LOCUS       pSCL475                 6574 bp    DNA     circular UNA 14-OCT-2023
DEFINITION  Generated by Golden Gate/Type IIS Cloning (pSCL452, ade2_msd_csy4,
            can1_msd_csy4, faa1_msd_csy4).
ACCESSION   urn.local...py-f7hfcaa
VERSION     urn.local...py-f7hfcaa
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    join(6161..6574,1..90)
                     /label="CEN/ARS"
     promoter        127..231
                     /label="AmpR promoter"
     CDS             232..300
                     /label="AmpR"
     CDS             301..1092
                     /label="AmpR"
     rep_origin      1263..1851
                     /snapgene_direction="RIGHT"
                     /label="ori"
     promoter        1907..2267
                     /label="pGal7"
     primer_bind     2191..2212
                     /%GC="40.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /created_by="primer3"
                     /Sequence="AGGTCGGAAATATTTATGGGCA"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pSCL78"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL251
                     (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:i-cppv4as"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:42w-d5qydkl"">pSCL77</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:31h-d5h53rm"">pSCL73</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:479-d5r569u"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:q-cppvdfc"">pSCL27
                     (reversed)</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:vc-cylmts8"">pSCL27</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:49o-d5r8z74"">pSCL79</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL80"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL251
                     (<a href=""urn:local:.:4dm-d5utwmk"">pSCL80</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:479-d5r569u"">pSCL27</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:3iy-d5l0qh4"">pSCL74</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:q6-dc0im63"">pSCL101</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:42w-d5qydkl"">pSCL77</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:49o-d5r8z74"">pSCL79</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a
                     href=""urn:local:.:2ct-d9qutxn"">pSCL92--re-design?</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL251 (<a href=""urn:local:.:31h-d5h53rm"">pSCL73</a>)"
                     /label="SCL251"
     primer_bind     complement(2235..2267)
                     /created_by="slopez"
                     /Sequence="TTTTGAGGGAATATTCAACTGTTTTTTTTTATC"
                     /Tm_(binding_region)="59.5"
                     /Tm_(with_extension)="77.6"
                     /%GC_(binding_region)="24.2"
                     /%GC_(with_extension)="41.3"
                     /Extension="CAGCACCTGCCCTGTTTCTTAGCTGCCTATACGGCAGTGAAC"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL506
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL506"
     primer_bind     complement(2243..2267)
                     /created_by="slopez"
                     /Sequence="TTTTGAGGGAATATTCAACTGTTTT"
                     /Tm_(binding_region)="55.9"
                     /Tm_(with_extension)="78.0"
                     /%GC_(binding_region)="28.0"
                     /%GC_(with_extension)="44.8"
                     /Extension="CAGCACCTGCCCTGTTTCTTAGCTGCCTATACGGCAGTGAAC"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL456
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL456"
     motif           2268..2295
                     /created_by="slopez"
                     /modified_by="slopez"
                     /X-Transferred_From="pSCL395"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:58l-f26jxi6"">o456-o457</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="paqcI"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:59l-f26kmic"">paqcI</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:no-esba25d"">pSCL396</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /label="Csy4 site (SCL annotated)"
     misc_feature    2292..2608
                     /Source="referenceToDocument: urn:local:.:5fy-f26p33b"
                     /label="ade2_msd_csy4 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <2292..2295
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2292..2295
                     /label="Ligation"
     misc_RNA        2296..2439
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="msd"
     misc_feature    <2296..2306
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="msr"
     CDS             2319..2418
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="Ade2 donor"
     CDS             2439
                     /created_by="slopez"
                     /label="RT start"
     misc_feature    complement(2441..2452)
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="inverted repeat"
     misc_feature    complement(2453..2467)
                     /Color="-1"
                     /label="long primer"
     misc_difference 2468..2485
                     /created_by="Seth"
                     /label="gRNA (18 bases)"
     misc_feature    2486..2576
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /label="modified SpCas9 scaffold"
     motif           2577..2604
                     /created_by="slopez"
                     /modified_by="slopez"
                     /X-Transferred_From="pRC04 _array"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL395"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /label="Csy4 site (SCL annotated)"
     misc_feature    2605..>2608
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <2605..2608
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2605..2608
                     /label="Ligation"
     misc_feature    2605..2921
                     /Source="referenceToDocument: urn:local:.:5ns-f26rtnb"
                     /label="can1_msd_csy4 (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_RNA        2609..2752
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="msd"
     misc_feature    <2609..2619
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="msr"
     donor           2632..2731
                     /created_by="slopez"
                     /label="donor"
     misc_feature    2632..2731
                     /Original_Bases="TGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAAC
                     GAAATTGCCTGAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTT
                     C"
                     /label="TGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCC
                     TGAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTC"
                     /note="Geneious type: Editing History Replacement"
     grna            2667..2684
                     /created_by="slopez"
                     /label="can1 gRNA"
     CDS             2752
                     /created_by="slopez"
                     /label="RT start"
     misc_feature    complement(2754..2765)
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="inverted repeat"
     misc_feature    complement(2766..2780)
                     /Color="-1"
                     /label="long primer"
     grna            2781..2798
                     /created_by="slopez"
                     /label="can1 gRNA"
     misc_feature    2781..2798
                     /Original_Bases="ATTAACGAAATTGCCCCA"
                     /label="ATTAACGAAATTGCCCCA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    2799..2889
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /label="modified SpCas9 scaffold"
     motif           2890..2917
                     /created_by="slopez"
                     /modified_by="slopez"
                     /X-Transferred_From="pRC04 _array"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL395"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /label="Csy4 site (SCL annotated)"
     misc_feature    2918..>2921
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    <2918..2921
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        2918..2921
                     /label="Ligation"
     misc_feature    2918..3234
                     /Source="referenceToDocument: urn:local:.:5j5-f26qkw1"
                     /label="faa1_msd_csy4 (concatenated sequence 4)"
                     /note="Geneious type: Concatenated sequence"
     misc_RNA        2922..3065
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="msd"
     misc_feature    <2922..2932
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="msr"
     donor           2945..3044
                     /created_by="slopez"
                     /label="donor"
     misc_feature    2945..3044
                     /Original_Bases="TGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAAC
                     GAAATTGCCTGAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTT
                     C"
                     /label="TGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCC
                     TGAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTC"
                     /note="Geneious type: Editing History Replacement"
     grna            2980..2997
                     /created_by="slopez"
                     /label="faa1 gRNA"
     mutation        complement(2998..3000)
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="change"
     CDS             3065
                     /created_by="slopez"
                     /label="RT start"
     misc_feature    complement(3067..3078)
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="inverted repeat"
     misc_feature    complement(3079..3093)
                     /Color="-1"
                     /label="long primer"
     misc_feature    3094..3111
                     /Original_Bases="ATTAACGAAATTGCCCCA"
                     /label="ATTAACGAAATTGCCCCA"
                     /note="Geneious type: Editing History Replacement"
     grna            3094..3111
                     /created_by="slopez"
                     /label="faa1 gRNA"
     misc_feature    3112..3202
                     /ApEinfo_fwdcolor="#7ea7d8"
                     /ApEinfo_revcolor="#7ea7d8"
                     /label="modified SpCas9 scaffold"
     motif           3203..3230
                     /created_by="slopez"
                     /modified_by="slopez"
                     /X-Transferred_From="pRC04 _array"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Csy4 site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL395"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1h8-eri0d7x"">pSCL395</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:bg-erc3gj7"">pRC04 _array</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:1gm-eri0c7r"">o324-o325</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Csy4
                     site (SCL annotated) (<a
                     href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /label="Csy4 site (SCL annotated)"
     misc_feature    3231..>3234
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        3231..3234
                     /label="Ligation"
     misc_feature    3231..2295
                     /Source="referenceToDocument: urn:local:.:5ez-f26ol3d"
                     /label="pSCL452 (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     terminator      3231..3589
                     /label="tGal7"
     primer_bind     3231..3266
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCATATTTTTT"
                     /Tm_(binding_region)="59.0"
                     /Tm_(with_extension)="60.2"
                     /%GC_(binding_region)="19.4"
                     /%GC_(with_extension)="26.0"
                     /Hairpin_Tm_(with_extension)="37.9"
                     /Self_Dimer_Tm_(with_extension)="13.6"
                     /Extension="TATCACCTGCACTA"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL507
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL507"
     primer_bind     3231..3258
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCA"
                     /Tm_(binding_region)="56.1"
                     /Tm_(with_extension)="59.7"
                     /%GC_(binding_region)="25.0"
                     /%GC_(with_extension)="31.0"
                     /Hairpin_Tm_(with_extension)="37.9"
                     /Self_Dimer_Tm_(with_extension)="13.6"
                     /Extension="TATCACCTGCACTA"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL451"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; oSCL457
                     (<a href=""urn:local:.:4xq-f269tnw"">pSCL451</a>)"
                     /label="oSCL457"
     primer_bind     3231..3266
                     /created_by="slopez"
                     /Sequence="AAAGAAAGTGGAATATTCATTCATATCATATTTTTT"
