LOCUS       pSCL452                 5667 bp    DNA     circular UNA 22-AUG-2022
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...5ez-f26ol3d
VERSION     urn.local...5ez-f26ol3d
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     terminator      15..373
                     /label="tGal7"
     primer_bind     15..50
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCATATTTTTT"
                     /Tm_(binding_region)="59.0"
                     /Tm_(with_extension)="60.2"
                     /%GC_(binding_region)="19.4"
                     /%GC_(with_extension)="26.0"
                     /Hairpin_Tm_(with_extension)="37.9"
                     /Self_Dimer_Tm_(with_extension)="13.6"
                     /Extension="TATCACCTGCACTA"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL507
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL507"
     primer_bind     15..42
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCA"
                     /Tm_(binding_region)="56.1"
                     /Tm_(with_extension)="59.7"
                     /%GC_(binding_region)="25.0"
                     /%GC_(with_extension)="31.0"
                     /Hairpin_Tm_(with_extension)="37.9"
                     /Self_Dimer_Tm_(with_extension)="13.6"
                     /Extension="TATCACCTGCACTA"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL457
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL457"
     primer_bind     15..50
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCATATTTTTT"
                     /Tm_(binding_region)="59.0"
                     /Tm_(with_extension)="74.5"
                     /%GC_(binding_region)="19.4"
                     /%GC_(with_extension)="34.7"
                     /Extension="CTAAGAAACAGGGCAGGTGCTGTATCACCTGCACTA"
                     /modified_by="slopez"
                     /label="oSCL509"
     primer_bind     complement(92..111)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.2"
                     /created_by="primer3"
                     /Sequence="TTGTCACTCCGTTCAAGTCG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pSCL24_alt"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL057
                     (<a href=""urn:local:.:16-cjzjbu1"">pSCL24_alt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a
                     href=""urn:local:.:4i-cwqssks"">pSCL24_alt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL78"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL057
                     (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:a1-ekqc2qq"">pSCL315</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /label="SCL057"
     promoter        complement(387..405)
                     /label="T7_promoter"
     misc_feature    417..431
                     /label="lacZ_a"
     CDS             432
                     /label="lacZα-Broken"
     ligation        482..501
                     /label="Ligation"
     misc_feature    482..501
                     /Color="-1"
                     /label="hom"
     promoter        539..557
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        575..843
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
                     /label="SNR52 promoter"
     extention       844..858
                     /created_by="slopez"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL28"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; extended
                     primer (<a href=""urn:local:.:4f-d4vrki8"">pSCL28</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:8m-eqgasv9"">pSCL370_ins</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:9a-eqgav2m"">pSCL370</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:ze-enrkt54"">pSCL185</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:18f-enrq2x4"">pSCL345</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:15z-enrpddr"">pSCL344</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:12e-enrmj96"">185_ins</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a href=""urn:local:.:5z-d4vrz43"">eco1
                     lp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:1a9-enrqh0n"">pSCL346</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:18t-enrqbru"">pSCL346_ins</a>)"
                     /label="extended primer"
     misc_feature    859..940
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="msr"
     extention       860..871
                     /created_by="slopez"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="485-486"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; primer
                     (<a href=""urn:local:.:449-d5r4g8j"">485-486</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:461-d5r4t0d"">452-471 -
                     485-486</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:5z-d4vrz43"">eco1 lp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:7b-d4vsad2"">eco1 wt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:4f-d4vrki8"">pSCL28</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:2o-ckphag8"">pSCL28</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:1o-cjuf1u1"">pSLS.406</a>)"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL82"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; primer
                     (<a href=""urn:local:.:1jl-d6aabi9"">pSCL82</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:1jl-d6aabi9"">pSCL82</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:dg-df5f1xu"">pSCL126</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:dg-df5f1xu"">pSCL126</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:bv-eu5sepd"">pSCL406</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:bv-eu5sepd"">pSCL406</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:47-etroamr"">pSCL400</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:9c-eq12987"">pSCL367</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:9c-eq12987"">pSCL367</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:63-etroo4a"">pSCL401</a>)"
                     /label="primer"
     misc_RNA        complement(930..>940)
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="msd"
     motif           930..>940
                     /created_by="User"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL38"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Flanking
                     (<a href=""urn:local:.:2y-cppwzgn"">pSCL38</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:l-cxaxuiv"">pSLS.102</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a
                     href=""urn:local:.:d-ckpf78q"">pKDC.009_CLONED</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:i-cppv4as"">pSCL27</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:ud-cylmc5i"">319-320</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:q-cppvdfc"">pSCL27
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:6d-coqizho"">pSCL37</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:5z-d4vrz43"">eco1 lp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a
                     href=""urn:local:.:4q-coqfqly"">pKDC.012_CLONED</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:vc-cylmts8"">pSCL27</a>)"
                     /label="Flanking"
     terminator      941..960
                     /note="transcription terminator for the S. cerevisiae SUP4
                      tRNA gene"
                     /label="SUP4 terminator"
     terminator      966..>995
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /label="CYC1 terminator"
     rep_origin      1070..1525
                     /snapgene_direction="RIGHT"
                     /label="f1 ori"
     CDS             complement(1656..2459)
                     /label="URA3"
     promoter        complement(2460..2680)
                     /label="URA3 promoter"
     misc_feature    join(2945..3358,3359..3448)
                     /label="CEN/ARS"
     promoter        3485..3589
                     /label="AmpR promoter"
     CDS             3590..3658
                     /label="AmpR"
     CDS             3659..4450
                     /label="AmpR"
     rep_origin      4621..5209
                     /snapgene_direction="RIGHT"
                     /label="ori"
     promoter        5265..5625
