LOCUS       pAGD335                 9218 bp    DNA     circular UNA 01-DEC-2023
DEFINITION  Generated by Gibson/Homology Cloning (pAGD319 AGP610-611, pAGD319
            608-609, pAGD319 AGP566-594).
ACCESSION   urn.local...14-g6v37d6
VERSION     urn.local...14-g6v37d6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     source          join(<6277..9218,1..>1243)
                     /organism="Red recombinase plasmid pKD46"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:170495"
     misc_feature    1..1358
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD319 AGP610-611 extraction
                     (concatenated sequence 1)"
     misc_feature    1..>1352
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD318 AGP590-594 extraction
                     (concatenated sequence 1)"
     misc_feature    1..>1352
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD248 AGP590-591 extraction
                     (concatenated sequence 1)"
     CDS             complement(11..889)
                     /codon_start=1
                     /transl_table=11
                     /product="araC protein"
                     /protein_id="AAL02045.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
                     /standard_name="araC protein CDS"
     regulatory      914..1191
                     /note="Geneious type: promoter"
                     /standard_name="araBAD promoter"
     regulatory      918..933
                     /standard_name="O2"
     misc_feature    complement(1040..1068)
                     /note="Pc"
                     /standard_name="Pc"
     regulatory      1055..1071
                     /standard_name="O1L"
     regulatory      1076..1091
                     /standard_name="O1R"
     primer_bind     1123..1142
                     /standard_name="pBAD-For"
     regulatory      1129..1145
                     /standard_name="I1"
     regulatory      1150..1166
                     /standard_name="I2"
     misc_feature    1165..1192
                     /note="ParaB"
                     /standard_name="ParaB"
     misc_feature    1223..1243
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(1223..>1352,<6143..6294)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD201 AGP490-491 (concatenated sequence
                     2)"
     primer_bind     1243..1266
                     /standard_name="AGP690"
     misc_feature    1244..1253
                     /standard_name="extended 'primer'"
     misc_feature    <1244..>1352
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSLS_492 AGP350-351 (concatenated sequence
                     2)"
     misc_RNA        1253..>1323
                     /standard_name="msdRNA"
     misc_feature    complement(1254..1265)
                     /note="Geneious type: primer"
                     /standard_name="primer1"
     misc_feature    complement(1324..>1352)
                     /note="Geneious type: msd"
                     /standard_name="msd"
     misc_RNA        <1324..>1352
                     /product="msr"
                     /function="primer for the Ec86 RT"
                     /standard_name="msr86"
     primer_bind     complement(1325..1352)
                     /standard_name="AGP691"
     primer_bind     complement(1327..1352)
                     /standard_name="AGP610"
     misc_feature    1333..1358
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1333..2452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD319 608-609 (concatenated sequence 2)"
     primer_bind     1359..1383
                     /standard_name="AGP608"
     misc_feature    1359..1384
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <1359..>2420
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD236 AGP592-593 (concatenated sequence
                     2)"
     misc_feature    join(2403..>2420,<1359..1384)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD205 AGP477-478 (concatenated sequence
                     1)"
     misc_feature    1359..>2420
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD205 AGP479-480 (concatenated sequence
                     2)"
     misc_feature    <1359..>1384
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSH0546 AGP358-364 (concatenated sequence
                     2)"
     misc_feature    <1359..>1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD159 AGP287-288 extraction
                     (concatenated sequence 1)"
     misc_feature    <1359..>1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="msd Array Csy4 (concatenated sequence 2)"
     misc_feature    <1359..>1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pCDFDuet- AGP72-AGP73 (concatenated
                     sequence 1)"
     misc_feature    <1359..1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD185 AGP278-363 - pAGD151 AGP79-278 -
