LOCUS       pSCL758                 7025 bp    DNA     circular UNA 01-AUG-2023
DEFINITION  273_Eco3RT_3site_hCtRNA_EMX1_FANCF_HEK3.
ACCESSION   urn.local...q0-gkwlt1v
VERSION     urn.local...q0-gkwlt1v
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     regulatory      1..364
                     /note="Geneious type: promoter"
                     /gene="CAG Enhancer"
                     /standard_name="CAG enhancer"
     regulatory      365..646
                     /note="Geneious type: promoter"
                     /gene="CAG Promoter"
                     /standard_name="CAG promoter"
     regulatory      647..1525
                     /note="Geneious type: promoter"
                     /gene="CAG Intron"
                     /standard_name="CAG intron"
     misc_feature    1615..>1732
                     /gene="Multiple Cloning Site"
                     /standard_name="MCS"
     misc_feature    1733..3036
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="o944-o945 (concatenated sequence 2)"
     misc_feature    1733..1736
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <1733..1741
                     /standard_name="BbsI_kozak_start"
     misc_feature    1733..>1736
                     /standard_name="BbsI site"
     CDS             <1742..>2686
                     /codon_start=1
                     /standard_name="human codon opt Eco3 RT"
     primer_bind     1742..1765
                     /standard_name="oSCL944"
     misc_feature    complement(2687..2740)
                     /standard_name="hom"
     misc_feature    2720..2740
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2738..3009
                     /note="TY polyA"
     primer_bind     complement(3011..3032)
                     /standard_name="oSCL945"
     misc_feature    3032
                     /note="Geneious type: PCR_primer"
                     /note="PB09 seq rvs"
     misc_feature    3033..3036
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(3033..>3036)
                     /standard_name="BbsI_stuffer"
     misc_feature    <3033..3036
                     /standard_name="BbsI_stuffer"
     misc_feature    3033..3358
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="o946-o947 (concatenated sequence 3)"
     regulatory      3037..3257
                     /note="Geneious type: promoter"
                     /standard_name="H1 Promoter"
     primer_bind     3037..3058
                     /standard_name="oSCL946"
     misc_feature    complement(3222..3257)
                     /note="Geneious type: primer"
                     /standard_name="rev"
     misc_feature    3257^3258
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="CCGAAACGAGCTAGCTCGTCA"
     misc_feature    3258..3272
                     /standard_name="crispey-ext"
     misc_feature    <3273..3347
                     /standard_name="msr"
     misc_feature    3273..3285
                     /standard_name="a2"
     primer_bind     complement(3320..3347)
                     /standard_name="oSCL947"
     misc_feature    complement(3343..>3347)
                     /standard_name="msd"
     misc_feature    complement(3348..>3358)
                     /standard_name="BbsI_stuffer"
     misc_feature    3355..3358
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3355..3730
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3RT_hCtRNA_HEK3 (concatenated sequence
                     4)"
     misc_feature    3355..3357
                     /standard_name="3bp 5' leader"
     misc_feature    3358..3429
                     /standard_name="tRNA-Cys-GCA human"
     misc_feature    <3430..3434
                     /standard_name="msr"
     misc_feature    complement(3430..3601)
                     /standard_name="msd"
     misc_feature    3439..3455
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    3456
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     misc_feature    3457..3576
                     /note="Geneious type: donor"
     misc_feature    3457..3576
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGC
                     AGAAGAAAAAGTTCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAA
                     TGGGGAGGACATCGATGTCA"
     misc_feature    3489..3508
                     /note="Geneious type: gRNA"
     misc_feature    3508
                     /note="Geneious type: change"
                     /standard_name="A -> C"
     misc_feature    3510..3511
                     /note="Geneious type: change"
                     /standard_name="GG -> AA"
     misc_feature    3577
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     misc_feature    complement(3578..3594)
