LOCUS       Exported                2244 bp ds-DNA     circular SYN 09-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1375724983699.8
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Apr 9, 2024 from SnapGene Viewer 5.2.4
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 823 documents from E
            coli constructs.gb
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(187..209)
                     /label=WT067-seq
     primer_bind     585..608
                     /label=WT023
     misc_feature    complement(650..678)
                     /label=amp prom
     primer_bind     complement(721..738)
                     /label=WT811
     primer_bind     776..794
                     /label=WT1114 illumina F
     primer_bind     complement(788..804)
                     /label=WT1637
     misc_feature    805..1068
                     /label=hU6 prom
     primer_bind     1041..1065
                     /label=WT640 partial
     primer_bind     complement(1049..1076)
                     /label=HY054-USER
     primer_bind     1066..1099
                     /label=HY083
     misc_feature    1069..1099
                     /label=Cas13Rx DR 30 nt
     primer_bind     1069..1099
                     /label=WT1504
     primer_bind     1076..1095
                     /label=HY039
     primer_bind     complement(1083..1142)
                     /label=HY086-USER
     misc_feature    1100..1129
                     /label=EZH2
                     /label=EZH2 Spacer2
     primer_bind     complement(1110..1142)
                     /label=HY606
     primer_bind     1137..1154
                     /label=HY056-USER
     primer_bind     1137..1152
                     /label=WT1360
     primer_bind     complement(1139..1156)
                     /label=WT623
     misc_feature    complement(1218..1837)
                     /label=pBR322 origin
     primer_bind     complement(1233..1250)
                     /label=WT024
     primer_bind     complement(1233..1249)
                     /label=WT069-seq
     primer_bind     1800..1821
                     /label=WT070-seq
     primer_bind     complement(1801..1821)
                     /label=WT068-seq
     misc_feature    complement(join(1992..2244,1..608))
                     /label=amp marker
ORIGIN
        1 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
       61 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      121 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      181 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      241 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
      301 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      361 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      421 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      481 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      541 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      601 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      661 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      721 ccccgaaaag tgccacctga cgtcgctagc tgtacaaaaa agcaggcttt aaaggaacca
      781 attcagtcga ctggatccgg taccaaggtc gggcaggaag agggcctatt tcccatgatt
      841 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
      901 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
      961 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     1021 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccga acccctacca
     1081 actggtcggg gtttgaaacg agagcagcag caaactcctt tgctccctct ttttttaagc
     1141 ttgggccgct cgaggtacct ctctacatat gacatgtgag caaaaggcca gcaaaaggcc
     1201 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1261 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1321 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1381 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1441 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1501 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1561 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1621 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1681 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1741 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1801 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1861 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     1921 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1981 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2041 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2101 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2161 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2221 gcctccatcc agtctattaa ttgt
//