                     pCDFDuet- AGP72-AGP73 (concatenated sequence 1)
                     (concatenated sequence 1) (concatenated sequence 1)"
     misc_feature    <1359..1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD151 AGP79-278 - pCDFDuet- AGP72-AGP73
                     (concatenated sequence 1) (concatenated sequence 1)"
     misc_feature    <1373..1377
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(<2402..>2406,<1373..>1377)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD159 AGP287-288 extraction
                     (concatenated sequence 1)"
     misc_feature    join(<2402..>2406,<1373..>1377)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="msd Array Csy4 (concatenated sequence 2)"
     misc_feature    <1373..>2406
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSH0546 AGP358-364 (concatenated sequence
                     2)"
     misc_feature    <1373..>1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pCDFDuet- AGP72-AGP73 (concatenated
                     sequence 1)"
     misc_feature    <1373..1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD185 AGP278-363 - pAGD151 AGP79-278 -
                     pCDFDuet- AGP72-AGP73 (concatenated sequence 1)
                     (concatenated sequence 1) (concatenated sequence 1)"
     misc_feature    <1373..1377
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD151 AGP79-278 - pCDFDuet- AGP72-AGP73
                     (concatenated sequence 1) (concatenated sequence 1)"
     sig_peptide     1378..1383
                     /standard_name="BsaI"
     regulatory      1384..1513
                     /note="Geneious type: promoter"
                     /standard_name="BBa_J72163 GlpT Promoter"
     regulatory      1384
                     /note="Geneious type: promoter"
                     /standard_name="BBa_J72163 GlpT Promoter"
     misc_feature    1384..1549
                     /standard_name="E. coli GPDH Promoter"
     misc_feature    1384..1513
                     /standard_name="pglpT-jtk2821"
     misc_feature    1384
                     /standard_name="E. coli GPDH Promoter"
     misc_feature    1384
                     /standard_name="pglpT-jtk2821"
     misc_feature    1514..1549
                     /note="Geneious type: RBS"
                     /standard_name="sfGFP Ribosome Binding Site"
     misc_feature    1532..1543
                     /standard_name="Elowitz RBS (BBa_B0034)(2)"
     CDS             1550..2266
                     /standard_name="sfGFP"
     regulatory      2267..2395
                     /note="Geneious type: terminator"
                     /standard_name="BBa_B0015 Terminator"
     regulatory      2267..2395
                     /note="Geneious type: terminator"
                     /note="T1/TE bidirectional terminator"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T1/TE"
     misc_feature    join(2267..2346,2355..2395)
                     /standard_name="Ec_rrfB + T7_TE (BBa_B0015)"
     stem_loop       2278..2321
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="stem loop"
     stem_loop       2362..2381
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7 TE"
     regulatory      complement(2362..2389)
                     /note="Geneious type: terminator"
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7Te terminator regulatory"
     misc_feature    2382..2395
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="misc"
     sig_peptide     2396..2401
                     /standard_name="BsaI"
     primer_bind     complement(2396..2420)
                     /standard_name="AGP609"
     misc_RNA        <2402..>2403
                     /standard_name="msdRNA"
     misc_feature    2402..>2406
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD185 AGP278-363 extraction
                     (concatenated sequence 3)"
     misc_feature    2421..>2425
                     /note="Geneious type: primer"
                     /standard_name="downstream"
     primer_bind     2421..2452
                     /standard_name="AGP586"
     misc_feature    2421..2452
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <2421..2425
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2421..6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD319 AGP566-594 (concatenated sequence
                     3)"
     misc_feature    <2421..6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD318 AGP590-594 extraction
                     (concatenated sequence 1)"
     misc_feature    2421..>6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD248 AGP586-587 (concatenated sequence
                     2)"
     misc_feature    <2421..>6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD201 AGP490-491 (concatenated sequence
                     2)"