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    complement(3603..3615)
                     /standard_name="a1"
     misc_feature    complement(3616..3630)
                     /standard_name="long primer"
     misc_feature    3631..3650
                     /note="Geneious type: gRNA"
     misc_feature    3632..3649
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGTCCGAGCAGAAGAAGA"
     misc_feature    3650
                     /note="Geneious type: change"
                     /standard_name="A -> C"
     misc_RNA        3651..3726
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="gRNA scaffold"
     misc_feature    3727..3730
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3727..3729
                     /standard_name="3bp 5' leader"
     misc_feature    3727..4102
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3RT_hCtRNA_FANCF (concatenated sequence
                     5)"
     misc_feature    3730
                     /standard_name="tRNA-Cys-GCA human"
     misc_feature    3730..3801
                     /standard_name="tRNA-Cys-GCA human"
     misc_feature    <3802..3806
                     /standard_name="msr"
     misc_feature    complement(3802..3973)
                     /standard_name="msd"
     misc_feature    3811..3827
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    3828
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     misc_feature    3829..3948
                     /note="Geneious type: donor"
     misc_feature    3829..3948
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="ACAAACGGCAGAAGCTGGAGGAGGAAGGGCCTGAGTCCGAGC
                     AGAAGAAAAAGTTCTCCCATCACATCAACCGGTGGCGCATTGCCACGAAGCAGGCCAA
                     TGGGGAGGACATCGATGTCA"
     CDS             <3829..>3948
                     /gene="FANCF"
                     /gene_synonym="FAF"
                     /note="FA complementation group F"
                     /db_xref="GeneID:2188"
                     /db_xref="HGNC:HGNC:3587"
                     /db_xref="MIM:613897"
                     /standard_name="FANCF gene"
     misc_feature    3861..3880
                     /note="Geneious type: gRNA"
     misc_feature    3879
                     /note="Geneious type: mut"
                     /standard_name="C -> T"
     misc_feature    3881..3883
                     /note="Geneious type: pam"
                     /standard_name="PAM"
     misc_feature    3882
                     /note="Geneious type: mut"
                     /standard_name="G -> A"
     misc_feature    3949
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     misc_feature    complement(3950..3966)
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    complement(3975..3987)
                     /standard_name="a1"
     misc_feature    complement(3988..4002)
                     /standard_name="long primer"
     misc_feature    4003..4022
                     /note="Geneious type: gRNA"
     exon            <4003..>4022
                     /gene="FANCF"
                     /gene_synonym="FAF"
                     /inference="alignment:Splign:1.39.8"
                     /number=1
     gene            <4003..>4022
                     /gene="FANCF"
                     /gene_synonym="FAF"
                     /note="FA complementation group F"
                     /db_xref="GeneID:2188"
                     /db_xref="HGNC:HGNC:3587"
                     /db_xref="MIM:613897"
     misc_feature    4004..4022
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="AGTCCGAGCAGAAGAAGAA"
     misc_feature    4021
                     /note="Geneious type: mut"
                     /standard_name="C -> T"
     misc_RNA        4023..4098
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="gRNA scaffold"
     misc_feature    4099..4102
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4099..4101
                     /standard_name="3bp 5' leader"
     misc_feature    4099..4398
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Eco3RT_hCtRNA_EMX1 (concatenated sequence
                     6)"
     misc_feature    4102
                     /standard_name="tRNA-Cys-GCA human"
     misc_feature    4102..4173
                     /standard_name="tRNA-Cys-GCA human"
     misc_feature    <4174..4178
                     /standard_name="msr"
     misc_feature    complement(4174..4345)
                     /standard_name="msd"
     misc_feature    4183..4199
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    4200
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     CDS             <4201..>4320
                     /codon_start=3
                     /gene="EMX1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Gnomon."
