcacacccaa tcccccacaa
      301 gtgatccccc acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt
      361 ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt
      421 tcccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa
      481 aaagacaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt
      541 ttctttttct tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat
      601 atttaagtta ataaacggtc atcaatttct caagtttcag tttcattttt cttgttctat
      661 tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt
      721 aattacaaaa gatctatggg tacccgccca tatgcttgcc ctgtcgagtc ctgcgatcgc
      781 cgcttttctc gctcggatga gcttacccgc catatccgca tccataccgg tcagaagccc
      841 ttccagtgtc gaatctgcat gcgtaacttc agtcgtagtg accaccttac cacccacatc
      901 cgcacccaca caggcgagaa gccttttgcc tgtgacattt gtgggaggaa gtttgccagg
      961 agtgatgaac gcaagaggca taccaaaatc catacaggtg gttctggcac gcccgctgca
     1021 gcgagcactc tagaggaccc tagtgcggga gacatgcgtg ccgcgaactt gtggcctagt
     1081 cccttgatga ttaaacgttc caagaagaac agcttagcgt tgagtcttac ggctgaccag
     1141 atggtcagcg ctctactaga cgctgaaccc ccgatcttat acagtgagta cgatcctact
     1201 cgtccctttt ctgaggcctc catgatggga cttttgacaa acttggcaga tcgtgaactt
     1261 gttcacatga tcaactgggc gaaaagggtg cccggcttcg tagacttgac tctgcacgac
     1321 caggtgcatt tgctggagtg tgcgtggcta gagattctta tgatcggact ggtctggcgt
     1381 agcatggaac atcccgttaa gctgcttttc gctccaaatc tacttttaga taggaaccag
     1441 ggaaaatgcg ttgaaggcat ggtggagata tttgacatgc tgttggcgac ctcttcccgt
     1501 tttaggatga tgaatctaca aggtgaagag tttgtatgtc taaaaagtat aatactgttg
     1561 aatagtggag tctacacatt cttatccagc accttaaagt ctctagagga gaaagaccac
     1621 atacaccgtg ttctagacaa aatcaccgat acattaattc accttatggc taaggctggg
     1681 cttacgctac aacaacagca ccaaagatta gcgcaactat tactaattct gtctcacatc
     1741 agacacatgt caaacaaagg tatggaacac ctttattcta tgaaatgcaa aaacgttgta
     1801 ccgttgtacg atctactgct tgagatgcta gacgctcaca gacttcacgc tccaacaagt
     1861 aggggagggg cttccgtaga agagactgac cagtcacatt tggcgacggc tggtagcacc
     1921 tcctcgggat ccgccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc
     1981 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     2041 gacggggatt ccccggggcc gggatttacc ccccacgact ccgcccccta cggcgctctg
     2101 gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac
     2161 ggtgggtaac tcgagtggca gtgcttttaa ctaagaatta ttagtctttt ctgcttattt
     2221 tttcatcata gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta
     2281 aaaattgata cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg
     2341 cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa
     2401 aaatccaaac cagatgtcaa cacagctaca acgggtaccg ggcccttata ttgaattttc
     2461 aaaaattctt actttttttt tggatggacg caaagaagtt taataatcat attacatggc
     2521 attaccacca tatacatatc catatctaat cttacttata tgttgtggaa atgtaaagag
     2581 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
     2641 ctcattgcta tattgaagtg aattcggcgt gggcgtgcgt gggcgggcgt gggcgtgcgt
     2701 gggcgggcgt gggcgtgcgt gggcgggatc cccgtgcgtc ctcgtcttca ccggtcgcgt
     2761 tcctgaaacg cagatgtgcc taatgccgca ctgctccgaa caataaagat tctacaatac
     2821 tagcttttat ggttatgaag aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa
     2881 ttaacgaatc aaattaacaa ccataggatg ataatgcgat tagtttttta gccttatttc
     2941 tggggtaatt aatcagcgaa gcgatgattt ttgatctatt aacagatata taaatggaaa
     3001 agctgcataa ccactttaac taatactttc aacattttca gtttgtatta cttcttattc
     3061 aaatgtcata aaagtatcaa caaaaaattg ttaatatacc tctatacttt aacgtcaagg
     3121 agaaaaaact atactcgaga gatctatgga ctacaaagac catgacggtg attataaaga
     3181 tcatgacatc gattacaagg atgacgatga caagggccct aaaaagaagc gtaaagtcgc
     3241 ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat
     3301 ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc
     3361 ggggccggga tttacccccc acgactccgc cccctacggc gctctggata tggccgactt
     3421 cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg ggggcgtgtc
     3481 caaaggagag gagttaatca aggaaaacat gagaatgaaa gttgtcatgg agggctccgt
     3541 taatggtcac caattcaagt gtacagggga aggtgaaggt aatccttaca tgggtacaca
     3601 aactatgaga attaaagtaa ttgaaggcgg accactacca tttgcatttg acattctggc
     3661 aacgtcattc atgtacggat cacgaacttt catcaagtac cctaaaggta taccagactt
     3721 tttcaagcaa tcttttccag agggttttac atgggaaagg gttacaagat acgaagatgg
     3781 gggtgtcgtc acagttatgc aagatacttc attagaagat ggctgccttg tctatcatgt
     3841 gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa agaccaaagg
     3901 ttgggaacca aatactgaaa tgatgtaccc agctgatgga ggtttgagag gctacacaca
     3961 catggcgctt aaagttgatg gtggaggtca tttgtcttgt agttttgtta ccacttatcg
     4021 ttctaaaaag actgttggca atatcaaaat gccaggaata catgctgtag accacagact
     4081 agaaagactc gaagagagcg ataacgaaat gttcgttgta cagagagagc atgccgtagc
     4141 caaatttgct ggcttaggcg gtggtatgga tgaattgtat aagggttctt ctagacccgg
     4201 ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt tcgaccggcc agatccttga
     4261 ccgccatacc cgtactcata ccggtgaaaa accgtttcag tgtcggatct gtatgcgaaa
     4321 tttctccgtg gcgcacagct tgaagaggca tctacgtacg cacaccggcg agaagccatt
     4381 ccaatgccga atatgcatgc gcaacttcag tgaccccagc aacctgcggc gccacctaaa
     4441 aacccacctg agaggatccg gctccggatc tgtcaaagag tcccttgtgt aactcgagtg
     4501 gcgccaattg gtgcggcaat tgataataac gaaaatgtct tttaatgatc tgggtataat