     misc_feature    join(<2421..>2425,<2463..>3425)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSLS_492 AGP352-353 (concatenated sequence
                     3)"
     misc_feature    <2421..2452
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD248 AGP590-591 extraction
                     (concatenated sequence 1)"
     misc_feature    <2421..2425
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSLS_492 AGP350-351 (concatenated sequence
                     2)"
     misc_feature    complement(2449..2454)
                     /note="Geneious type: RBS"
                     /standard_name="RBS"
     gene            2463..3425
                     /standard_name="ec86RT"
     misc_feature    3451..3456
                     /note="Geneious type: RBS"
                     /standard_name="RBS"
     gene            3464..4276
                     /standard_name="CspRecT"
     source          join(<3464..4277,4278..>6142)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    <3464..>6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pORTMAGE-Ec1 AGP354-355 (concatenated
                     sequence 1)"
     gene            4295..6142
                     /standard_name="mutLE32K"
     primer_bind     complement(6117..6142)
                     /standard_name="AGP594"
     misc_feature    6117..6142
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6117..9218
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD319 AGP610-611 extraction
                     (concatenated sequence 4)"
     misc_feature    6117..>6142
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD236 AGP592-593 (concatenated sequence
                     2)"
     source          <6143..>6276
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     6143..6163
                     /standard_name="AGP611"
     misc_feature    6143..6163
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6143..9218
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD318 AGP590-594 - pAGD248 AGP590-591
                     extraction (concatenated sequence 4) (concatenated
                     sequence 3)"
     misc_feature    6143..9218
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD248 AGP590-591 extraction
                     (concatenated sequence 4)"
     misc_feature    <6143..>6276
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pORTMAGE-Ec1 AGP354-355 (concatenated
                     sequence 1)"
     misc_feature    <6143..6163
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD236 AGP592-593 (concatenated sequence
                     2)"
     misc_feature    <6143..6163
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pAGD227 AGP588-589 (concatenated sequence
                     3)"
     regulatory      6177..6271
                     /note="Geneious type: terminator"
                     /note="transcription terminator from phage lambda"
                     /standard_name="lambda t0 terminator"
     misc_feature    6277..6294
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    6277..1243
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pKD46 AGP488-AGP489 (concatenated sequence
                     1)"
     CDS             complement(6385..7335)
                     /codon_start=1
                     /transl_table=11
                     /product="repA101 protein"
                     /protein_id="AAL02049.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK
                     ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK
                     FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK
                     ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL
                     IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS
                     FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /standard_name="repA101 protein CDS"
     rep_origin      7339..8079
     CDS             complement(8227..9087)
                     /note="bla; ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAL02050.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="beta-lactamase CDS"
ORIGIN      
        1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
       61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
      121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
      181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
      241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
      301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat
      361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
      421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
      481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg
      541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
      601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc
      661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca
      721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