                     /label="pGal7"
     primer_bind     5549..5570
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /created_by="primer3"
                     /Sequence="AGGTCGGAAATATTTATGGGCA"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pSCL78"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL251
                     (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:i-cppv4as"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:42w-d5qydkl"">pSCL77</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:31h-d5h53rm"">pSCL73</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:479-d5r569u"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:q-cppvdfc"">pSCL27
                     (reversed)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:vc-cylmts8"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:49o-d5r8z74"">pSCL79</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL80"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL251
                     (<a href=""urn:local:.:4dm-d5utwmk"">pSCL80</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:479-d5r569u"">pSCL27</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:3iy-d5l0qh4"">pSCL74</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:q6-dc0im63"">pSCL101</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:42w-d5qydkl"">pSCL77</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:49o-d5r8z74"">pSCL79</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a
                     href=""urn:local:.:2ct-d9qutxn"">pSCL92--re-design?</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:31h-d5h53rm"">pSCL73</a>)"
                     /label="SCL251"
     primer_bind     complement(5593..5625)
                     /created_by="slopez"
                     /Sequence="TTTTGAGGGAATATTCAACTGTTTTTTTTTATC"
                     /Tm_(binding_region)="59.5"
                     /Tm_(with_extension)="77.6"
                     /%GC_(binding_region)="24.2"
                     /%GC_(with_extension)="41.3"
                     /Extension="CAGCACCTGCCCTGTTTCTTAGCTGCCTATACGGCAGTGAAC"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL506
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL506"
     primer_bind     complement(5601..5625)
                     /created_by="slopez"
                     /Sequence="TTTTGAGGGAATATTCAACTGTTTT"
                     /Tm_(binding_region)="55.9"
                     /Tm_(with_extension)="78.0"
                     /%GC_(binding_region)="28.0"
                     /%GC_(with_extension)="44.8"
                     /Extension="CAGCACCTGCCCTGTTTCTTAGCTGCCTATACGGCAGTGAAC"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL456
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL456"
     motif           5626..5653
                     /created_by="slopez"
                     /modified_by="slopez"
                     /X-Transferred_From="pSCL395"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:58l-f26jxi6"">o456-o457</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="paqcI"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:59l-f26kmic"">paqcI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /label="Csy4 site (SCL annotated)"
ORIGIN      
        1 tatcacctgc actaaaagaa agtggaatat tcattcatat catatttttt ctattaactg
       61 cctggtttct tttaaatttt ttattggttg tcgacttgaa cggagtgaca atatatatat
      121 atatatattt aataatgaca tcattatctg taaatctgat tcttaatgct attctagtta
      181 tgtaagagtg gtcctttcca taaaaaaaaa aaaaaagaaa aaagaatttt aggaatacaa
      241 tgcagcttgt aagtaaaatc tggaatattc atatcgccac aacttcttat gcttataaaa
      301 gcactaatgc ctgaatttat gttgaaaata tgtgtcacaa ataaagaaac tgtgacatct
      361 gacacatttc cacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
      421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
      481 agcacatccc cctttcgcca gcaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      541 ttaaccctca ctaaagggaa caaaagctgg agcttctttg aaaagataat gtatgattat
      601 gctttcactc atatttatac agaaacttga tgttttcttt cgagtatata caaggtgatt
      661 acatgtacgt ttgaagtaca actctagatt ttgtagtgcc ctcttgggct agcggtaaag
      721 gtgcgcattt tttcacaccc tacaatgttc tgttcaaaag attttggtca aacgctgtag
      781 aagtgaaagt tggtgcgcat gtttcggcgt tcgaaacttc tccgcagtga aagataaatg
      841 atctgataag attccgtaat gcgcaccctt agcgagaggt ttatcattaa ggtcaacctc
      901 tggatgttgt ttcggcatcc tgcattgaat ctgagttact tttttttgtt ttttatgtct
      961 tcgagtcatg taattagtta tgtcacgctt acgttctggc gtaatagcga agaggcccgc
     1021 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt
     1081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     1141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     1321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     1441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1501 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg
     1561 gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct aatttgtgag
     1621 tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc gcatcttctc
     1681 aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc atcccttccc
     1741 tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac cacatcatcc
     1801 acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac accgggtgtc
     1861 ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc aataaagccg
     1921 ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata ttctccagta
     1981 gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg ttcctttgtt
     2041 acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac accgtgtgca
     2101 ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg caatttgact
     2161 gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta cttggcggat
     2221 aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat atccacatgt
     2281 gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa ttccttggtg
     2341 gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat attaaatagc
     2401 ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc tttcgacatg
     2461 atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa
     2521 tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct gtgctccttc
     2581 cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaaag aaaccgaaat
     2641 caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt atggtgcact
     2701 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     2761 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2821 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     2881 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2941 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
     3001 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
     3061 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
     3121 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
     3181 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
     3241 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
     3301 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
     3361 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
     3421 aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa
     3481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     3601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     3661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     3721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     3841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     4321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     4501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4741 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5221 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcaagtag agggggtaat
     5281 ttttcccctt tattttgttc atacattctt aaattgcttt gcctctcctt ttggaaagct
     5341 atacttcgga gcactgttga gcgaaggctc attagatata ttttctgtca ttttccttaa
     5401 cccaaaaata agggaaaggg tccaaaaagc gctcggacaa ctgttgaccg tgatccgaag
     5461 gactggctat acagtgttca caaaatagcc aagctgaaaa taatgtgtag ctatgttcag
     5521 ttagtttggc tagcaaagat ataaaagcag gtcggaaata tttatgggca ttattatgca
     5581 gagcatcaac atgataaaaa aaaacagttg aatattccct caaaagttca ctgccgtata
     5641 ggcagctaag aaacagggca ggtgctg
//
