LOCUS       sample_525              8632 bp    DNA     circular SYN 02-AUG-2024
DEFINITION  sample_525.
ACCESSION   sample_525
VERSION     sample_525
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(1959..2547)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(2564..4417)
                     /note="pLannotate"
                     /label="RHOT1"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4489..4936)
                     /note="pLannotate"
                     /label="hSyn promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     LTR             complement(6514..6771)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     LTR             complement(1119..1376)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     misc_feature    complement(5612..5845)
                     /note="pLannotate"
                     /label="RRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     polyA_signal    complement(7315..7449)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     misc_feature    complement(6342..6467)
                     /note="pLannotate"
                     /label="HIV-1 Ψ"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     misc_feature    complement(5005..5120)
                     /note="pLannotate"
                     /label="cPPT/CTS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     promoter        7497..7601
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7602..8462
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1424..1942)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="98.3"
                     /match_length="84.0"
                     /fragment="True"
                     /other="CDS"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(6819..7148)
                     /note="pLannotate"
                     /label="nef (fragment)"
                     /database="swissprot"
                     /identity="99.1"
                     /match_length="53.4"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6020..6358)
                     /note="pLannotate"
                     /label="HIV-1 gag (fragment)"
                     /database="snapgene"
                     /identity="98.8"
                     /match_length="22.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5135..5235)
                     /note="pLannotate"
                     /label="Rev (fragment)"
                     /database="snapgene"
                     /identity="99.0"
                     /match_length="28.8"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt tcatcgctgt ttcaataatg
     2581 ctttgtacat agcaaagccc aaaactgcaa aaacagtagc accaaaactt gctcgaagcc
     2641 aaaacgtgga gctcttgagg tcagcttgtg tcacgtgcgg atacatggcc attgttgtca
     2701 atttaacaaa gatatcctta ctgggggcat cagcagtatt gcaagtgaag gcttgtggtg
     2761 gaggcatttt gtgtttcctg cagaaatcag taggtgaaat actgtattct tgtttaactt
     2821 catgcaggtc tgactttgca gctacgatta agcaaggtat tctgctgtcc ataaagtgtt
     2881 gcttaaaaat cctggcacag tattcaaagg atttgggatt gctgacatca tataccaggc
     2941 atacaacatc acaaatgatt tcagcttcag ttagaaattc cgattctgag atatcatgca
     3001 acaacaagta tttctcttgt ccatatacat aaacagtgtt aatcgcatag taggatttat
     3061 gatcttcacg aattttcttc tgcctcatta agtttcttcc aagaagagcc tgaagaactc
     3121 cacttttccc acagtttttc actccaatta cattacatct gaacacattt ctttgagttt
     3181 gttttttctg caggtctatc tttttatctc ttgtcactgt aacagctgaa gcttgagact
     3241 cttgctcagt caatattgaa tagcctagat agcccaaata ttccaggcac cgctgtacat
     3301 ctaaataagt cgtgagcgtc cactgggaaa ggaatccctg gtaggttatc cagcctcttt
     3361 cattggtaca aactgtgtta ttcacatctg gcccccaagg tatgtaaggg aaaactttaa
     3421 ataaatcttt aagctcatca ggtgacaaag cacagtctct atccaaatca tgcttgtcaa
     3481 aggtgctttg gagaaataaa tatgcatgat gatttaattc agtagtgcaa tcaggaggta
     3541 ttttcagcag ggggaacaaa tattcaggtg tcaaatccag gtcatcatca taaccaaatc
     3601 gtcgaagcac agtccaagta gtttcgtgtc tccctctctg gataaaaagt gtgtgtaaaa
     3661 agagaaaacc tttcagggtc aacccactgt cagccacacc atcacttata tgttttctga
     3721 ctacattctt gacatcctcc agagcttgag gagctaatgg agtgttgaaa caaatcctct
     3781 gaaagaagtt gagttcagca tcattgagag taccatcatt atcttgatca gatattttaa
     3841 atatacgagt aagggctttt atacaagctg gtttcatctc cttctcctct gggcagtaca
     3901 ggggccctgt aggatgaaga acagctttct gtgcgtaata aaagagctct gatatgttct
     3961 tcaggttttt cgctgaacac tccacacagg tttctatttc tgtatactgg ttcataatag
     4021 gaaggatggt ctccatacta ctatattcca ccagatcaga tttgttccca accaatatta
     4081 aaggcagcct gctgtctttg tctgttcttt catttatgag aggaatccat cgacttgtta
     4141 ccttatcaat agaatgcttg ttgttaacgg catacactat acagatgaca ttagcctgag
     4201 atatttcttg atgaagttgt tcatcactct gttctgcttc tgagtaatct acaatgtgtg
     4261 ttggaactct ctctggggtg acatcagctg gaatggtgat ttcttctgcc cggggaggaa
     4321 cctcttctgg aaattcttca ctgaccagag acataatcag tgatgtcttc ccaactctag
     4381 gttctcccac cagcaggatc cgcacgtctt tcttcataga tcccaggtcc tcctcggaga
     4441 tcagcttctg ctccatggtg gcgaattcgc ccttatcgtc tagatcgact gcgctctcag
     4501 gcacgacacg actcctccgc tgcccaccgc agactgaggc agcgctgagt cgccggcgcc
     4561 gcagcgcaga tggtcgcgcc cgtgcccccc tatctcgcgc ctcgcgtggt gcggtccggc
     4621 tgggccggcg gcggcgcgga cgcgaccaag gtggccggga aggggagttt gcgggggacc
     4681 ggcgagtgac gtcagcgcgc cttcagtgct gaggcggcgg tggcgcgcgc cgccaggcgg
     4741 gggcgaaggc actgtccgcg gtgctgaagc tggcagtgcg cacgcgcctc gccgcatcct
     4801 gtttcccctc cccctctctg ataggggatg cgcaatttgg ggaatggggg ttgggtgctt
     4861 gtccagtggg tcggggtcgg tcgtcaggta ggcaccccca ccccgcctca tcctggtcct
     4921 aaaacccact tgcactcata cgcagggccc tctgcagtct agcatcgata aactggatct
     4981 ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct
     5041 ttagtttgta tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca
     5101 atcccccctt ttcttttaaa ggcgataccg tcgagatccg ttcactaatc gaatggatct
     5161 gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg
     5221 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     5281 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     5341 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     5401 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     5461 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     5521 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     5581 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     5641 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     5701 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     5761 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     5821 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     5881 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     5941 taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg
     6001 gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct
     6061 tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta
     6121 tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt
     6181 tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc
     6241 ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga
     6301 attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct
     6361 ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg
     6421 cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc
     6481 cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa
     6541 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     6601 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     6661 cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat
     6721 atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag
     6781 tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga
     6841 tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact
     6901 tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct
     6961 cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca
     7021 aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag
     7081 ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc
     7141 ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa
     7201 taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat
     7261 agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca
     7321 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     7381 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     7441 aaacaagttg atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg
     7501 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7561 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7621 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7681 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7741 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7801 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7861 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7921 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7981 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8041 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     8101 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     8161 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     8221 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     8281 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     8341 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8401 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8461 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8521 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8581 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
