LOCUS       OgeuIscB_ωRNA-v13       2747 bp ds-DNA     circular     07-MAY-2024
DEFINITION  .
ACCESSION   urn.local...1375724983699.8
VERSION     urn.local...1375724983699.8
COMMENT     Imported using the Genbank importer. File name: 823 documents from
            E coli constructs.gb
FEATURES             Location/Qualifiers
     promoter        complement(125..229)
                     /label="AmpR promoter"
                     /note="gene: bla"
     primer_bind     complement(304..323)
                     /label="pRS-marker"
                     /note="note: pRS vectors, use to sequence yeast selectable marker"
     primer_bind     328..350
                     /label="M13/pUC Forward"
                     /note="note: In lacZ gene"
     primer_bind     342..359
                     /label="M13 Forward"
                     /note="note: In lacZ gene. Also called M13-F20 or M13 (-21) Forward"
     primer_bind     343..359
                     /label="M13 fwd"
                     /note="note: common sequencing primer, one of multiple similar variants"
     misc_feature    complement(378..555)
                     /label="OgeuIscB ωRNA-v13 scaffold"
     misc_feature    complement(557..562)
                     /label="BsmBI"
     misc_feature    580..585
                     /label="BsmBI"
     promoter        complement(588..836)
                     /label="U6 promoter"
                     /note="note: RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(816..836)
                     /label="hU6-F"
                     /note="note: Human U6 promoter, forward primer"
     primer_bind     complement(850..866)
                     /label="M13 rev"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer_bind     complement(850..866)
                     /label="M13 Reverse"
                     /note="note: In lacZ gene. Also called M13-rev"
     primer_bind     complement(863..885)
                     /label="M13/pUC Reverse"
                     /note="note: In lacZ gene"
     rep_origin      complement(1252..1840)
                     /label="ori"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     complement(1332..1351)
                     /label="pBR322ori-F"
                     /note="note: pBR322 origin, forward primer"
     CDS             complement(2011..124)
                     /label="AmpR"
                     /note="gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, andrelated antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     2634..2653
                     /label="Amp-R"
                     /note="note: Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
       61 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      121 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      241 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
      301 cagtacaatc tgctctgatg ccgagctccc agtcacgacg ttgtaaaacg acggccagtg
      361 aattcgagct cggtaccaaa aaaaactctc tctgTTTCca gagaggtgat tgttccccct
      421 ggcttatctg atTTTCatca actgAcatcc gctatgcagt tccggggact ggggaaatcc
      481 cagggtgcgt ccggCctttc ggccttacga atgtctcagc ctgtgctctt tcaacctTTT
      541 Caagttggaa gagcctgaga cgaTCTCGTT CACTCTTTTc gtctctcggt gtttcgtcct
      601 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
      661 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
      721 cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattcc aattatctct
      781 ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctctggc
      841 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      901 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1021 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1141 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1681 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1921 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1981 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2041 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2101 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2161 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2221 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2281 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2341 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2401 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2461 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2521 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2581 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2641 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2701 actctcaagg atcttaccgc tgttgagatc cagttcgatg taaccca
//