LOCUS       pAGM45244               7933 bp    DNA     circular     11-AUG-2017
DEFINITION  .
ACCESSION   urn.local...i-hjv2rfu
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|-11890814|                                                 
            VNTDBDATE|-11890814|                                               
            LSOWNER|                                                           
            VNTNAME|pAGM45244|                                                 
            VNTAUTHORNAME|Ramona Gr�tzner|                                     
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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FEATURES             Location/Qualifiers
     regulatory      26..219
                     /note="Geneious type: promoter"
                     /note="Geneious type: promoter eukaryotic"
                     /standard_name="spm\pro\(short)"
     5'UTR           220..305
                     /standard_name="TMV\omega\+"
     CDS             310..858
                     /standard_name="Bar\opt"
     regulatory      863..1209
                     /note="Geneious type: terminator"
                     /standard_name="A.t.\gcrp\ter"
     misc_feature    complement(1223..1350)
                     /standard_name="RB\short"
     misc_feature    complement(1374..2855)
                     /standard_name="RK2\trfa\(no\Esp3I)"
     misc_feature    2866..3655
                     /standard_name="pUC\ori"
     rep_origin      2901
     CDS             complement(3663..4520)
                     /standard_name="AP\r"
     rep_origin      complement(4718..5335)
     misc_feature    5365..5512
                     /note="shows similarity to T-DNA left border: GenBank
                     Accession Number J01825"
                     /standard_name="TDNA-LB"
     regulatory      5564..6905
                     /note="Geneious type: promoter"
                     /note="Geneious type: promoter eukaryotic"
                     /standard_name="35S\promoter\(no\BsaI\and\BpiI)"
     5'UTR           6922..6983
                     /standard_name="Omega\leader"
     misc_feature    6993..7655
                     /standard_name="rosea\am"
     misc_feature    7043
                     /standard_name="A>G\bpirem"
     misc_feature    7562
                     /standard_name="A>G\bpirem"
     regulatory      7666..7933
                     /note="Geneious type: terminator"
                     /standard_name="NOS\ter\+"
ORIGIN      
        1 cgctactatt gtcttccact gggtgtcaaa ggagtgtcag ttaattaaag agtgtcgggg
       61 ccgacactct taatggaagt aaaagtgtgg gttttgctgc accgacactc ttaatttaag
      121 agtgtcgggg tcccgatgaa accgacgctt ttaatttaag agtgtgggtt tttccacacc
      181 gacactctta tgaatgttac cctaaattcc ccaatcctat actgtataag agctcatttt
      241 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
      301 gatacaatgt ctcctgaaag aaggcctgct gatattagaa gggctactga ggctgatatg
      361 cctgctgtgt gcactattgt gaatcattac attgagactt ctactgtgaa tttcaggact
      421 gagcctcaag aaccacaaga gtggactgat gatcttgtga ggcttagaga gaggtatcct
      481 tggcttgttg ctgaagtgga tggtgaagtg gctggtattg cttatgctgg tccttggaag
      541 gctaggaatg cttacgattg gactgctgag tctactgttt acgtgtctcc taggcatcaa
      601 agaactggtc ttggatctac tctttacact catcttttga agtctttgga ggctcagggt
      661 ttcaagtctg tggttgctgt gattggactt cctaatgatc cttctgtgag gatgcatgag
      721 gctcttggtt atgctcctag gggaatgctt agagctgctg gtttcaaaca tggaaattgg
      781 catgatgtgg gattctggca gcttgatttc tctcttcctg ttcctcctag acctgttctt
      841 cctgtgactg agatttgagc ttactccacc taaatactat gccggttcaa ataagaaagt
      901 aatgattatg tacccaaata atacatttat aagatgtatg tgtgtgatgg acatgtttct
      961 gtatcgtacc aaaactaaat aggtggcttt tatcccaaaa aagtgctttc gtttgatgac
     1021 cttatgattt ctaaagagtg ttgttgttgc tgcttatgtt taataaaagt gatgtatatt
     1081 atcagttgat ttccccacgt ggaaggggtg attatattgc acaaaattaa gttaaaagta
     1141 acattgtggt gtaataaaaa ccctagtgaa tttataacct ataagtaagt ggtgcagtag
     1201 tgaatttatc acgaagtggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1261 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     1321 atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc aggcgctggc
     1381 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     1441 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     1501 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     1561 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     1621 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     1681 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     1741 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     1801 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     1861 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     1921 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     1981 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     2041 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     2101 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     2161 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     2221 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     2281 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga
     2341 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     2401 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     2461 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     2521 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     2581 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     2641 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     2701 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     2761 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     2821 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa
     2881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3781 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
     3841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4621 ccgcgcacga attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
     4681 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
     4741 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     4801 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     4861 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     4921 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     4981 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     5041 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     5101 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     5161 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     5221 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
     5281 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
     5341 gtcgcaaagg agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc
     5401 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     5461 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
     5521 gcagtgggcc ccactctgtg aagacaagca agaattcaag cttggaggaa ttccaatccc
     5581 acaaaaatct gagcttaaca gcacagttgc tcctctcaga gcagaatcgg gtattcaaca
     5641 ccctcatatc aactactacg ttgtgtataa cggtccacat gccggtatat acgatgactg
     5701 gggttgtaca aaggcggcaa caaacggcgt tcccggagtt gcacacaaga aatttgccac
     5761 tattacagag gcaagagcag cagctgacgc gtacacaaca agtcagcaaa cagacaggtt
     5821 gaacttcatc cccaaaggag aagctcaact caagcccaag agctttgcta aggccctaac
     5881 aagcccacca aagcaaaaag cccactggct cacgctagga accaaaaggc ccagcagtga
     5941 tccagcccca aaagagatct cctttgcccc ggagattaca atggacgatt tcctctatct
     6001 ttacgatcta ggaaggaagt tcgaaggtga aggtgacgac actatgttca ccactgataa
     6061 tgagaaggtt agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc
     6121 ctacgcagca agtctcatca agacgatcta cccgagtaac aatctccagg agatcaaata
     6181 ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaattgca tcaagaacac
     6241 agagaaagac atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt
     6301 gcttcataaa ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcctactga
     6361 atctaaggcc atgcatggag tctaagattc aaatcgagga tctaacagaa ctcgccgtca
     6421 agactggcga acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg
     6481 tcaacatggt ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag
     6541 aagatcaaag ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat
     6601 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct
     6661 acaaatgcca tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg
     6721 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca
     6781 cgtctacaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat
     6841 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     6901 cacgctcgag tataagagct catttttaca acaattacca acaacaacaa acaacaaaca
     6961 acattacaat tacatttaca attatcgata caatggaaaa gaattgtcgt ggagtgagaa
     7021 aaggtacttg gaccaaagaa gaggacactc tcttgaggca atgtatagaa gagtatggtg
     7081 aagggaaatg gcatcaagtt ccacacagag cagggttgaa ccggtgtagg aagagttgca
     7141 ggctgaggtg gttgaattat ctgaggccaa atatcaaaag aggtcggttt tcgagagatg
     7201 aagtggacct aattgtgagg cttcataagc tgttgggtaa caaatggtcg ctgattgctg
     7261 gtagaattcc tggaaggaca gctaatgacg tgaagaactt ttggaatact catgtgggga
     7321 agaatttagg cgaggatgga gaacgatgcc ggaaaaatgt tatgaacaca aaaaccatta
     7381 agctgactaa tatcgtaaga ccccgagctc ggaccttcac cggattgcac gttacttggc
     7441 cgagagaagt cggaaaaacc gatgaatttt caaatgtccg gttaacaact gatgagattc
     7501 cagattgtga gaagcaaacg caattttaca atgatgttgc gtcgccacaa gatgaagttg
     7561 aggactgcat tcagtggtgg agtaagttgc tagaaacaac ggaggatggg gaattaggaa
     7621 acctattcga ggaggcccaa caaattggaa attgagcttg atcctctaga gtcaagcaga
     7681 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     7741 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     7801 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     7861 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     7921 gttactagat cga
//
