LOCUS       VB220720-1021gmb        6223 bp    DNA     circular SYN 02-APR-2024
DEFINITION  
ACCESSION   VB220720-1021gmb
VERSION     VB220720-1021gmb
KEYWORDS    .
SOURCE      
  ORGANISM  .
            
FEATURES             Location/Qualifiers
     source          1..6223
     ITR             1..130
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional
                     equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /note="color: #f54c6b; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="6d343a478711450d8e5c28ceb27e5b84"
     Promoter        158..1890
                     /application_notes="Strong promoter."
                     /description="CMV early enhancer fused to modified chicken
                     beta-actin promoter"
                     /full_name="Cytomegalovirus early enhancer fused with
                     chicken beta-actin promoter"
                     /label="CAG"
                     /note="color: #944603; direction: RIGHT"
                     /official_designation="CAG"
                     /uuid="420ea94873db443a902370384d17a441"
     Miscellaneous   1915..1920
                     /application_notes="Facilitates translation initiation of
                     ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             1921..2601
                     /application_notes=""
                     /label="{Kaede}"
                     /note="color: #0ed8aa; direction: RIGHT"
                     /official_designation="{Kaede}"
                     /uuid="178faa99230542baae6b336f5ae9aa77"
     Miscellaneous   2632..3229
                     /application_notes="Enhances virus stability in packaging
                     cells, leading to higher titer of packaged virus; enhances
                     higher expression of transgenes."
                     /description="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /full_name="Woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="WPRE"
                     /uuid="0aedff8308474b369c622433538d7836"
     PolyA_signal    3260..3467
                     /application_notes="Allows transcription termination and
                     polyadenylation of mRNA transcribed by Pol II RNA
                     polymerase."
                     /description="Bovine growth hormone polyadenylation signal"
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3475..3604)
                     /application_notes="Allows rescue of virus from recombinant
                     plasmid and replication of the viral genome; this ITR is
                     identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /note="color: #a949ca; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             4532..5392
                     /application_notes="Allows E. coli to be resistant to
                     ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     Rep_origin      5563..6151
                     /application_notes="Facilitates plasmid replication in E.
                     coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #512bbd; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgctc gacattgatt attgactagt
      181 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      241 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      541 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      601 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
      661 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
      721 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      781 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      841 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      901 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      961 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     1021 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1081 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     1141 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1201 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     1261 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     1321 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     1381 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     1441 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     1501 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     1561 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     1621 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1681 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1741 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1801 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1861 gtgctgtctc atcattttgg caaagaattg caagtttgta caaaaaagca ggctgccacc
     1921 atggtgagtc tgattaaacc agaaatgaag atcaagctgc ttatggaagg caatgtaaac
     1981 gggcaccagt ttgttattga gggagatgga aaaggccatc cttttgaggg aaaacagagt
     2041 atggaccttg tagtcaaaga aggcgcacct ctcccttttg cctacgatat cttgacaaca
     2101 gcattccatt atggtaacag ggtttttgct aaatacccag accatatacc agactacttc
     2161 aagcagtcgt ttcccaaagg gttttcttgg gagcgaagcc tgatgttcga ggacgggggc
     2221 gtttgcatcg ctacaaatga cataacactg aaaggagaca ctttttttaa caaagttcga
     2281 tttgatggcg taaactttcc cccaaatggt cctgttatgc agaagaagac tctgaaatgg
     2341 gaggcatcca ctgagaaaat gtatttgcgt gatggagtgt tgacgggcga tattacaatg
     2401 gctctgctgc ttaaaggaga tgtccattac cgatgtgact tcagaactac ttacaaatct
     2461 aggcaggagg gtgtcaagtt gccaggatat cactttgtcg atcactgcat cagcatattg
     2521 aggcatgaca aagactacaa cgaggttaag ctgtatgagc acgctgttgc ccattctgga
     2581 ttgccggaca acgtcaagta aacccagctt tcttgtacaa agtgggaatt ccgataatca
     2641 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2701 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2761 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2821 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2881 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2941 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3001 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     3061 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3121 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3181 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcctaga
     3241 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3301 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3361 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3421 gacagcaagg gggaggattg ggaagagaat agcaggcatg ctggggaggg ccgcaggaac
     3481 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3541 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3601 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3661 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     3721 gggggtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     3781 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     3841 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     3901 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     3961 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4021 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4081 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4141 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4201 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4261 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4321 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4381 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4741 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5041 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5221 tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga
     5281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6181 cctggccttt tgctggcctt ttgctcacat gtcctgcagg cag
//