     4561 gaggaatttt ccgaacgttt ttactttata tatatatata catgtaacat atattctata
     4621 cgctatagag aaaggaaatt tttcaattaa aaaaaaaata gagaaagagt ttcacttctt
     4681 gattatcgct aacactaatg gttgaagtac tgctacttta attttatagc agttacagag
     4741 atgttacgaa ccactagtgc actgcagtac agtttagctt gcctcgtccc cgccgggtca
     4801 cccggccagc gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc
     4861 aggggcatga tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt
     4921 tgcatccata cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctcca
     4981 gacctgcgag cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc
     5041 cctgtagaga aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc
     5101 ttttaaaatc ttgctaggat acagttctca catcacatcc gaacataaac aaaaatgggt
     5161 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     5221 gtgtccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     5281 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5341 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5401 gaatttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     5461 gacctgcctg aaaccgaact gcccgctgtt ctgcaaccgg tcgcggaggc catggatgcg
     5521 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5581 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5641 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5701 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5761 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5821 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5881 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     5941 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     6001 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     6061 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     6121 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     6181 taaagtaact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     6241 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     6301 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     6361 tatgtgaatg ctggtcgcta tactggagtt atcgtgttgc atctgcggct ttaaattgat
     6421 gtatctcatc gcaggcacgg gcagtacagt gccctgagcg tagggaaaaa tgaaaaaaag
     6481 gatgtaactt ttaacataat tccagcacgc agcgattggg tataatgaag attgttaagt
     6541 tcaacaacat gtgaaattct tagctccgca acttgcttga attatgagct ctaagattca
     6601 agaagtaaat aggtcatata atggaatgat agtagtactt gataaaggaa ccatgtgatc
     6661 ttacgttgat atgaaaataa ttctctcaca gaagtcagtt tgtatgacta acatagagag
     6721 taattgcttt tcggataaaa tcgcacctaa caaagattac cttttgtaaa gcctccagaa
     6781 cagctatgaa tgttcgttat tctacgcact ttctttcata catcttgatc cgcctttttt
     6841 gatgtaggtt ttaacgcggc aaaacatatt aatttttctg atttggaaaa ataaagagtt
     6901 agttctgccc cgagcggccg cgtgttacaa ccaattaacc aattctgatt agaaaaactc
     6961 atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg
     7021 aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag
     7081 atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc
     7141 ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga
     7201 gaatggcaaa agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc
     7261 gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag
     7321 gcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg
     7381 caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac
     7441 ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg
     7501 gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat
     7561 ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc
     7621 atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc
     7681 ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga
     7741 cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag
     7801 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     7861 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agtcatgacc
     7921 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     7981 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8041 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8101 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8161 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8221 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8281 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8341 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8401 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     8461 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     8521 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     8581 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     8641 tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg ccgccaattc taattgtatc
     8701 gagatcactt ttcgtgatcc gctaatcagc gacggtcaca ttaggtttgc caagtcaggg
     8761 tatgaaccat acgatcagtt ttcgtgaacc tggtacgtat attgtggcgt ttgtgtatat
     8821 tttcattctt tgacaacaat caataccaac ctcaaatagg aaaagtaata agtttggcgt
     8881 tacaccccaa aagacgccaa acggatcgaa cttactcaat agcaattagc gagacaaaac
     8941 ctacgttaag acctgtaacc gatttatcaa agcactctgc ggttctttct tgggaatatt
     9001 acctggacat tttgtgccct caagaaacga ggctctacga gcctgttgga gcccctcaga
     9061 cattagccgc cacgaatcaa actttttacg cgattcggcc caaatcagtt tctcacagat
     9121 cattcgtaga gtgaaaaagc acatcgatta tttgataccc ctttgggtta attactgttg
     9181 aggtctttgc cgtaagttcc atcgctgtcc ctgaattcgc atctaga 
//