      781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
      841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
      901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
      961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
     1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
     1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaagattcc gtatgcgcac
     1261 ccttagcgag aggtttatca ttaaggtcaa cctctggatg ttgtttcggc atcctgcatt
     1321 gaatctgagt tactgtctgt tttcctgaat tctgttggga attcttctag aggtagagag
     1381 accgaaagtg aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     1441 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     1501 aaacggcagg ttcggatctt agctactaga gaaagaggag aaatactaga tgcgtaaagg
     1561 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     1621 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     1681 gaagttcatc tgtactactg gtaaactgcc ggttccttgg ccgactctgg taacgacgct
     1741 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     1801 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     1861 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     1921 gctgaaaggc attgacttta aagaggacgg caatatcctg ggccataagc tggaatacaa
     1981 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     2041 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     2101 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     2161 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     2221 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgaccag gcatcaaata
     2281 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2341 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtct
     2401 cagaaatact agtactgcag gcggcaaata attttgttta actttaagaa ggagatatac
     2461 atatgaaatc cgctgaatat ttgaacactt ttagattgag aaatctcggc ctacctgtca
     2521 tgaacaattt gcatgacatg tctaaggcga ctcgcatatc tgttgaaaca cttcggttgt
     2581 taatctatac agctgatttt cgctatagga tctacactgt agaaaagaaa ggcccagaga
     2641 agagaatgag aaccatttac caaccttctc gagaacttaa agccttacaa ggatgggttc
     2701 tacgtaacat tttagataaa ctgtcgtcat ctcctttttc tattggattt gaaaagcacc
     2761 aatctatttt gaataatgct accccgcata ttggggcaaa ctttatactg aatattgatt
     2821 tggaggattt tttcccaagt ttaactgcta acaaagtttt tggagtgttc cattctcttg
     2881 gttataatcg actaatatct tcagttttga caaaaatatg ttgttataaa aatctgctac
     2941 cacaaggtgc tccatcatca cctaaattag ctaatctaat atgttctaaa cttgattatc
     3001 gtattcaggg ttatgcaggt agtcggggct tgatatatac gagatatgcc gatgatctca
     3061 ccttatctgc acagtctatg aaaaaggttg ttaaagcacg tgatttttta ttttctataa
     3121 tcccaagtga aggattggtt attaactcaa aaaaaacttg tattagtggg cctcgtagtc
     3181 agaggaaagt tacaggttta gttatttcac aagagaaagt tgggataggt agagaaaaat
     3241 ataaagaaat tagagcaaag atacatcata tattttgcgg taagtcttct gagatagaac
     3301 acgttagggg atggttgtca tttattttaa gtgtggattc aaaaagccat aggagattaa
     3361 taacttatat tagcaaatta gaaaaaaaat atggaaagaa ccctttaaat aaagcgaaga
     3421 cctaagatcg aaataatttt gtttaacttt aaggaggttt ggaatgaacc aaatcgtgaa
     3481 gttcactgac gactctggcc tggcggttca agttactcca gacgatgttc gccgttatat
     3541 ctgtgagaac gctactgaaa aagaggtggg cctctttctg caactctgtc agactcaacg
     3601 tctgaatccg tttgtgaaag acgcttacct ggtgaaatac ggcggtgctc cagcttctat
     3661 gattacttcc tatcaagttt ttaaccgtcg cgcgtgtcgt gatgctaact atgatggtat
     3721 caaatctggt gtggttgttc tgcgtgacgg tgatgttgtg cataaacgtg gtgctgcgtg
     3781 ctacaaaaag gcgggtgagg agctcatcgg tggttgggcg gaagttcgct ttaaggatgg
     3841 ccgcgagact gcgtatgctg aggtggcgct cgacgactat tccaccggca aatctaattg
     3901 ggcgaaaatg ccgggtgtta tgatcgaaaa atgcgcgaag gctgctgctt ggcgcctcgc
     3961 gttcccggac acttttcagg gcatgtacgc tgcggaggaa atggatcaag cgcaacagcc
     4021 agaacaggtg cgcgctcagg cggagcaacc agtggatctc cagccaatcc gcgaactctt
     4081 caagccatat tgcgaacact tcggcatcac tccggctgag ggtatgactg ctgtttgtgg
     4141 tgcggtgggc gctgaaggca tgcactctat gaccgagcag caagctcgcc gtgctcgcgc
     4201 ttggatggag gaagaaatgg ctgcgccagc tgtggaagcg gagtatgagg ttgttgacga
     4261 gggcgaggtg ttttaaggat ccaggagaat acacatgcca attcaggtct taccgccaca
     4321 actggcgaac cagattgccg caggtgaggt ggtcgagcga cctgcgtcgg tagtcaaaga
     4381 actagtgaaa aacagcctcg atgcaggtgc gacgcgtatc gatattgata tcgaacgcgg
     4441 tggggcgaaa cttatccgca ttcgtgataa cggctgcggt atcaaaaaag atgagctggc
     4501 gctggcgctg gctcgtcatg ccaccagtaa aatcgcctct ctggacgatc tcgaagccat
     4561 tatcagcctg ggctttcgcg gtgaggcgct ggcgagtatc agttcggttt cccgcctgac
     4621 gctcacttca cgcaccgcag aacagcagga agcctggcag gcctatgccg aagggcgcga
     4681 tatgaacgtg acggtaaaac cggcggcgca tcctgtgggg acgacgctgg aggtgctgga
     4741 tctgttctac aacaccccgg cgcggcgcaa attcctgcgc accgagaaaa ccgaatttaa
     4801 ccacattgat gagatcatcc gccgcattgc gctggcgcgt ttcgacgtca cgatcaacct