                     /Tm_(binding_region)="59.0"
                     /Tm_(with_extension)="74.5"
                     /%GC_(binding_region)="19.4"
                     /%GC_(with_extension)="34.7"
                     /Extension="CTAAGAAACAGGGCAGGTGCTGTATCACCTGCACTA"
                     /modified_by="slopez"
                     /label="oSCL509"
     primer_bind     complement(3308..3327)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.2"
                     /created_by="primer3"
                     /Sequence="TTGTCACTCCGTTCAAGTCG"
                     /Mismatches=0
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pSCL24_alt"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL057
                     (<a href=""urn:local:.:16-cjzjbu1"">pSCL24_alt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a
                     href=""urn:local:.:4i-cwqssks"">pSCL24_alt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL78"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SCL057
                     (<a href=""urn:local:.:3z6-d5qxtf9"">pSCL78</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:kc-erhsijt"">pSCL391</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:a1-ekqc2qq"">pSCL315</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     SCL057 (<a href=""urn:local:.:10p-d55cf47"">pSCL70</a>)"
                     /label="SCL057"
     promoter        complement(3603..3621)
                     /label="T7_promoter"
     misc_feature    3633..3647
                     /label="lacZ_a"
     CDS             3648
                     /label="lacZα-Broken"
     ligation        3698..3717
                     /label="Ligation"
     misc_feature    3698..3717
                     /Color="-1"
                     /label="hom"
     promoter        3755..3773
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     promoter        3791..4059
                     /note="promoter for the S. cerevisiae small nucleolar RNA
                     gene SNR52"
                     /label="SNR52 promoter"
     extention       4060..4074
                     /created_by="slopez"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL28"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; extended
                     primer (<a href=""urn:local:.:4f-d4vrki8"">pSCL28</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:8m-eqgasv9"">pSCL370_ins</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:9a-eqgav2m"">pSCL370</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:ze-enrkt54"">pSCL185</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:18f-enrq2x4"">pSCL345</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:15z-enrpddr"">pSCL344</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:12e-enrmj96"">185_ins</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a href=""urn:local:.:5z-d4vrz43"">eco1
                     lp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:1a9-enrqh0n"">pSCL346</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     extended primer (<a
                     href=""urn:local:.:18t-enrqbru"">pSCL346_ins</a>)"
                     /label="extended primer"
     misc_feature    4075..4156
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /Transferred_From="CRISPEY_retron-guide"
                     /Transferred_Similarity="100.00%"
                     /label="msr"
     extention       4076..4087
                     /created_by="slopez"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="485-486"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; primer
                     (<a href=""urn:local:.:449-d5r4g8j"">485-486</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:461-d5r4t0d"">452-471 -
                     485-486</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:5z-d4vrz43"">eco1 lp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:7b-d4vsad2"">eco1 wt</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:4f-d4vrki8"">pSCL28</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:2o-ckphag8"">pSCL28</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:1o-cjuf1u1"">pSLS.406</a>)"
                     /modified_by="slopez"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL82"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; primer
                     (<a href=""urn:local:.:1jl-d6aabi9"">pSCL82</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:1jl-d6aabi9"">pSCL82</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:dg-df5f1xu"">pSCL126</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:dg-df5f1xu"">pSCL126</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:bv-eu5sepd"">pSCL406</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:bv-eu5sepd"">pSCL406</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:47-etroamr"">pSCL400</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:9c-eq12987"">pSCL367</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:9c-eq12987"">pSCL367</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     primer (<a href=""urn:local:.:63-etroo4a"">pSCL401</a>)"
                     /label="primer"
     misc_RNA        complement(4146..>4156)
                     /created_by="slopez"
                     /modified_by="slopez"
                     /label="msd"
     motif           4146..>4156
                     /created_by="User"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSCL38"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Flanking
                     (<a href=""urn:local:.:2y-cppwzgn"">pSCL38</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:l-cxaxuiv"">pSLS.102</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a
                     href=""urn:local:.:d-ckpf78q"">pKDC.009_CLONED</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:i-cppv4as"">pSCL27</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:ud-cylmc5i"">319-320</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:q-cppvdfc"">pSCL27
                     (reversed)</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:6d-coqizho"">pSCL37</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:5z-d4vrz43"">eco1 lp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a
                     href=""urn:local:.:4q-coqfqly"">pKDC.012_CLONED</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Flanking (<a href=""urn:local:.:vc-cylmts8"">pSCL27</a>)"
                     /label="Flanking"
     terminator      4157..4176