                     /product="homeobox protein EMX1 isoform X2"
                     /protein_id="XP_011530999.1"
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
     misc_feature    4201..4320
                     /note="Geneious type: donor"
     misc_feature    4233..4252
                     /note="Geneious type: gRNA"
     misc_feature    4250
                     /note="Geneious type: mut"
                     /standard_name="G -> A"
     misc_feature    4253..4255
                     /note="Geneious type: pam"
                     /standard_name="PAM"
     misc_feature    4254..4255
                     /note="Geneious type: mut"
                     /standard_name="GG -> TT"
     misc_feature    4321
                     /note="Geneious type: feature"
                     /standard_name="stem frag"
     misc_feature    complement(4322..4338)
                     /note="Geneious type: feature"
                     /standard_name="stem"
     misc_feature    complement(4347..4359)
                     /standard_name="a1"
     misc_feature    complement(4360..4374)
                     /standard_name="long primer"
     gene            <4375..>4394
                     /gene="EMX1"
                     /note="empty spiracles homeobox 1; Derived by automated
                     computational analysis using gene prediction method:
                     BestRefSeq,Gnomon."
                     /db_xref="GeneID:2016"
                     /db_xref="HGNC:HGNC:3340"
                     /db_xref="MIM:600034"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    4375..4394
                     /note="Geneious type: gRNA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
     misc_feature    4395..4398
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    complement(4395..>4398)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bbsi"
     misc_feature    4395..1736
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pSCL273 (digested by BbsI and BbsI)
                     (concatenated sequence 1)"
     primer_bind     4395..4419
                     /standard_name="oSCL70"
     misc_feature    4395..4485
                     /standard_name="modified SpCas9 scaffold"
     primer_bind     complement(4466..4485)
                     /standard_name="oSCL71"
     regulatory      complement(4515..4636)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pLac"
     primer_bind     complement(4523..4546)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="M13-R (-46)"
     misc_feature    4713..4732
                     /note="Geneious type: oligo"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40Promoter(20mer)"
     rep_origin      4729..4864
     regulatory      4884..5005
                     /note="Geneious type: terminator"
                     /note="simian vacuolating virus 40 (SV40) polyA signal"
                     /regulatory_class="terminator"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SV40 polyA"
     gene            complement(6016..6876)
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     regulatory      complement(6877..6981)
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2021.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     regulatory      complement(6918..6946)
                     /note="Geneious type: Promoter"
                     /standard_name="amp prom"
ORIGIN      
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
      421 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
      481 gggggggggg gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
      541 aaaggtgcgg cggcagccaa tcaaagcggc gcgctccgaa agtttccttt tatggcgagg
      601 cggcggcggc ggcggcccta taaaaagcga accgcgcggc gggcgggagt cgctgcgcgc
      661 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
      721 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattac
      781 cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct tgaggggctc
      841 cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg tgtgtgcgtg
      901 gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
      961 gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     1021 tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1081 gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc tccccgagtt
     1141 gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc ggggctcgcc
     1201 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     1261 ggggagggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg
     1321 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     1381 tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc
     1441 ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg
     1501 ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc
     1561 cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga
     1621 gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     1681 gttattgtgc tgtctcatca ttttggcaaa gaattgagct cgatatcggt acgccaccat
     1741 gcgcatttac tctctgatcg acagccaaac cttaatgacc aaagggttcg catccgaggt
     1801 catgaggagc ccagaacccc ctaagaagtg ggacattgcg aagaagaagg gcggaatgcg
     1861 tacgatatac catccctctt ctaaggtgaa gctgatacag tactggctga tgaacaacgt
     1921 gttctccaaa ttgccgatgc acaacgccgc gtacgctttc gtgaagaata gatctatcaa
     1981 gtctaacgca ctgctgcacg cagagagtaa gaacaaatac tacgttaaga ttgacctgaa
     2041 ggacttcttt ccaagcatca agttcacaga cttcgaatat gcctttaccc ggtaccgtga
     2101 cagaatagag ttcacgaccg agtacgacaa agaactgctt cagctgatta agaccatttg
     2161 tttcatttct gactctacac tgccaatagg cttccccact tcccctctta tagccaattt
     2221 cgtcgccagg gagctggacg agaagctcac tcagaagctg aacgctatag acaagctcaa
     2281 cgctacgtac actcgctacg cagacgacat aatcgtgagc acgaacatga agggcgcctc
     2341 taagctgatc ttagactgct tcaagcggac catgaaggaa atcggacccg atttcaagat
     2401 caatatcaag aagttcaaaa tatgctctgc cagtggcggc tcaattgtcg tgacgggcct
     2461 taaggtctgt catgacttcc acataactct gcaccggtct atgaaggaca agatccgcct
     2521 gcacctctct ctcctgtcca aaggtattct gaaggacgag gaccacaaca agctgtccgg
     2581 gtacatcgcc tacgctaagg acatcgatcc acacttctac accaagctca ataggaagta
     2641 cttccaggag atcaagtgga tacaaaacct gcataataag gtggagccac caaagaagaa
     2701 aagaaaggtc tgatcccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg
     2761 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     2821 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     2881 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     2941 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     3001 tgccatagca gatctgcgaa atcggatcca gcggccaatt cggaacgctg acgtcatcaa
     3061 cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc
     3121 cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg
     3181 ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa
     3241 gttctgtatg agaccactga taagattccg taagagccaa acctagcatt ttatgggtta
     3301 atagcccatc gggccatgag tcatggtttc gcctagtatt ttagctattt ttttagaggg
     3361 ggtatagctc agtggtagag catttgactg cagatcaaga ggtccccggt tcaaatccgg
     3421 gtgcccccta gctatgcccg tcgttcagtt cgctgacttc tccagccctg gcctgggtca
     3481 atccttgggg cccagactga gcacgtgcta acagaggaaa ggaagccctg cttcctccag
     3541 agggcgtcgc aggacagctt ttcctagaca ggggcttcag cgaactgatc gacgtgctca
     3601 agtaggtttg gctcttacgg aatcttatca ggcccagact gagcacgtga gttttagagc
     3661 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     3721 cggtgcagag ggggtatagc tcagtggtag agcatttgac tgcagatcaa gaggtccccg
     3781 gttcaaatcc gggtgccccc tagctatgcc cgtcgttcag ttcgctgaga aagcggaagt
     3841 agggccttcg cgcacctcat ggaatccctt ctgcagcatc tagatcgctt ttccgagctt
     3901 ctggcggtct caagcactac ctacgtcagc acctgggacc ccgccacctc agcgaactga
     3961 tcgacgtgct caagtaggtt tggctcttac ggaatcttat caggaatccc ttctgcagca
     4021 ccgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
     4081 gtggcaccga gtcggtgcag agggggtata gctcagtggt agagcatttg actgcagatc
     4141 aagaggtccc cggttcaaat ccgggtgccc cctagctatg cccgtcgttc agttcgctga
     4201 acaaacggca gaagctggag gaggaagggc ctgagtccga gcagaagaaa aagttctccc
     4261 atcacatcaa ccggtggcgc attgccacga agcaggccaa tggggaggac atcgatgtca
     4321 tcagcgaact gatcgacgtg ctcaagtagg tttggctctt acggaatctt atcagagtcc
     4381 gagcagaaga agaagtttca gagctatgct ggaaacagca tagcaagttg aaataaggct
     4441 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttttt ttagcttggc
     4501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     4681 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4801 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4861 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     4921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4981 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     5041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5161 gttatccaca gaatcagggg ataacgcaag aaagaacatg tgagcaaaag gccagcaaaa
     5221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc catatgctcc gcccccctga
     5281 cgagcatcac aaaaatcgac gctcaggtca taggtggcga aacccgacag gactataaag
     5341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccaa ccctgccgct
     5401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     6301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     6361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     6421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     6481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     6541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     6601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     7021 tattg
//
