LOCUS       Exported                3699 bp ds-DNA     circular SYN 02-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Macapp
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 2, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          422..1484
                     /organism="pMMB67EH"
                     /mol_type="other DNA"
                     /note="/vntifkey=98"
     source          1485..1778
                     /organism="pMMB67EH"
                     /mol_type="other DNA"
                     /note="/vntifkey=98"
     source          1779..3699
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     source          1779..3699
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     source          1779..3699
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     source          2789..3632
                     /organism="unidentified cloning vector"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:45196"
     promoter        33..63
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     misc_feature    131..133
                     /label=start codon
     misc_feature    134..145
                     /label=Int 1 5' exon
     misc_feature    146..418
                     /label=Int 3
     misc_feature    229..258
                     /label=Theophylline
     misc_feature    419..421
                     /label=Int 1 3' exon
     misc_feature    422..1453
                     /label=cre-E.coli
                     /note="/vntifkey=21"
     terminator      1572..1729
                     /label=rrnB_terminator
                     /note="/vntifkey=43"
     terminator      1692..1778
                     /label=rrnB_T1_terminator
                     /note="/vntifkey=43"
     misc_feature    complement(1779..2741)
                     /label=CmR
                     /label=Cm from pACYC184
     primer_bind     1779..1808
                     /label=Cm-F
     primer_bind     complement(2712..2761)
                     /label=Cm-R
     rep_origin      2789..3632
                     /label=P15A
                     /note="putative"
ORIGIN
        1 taaagcggat aacaatttca cacaggaggc cgtttacact ttatgcttcc ggctcgtata
       61 atgtgtgggg aaaccacaac ggtttccctc tagaaataat tttgtttaac tataagaagg
      121 agatatacat atgtccttgg gttaattgag gcctgagtat aaggtgactt atacttgtaa
      181 tctatctaaa cggggaacct ctctagtaga caatcccgtg ctaaattgat accagcatcg
      241 tcttgatgcc cttggcagca taaatgccta acgactatcc ctttggggag tagggtcaag
      301 tgactcgaaa cgatagacaa cttgctttaa caagttggag atatagtctg ctctgcatgg
      361 tgacatgcag ctggatataa ttccggggta agattaacga ccttatctga acataatgct
      421 aagcaatctg ctgaccgttc accaaaatct gccggcgctg ccggttgatg cgaccagcga
      481 cgaagtgcgt aaaaatctga tggacatgtt ccgtgaccgt caggcgttta gcgagcacac
      541 ctggaagatg ctgctgagcg tgtgccgtag ctgggcggcg tggtgcaagc tgaacaaccg
      601 taaatggttt ccggcggagc cggaagatgt gcgtgattac ctgctgtatc tgcaggcgcg
      661 tggcctggcg gttaaaacca ttcagcaaca cctgggtcaa ctgaacatgc tgcaccgtcg
      721 tagcggcctg ccgcgtccga gcgacagcaa cgcggtgagc ctggttatgc gtcgtatccg
      781 taaggagaac gtggatgcgg gtgaacgtgc gaaacaggcg ctggcgttcg agcgtaccga
      841 ctttgatcaa gttcgtagcc tgatggaaaa cagcgaccgt tgccaagata ttcgtaacct
      901 ggcgtttctg ggcatcgcgt acaacaccct gctgcgtatc gcggagattg cgcgtatccg
      961 tgttaaggac attagccgta ccgatggtgg ccgtatgctg atccacattg gtcgtaccaa
     1021 aaccctggtg agcaccgcgg gtgttgagaa ggcgctgagc ctgggcgtga ccaaactggt
     1081 tgaacgttgg atcagcgtga gcggtgttgc ggacgatccg aacaactacc tgttctgccg
     1141 tgttcgtaag aacggtgttg cggcgccgag cgcgaccagc cagctgagca cccgtgcgct
     1201 ggagggtatt tttgaagcga cccaccgtct gatctacggt gcgaaagacg atagcggcca
     1261 acgttatctg gcgtggagcg gtcacagcgc gcgtgtgggt gcggcgcgtg atatggcgcg
     1321 tgcgggcgtt agcattccgg aaatcatgca ggcgggtggc tggaccaacg tgaacattgt
     1381 tatgaactac attcgtaatc tggatagcga gaccggtgcg atggtgcgcc tgctggagga
     1441 tggtgattag taaggatcct ctagagtcga cctgcaggca tgcaagcttg gctgttttgg
     1501 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     1561 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     1621 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     1681 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1741 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatgt cgaatttgct ttcgaatttc
     1801 tgccattcat ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca
     1861 ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     1921 attaagcatt ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag
     1981 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2041 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2101 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2161 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2221 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2281 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat
     2341 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2401 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2461 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2521 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2581 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     2641 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     2701 atttatttat tctgcgaagt gatcttccgt cacaggtatt ttagctagct cactcggtcg
     2761 ctacgctccg ggcgtgagac tgcggcggta gcggagtgta tactggctta ctatgttggc
     2821 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
     2881 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
     2941 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     3001 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     3061 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     3121 gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg ctctcctgtt
     3181 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     3241 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     3301 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     3361 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     3421 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     3481 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     3541 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     3601 atcatcttat taatcagata aaatatttct agttttctac tgaaccgctc tagatttcag
     3661 tgcaatttat ctcttcaaat gtagcacctg aagtcagcc
//