                     /note="transcription terminator for the S. cerevisiae SUP4
                      tRNA gene"
                     /label="SUP4 terminator"
     terminator      4182..>4211
                     /gene="S. cerevisiae CYC1"
                     /note="transcription terminator for CYC1"
                     /label="CYC1 terminator"
     rep_origin      4286..4741
                     /snapgene_direction="RIGHT"
                     /label="f1 ori"
     CDS             complement(4872..5675)
                     /label="URA3"
     promoter        complement(5676..5896)
                     /label="URA3 promoter"
ORIGIN      
        1 ttttctttaa tttctttttt tactttctat ttttaattta tatatttata ttaaaaaatt
       61 taaattataa ttatttttat agcacgtgat gaaaaggacc caggtggcac ttttcgggga
      121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     1441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcaagt agagggggta
     1921 atttttcccc tttattttgt tcatacattc ttaaattgct ttgcctctcc ttttggaaag
     1981 ctatacttcg gagcactgtt gagcgaaggc tcattagata tattttctgt cattttcctt
     2041 aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac aactgttgac cgtgatccga
     2101 aggactggct atacagtgtt cacaaaatag ccaagctgaa aataatgtgt agctatgttc
     2161 agttagtttg gctagcaaag atataaaagc aggtcggaaa tatttatggg cattattatg
     2221 cagagcatca acatgataaa aaaaaacagt tgaatattcc ctcaaaagtt cactgccgta
     2281 taggcagcta agaaatctga gttactgtct gttttccttg gaaatgttct atttagaaac
     2341 aggggaattg cttattaacg aaattgcctg aaggcctcac aactctggac attataccat
     2401 tgatgcttgc gtcacttcag gaaacccgtt tcttctgacg taagggtgcg catacggaat
     2461 cttatcaatt aacgaaattg ccccagtttc agagctatgc tggaaacagc atagcaagtt
     2521 gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttgttc
     2581 actgccgtat aggcagctaa gaaacctatc tgagttactg tctgttttcc tgcagttttc
     2641 gttactgctg catttggcgc tttggcttac atggagacat ctacttgagg tgacaaagtt
     2701 ttcgaatggc tattaaatat cactggtgtt gaggaaaccc gtttcttctg acgtaagggt
     2761 gcgcatacgg aatcttatca ttacatggag acatctacgt ttcagagcta tgctggaaac
     2821 agcatagcaa gttgaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     2881 gtgctttttg ttcactgccg tataggcagc taagaaacct atctgagtta ctgtctgttt
     2941 tcctcaggat tctttaccta gcttcaagat gtcgtcaaag ctaaaggtct tgatatctca
     3001 tctaacttct ttaattctgt tgatggcatc atgagcagat tgataggaaa cccgtttctt
     3061 ctgacgtaag ggtgcgcata cggaatctta tcagctaaag gtcttgatat cgtttcagag
     3121 ctatgctgga aacagcatag caagttgaaa taaggctagt ccgttatcaa cttgaaaaag
     3181 tggcaccgag tcggtgcttt ttgttcactg ccgtataggc agctaagaaa aaagaaagtg
     3241 gaatattcat tcatatcata ttttttctat taactgcctg gtttctttta aattttttat
     3301 tggttgtcga cttgaacgga gtgacaatat atatatatat atatttaata atgacatcat
     3361 tatctgtaaa tctgattctt aatgctattc tagttatgta agagtggtcc tttccataaa
     3421 aaaaaaaaaa aagaaaaaag aattttagga atacaatgca gcttgtaagt aaaatctgga
     3481 atattcatat cgccacaact tcttatgctt ataaaagcac taatgcctga atttatgttg
     3541 aaaatatgtg tcacaaataa agaaactgtg acatctgaca catttccacg tacccaattc
     3601 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     3661 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagcag
     3721 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     3781 agctggagct tctttgaaaa gataatgtat gattatgctt tcactcatat ttatacagaa
     3841 acttgatgtt ttctttcgag tatatacaag gtgattacat gtacgtttga agtacaactc
     3901 tagattttgt agtgccctct tgggctagcg gtaaaggtgc gcattttttc acaccctaca
     3961 atgttctgtt caaaagattt tggtcaaacg ctgtagaagt gaaagttggt gcgcatgttt
     4021 cggcgttcga aacttctccg cagtgaaaga taaatgatct gataagattc cgtaatgcgc
     4081 acccttagcg agaggtttat cattaaggtc aacctctgga tgttgtttcg gcatcctgca
     4141 ttgaatctga gttacttttt tttgtttttt atgtcttcga gtcatgtaat tagttatgtc
     4201 acgcttacgt tctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4261 cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4321 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4381 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4441 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4501 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4561 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4621 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4681 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     4741 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     4801 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     4861 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     4921 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     4981 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     5041 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     5101 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     5161 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     5221 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     5281 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     5341 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     5401 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     5461 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     5521 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     5581 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     5641 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcaggtt
     5701 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     5761 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     5821 attaccgaat caaaaaaatt tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     5881 gatgaattga attgaaaagc tgtggtatgg tgcactctca gtacaatctg ctctgatgcc
     5941 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6001 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6061 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     6121 ttataggtta atgtcatgat aataatggtt tcttaggacg gatcgcttgc ctgtaactta
     6181 cacgcgcctc gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat
     6241 ttttatgttt tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat
     6301 gaagaaaaaa aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata
     6361 tatttattag acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa
     6421 tgtaaaatca caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc
     6481 attaatacct gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta
     6541 gagtctttta catcttcgga aaacaaaaac tatt
//