     4861 gtcgcataac ggtaaaattg tgcgtcagta ccgcgcagtg ccggaaggcg ggcaaaaaga
     4921 acggcgctta ggcgcgattt gcggcaccgc ttttcttgaa caagcgctgg cgattgaatg
     4981 gcaacacggc gatctcacgc tacgcggctg ggtggccgat ccaaatcaca ccacgcccgc
     5041 actggcagaa attcagtatt gctacgtgaa cggtcgcatg atgcgcgatc gcctgatcaa
     5101 tcacgcgatc cgccaggcct gcgaagacaa actgggggcc gatcagcaac cggcatttgt
     5161 gttgtatctg gagatcgacc cacatcaggt ggacgtcaac gtgcaccccg ccaaacacga
     5221 agtgcgtttc catcagtcgc gtctggtgca tgattttatc tatcagggcg tgctgagcgt
     5281 gctacaacag caactggaaa cgccgctacc gctggacgat gaaccccaac ctgcaccgcg
     5341 ttccattccg gaaaaccgcg tggcggcggg gcgcaatcac tttgcagaac cggcagctcg
     5401 tgagccggta gctccgcgct acactcctgc gccagcatca ggcagtcgtc cggctgcccc
     5461 ctggccgaat gcgcagccag gctaccagaa acagcaaggt gaagtgtatc gccagctttt
     5521 gcaaacgccc gcgccgatgc aaaaattaaa agcgccggaa ccgcaggaac ctgcacttgc
     5581 ggcgaacagt cagagttttg gtcgggtact gactatcgtc cattccgact gtgcgttgct
     5641 ggagcgcgac ggcaacattt cacttttatc cttgccagtg gcagaacgtt ggctgcgtca
     5701 ggcacaattg acgccgggtg aagcgcccgt ttgcgcccag ccgctgctga ttccgttgcg
     5761 gctaaaagtt tctgccgaag aaaaatcggc attagaaaaa gcgcagtctg ccctggcgga
     5821 attgggtatt gatttccagt cagatgcaca gcatgtgacc atcagggcag tgcctttacc
     5881 cttacgccaa caaaatttac aaatcttgat tcctgaactg ataggctacc tggcgaagca
     5941 gtccgtattc gaacctggca atattgcgca gtggattgca cgaaatctga tgagcgaaca
     6001 tgcgcagtgg tcaatggcac aggccataac cctgctggcg gacgtggaac ggttatgtcc
     6061 gcaacttgtg aaaacgccgc cgggtggtct gttacaatct gttgatttac atccggcgat
     6121 aaaagccctg aaagatgagt gagtcgtgac tgggaaaacc ctggcgacta gtcttggact
     6181 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     6241 ttgccgccgg gcgtttttta ttggtgagaa tccaggtcca tgggtatgga cagttttccc
     6301 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     6361 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     6421 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg
     6481 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     6541 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     6601 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     6661 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     6721 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     6781 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     6841 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     6901 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     6961 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     7021 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     7081 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     7141 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     7201 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     7261 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     7321 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     7381 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     7441 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     7501 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     7561 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     7621 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     7681 aaaccctaaa ggcttaagta gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat
     7741 cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac
     7801 attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc
     7861 cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc
     7921 tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag
     7981 ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag
     8041 actggctaat gcacccagta aggcagcggt atcatcaacg gggtctgacg ctcagtggaa
     8101 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8161 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8221 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8281 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8341 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8401 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8461 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8521 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8581 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8641 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8701 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8761 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8821 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8881 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8941 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9001 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9061 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9121 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9181 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
