LOCUS       pJP_Rep00        9821 bp DNA     circular SYN 01-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   UNIL
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9821
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     terminator      493..579
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     primer_bind     599..616
                     /label=pr_JP53
                     /note="color: black; sequence: 
                     agctgcaggtgtacgggttttgctgccc; added: 2023-02-09"
     CDS             complement(1117..2067)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /note="color: #993366"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2115..2337
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
                     /note="color: #ffff00"
     terminator      2829..2923
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
                     /note="color: #ffffff"
     CDS             complement(join(2953..3744,3745..3813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 2953 .. 3744 / #ccffcc
                        2: 3745 .. 3813 / #ccffcc / signal sequence
                     Cleavage site after base 3744"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3814..3918)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     terminator      4057..4143
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     CDS             complement(4170..4886)
                     /codon_start=1
                     /label=mCerulean
                     /note="color: #00ffff"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4844..4863)
                     /label=Primer 2
                     /note="color: black; sequence: cactggagttgtcccaattc; added:
                     2024-03-01"
     primer_bind     4844..4863
                     /label=Primer 1
                     /note="color: black; sequence: gaattgggacaactccagtg; added:
                     2024-03-01"
     RBS             4893..4904
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     protein_bind    4946..4962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     protein_bind    4969..4985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     CDS             5252..5974
                     /label=mKate
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     5682..5699
                     /label=pr_JP70
                     /note="color: black; sequence: aaacactcggctgggagg; added: 
                     2023-03-07"
     primer_bind     complement(6053..6069)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     terminator      6088..6174
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     CDS             complement(6232..6948)
                     /codon_start=1
                     /label=mVenus
                     /note="color: #ffff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(6906..6925)
                     /label=Primer 2
                     /note="color: black; sequence: cactggagttgtcccaattc; added:
                     2024-03-01"
     primer_bind     6906..6925
                     /label=Primer 1
                     /note="color: black; sequence: gaattgggacaactccagtg; added:
                     2024-03-01"
     RBS             6957..6968
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     promoter        complement(join(6978..7003,7004..7009,7010..7026,7027..7032,
                     7033..7051))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
                     /note="This reverse directional feature has 5 segments:
                        1: 6978 .. 7003 / #ffffff
                        2: 7004 .. 7009 / #ffffff / -10
                        3: 7010 .. 7026 / #ffffff
                        4: 7027 .. 7032 / #ffffff / -35
                        5: 7033 .. 7051 / #ffffff"
     protein_bind    7008..7026
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    7033..7051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     primer_bind     complement(7076..7103)
                     /label=pr_JP69
                     /note="color: black; sequence: 
                     GTTTTGGTCCtagacgtactcgagcacg; added: 2023-03-28"
     primer_bind     7084..7111
                     /label=pr_JP68
                     /note="color: black; sequence: 
                     agtacgtctaGGACCAAAACGAAAAAAG; added: 2023-03-28"
     misc_feature    7092..7093
                     /label=2018mar28_200bpSpacer2.5
                     /note="color: #ffcc99"
     misc_feature    7094..7154
                     /label=L3S2P21 Terminator
                     /note="color: #a6acb3"
     primer_bind     complement(7140..7176)
                     /label=pr_JP55
                     /note="color: black; sequence: 
                     CCTATAGTGAGGGTTATTAAGGCTCGGTACCAAATTC; added: 2023-02-11"
     misc_feature    7158..7180
                     /label=PT3
                     /note="color: #a6acb3; direction: RIGHT"
     protein_bind    7181..7197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     protein_bind    7204..7220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     RBS             7262..7273
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     CDS             7280..7900
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      7948..8034
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     primer_bind     8139..8166
                     /label=pr_JP58
                     /note="color: black; sequence: 
                     TAACCttaaagaggagaaaggtaccatatggtg; added: 2023-02-09"
     RBS             8141..8152
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     CDS             8170..9249
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9024..9043)
                     /label=pr_JP72
                     /note="color: black; sequence: cctgtttgatggtggttaac; added:
                     2023-03-28"
     terminator      9314..9400
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     promoter        join(9431..9449,9450..9455,9456..9472,9473..9478,
                     9479..9504)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
                     /note="This forward directional feature has 5 segments:
                        1: 9431 .. 9449 / #ffffff
                        2: 9450 .. 9455 / #ffffff / -35
                        3: 9456 .. 9472 / #ffffff
                        4: 9473 .. 9478 / #ffffff / -10
                        5: 9479 .. 9504 / #ffffff"
     protein_bind    9431..9449
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    9456..9474
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     RBS             9514..9525
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     CDS             join(9532..9821,1..421)
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /note="color: #993366"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc
       61 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc
      121 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc
      181 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc
      241 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag
      301 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa
      361 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg
      421 cgcagcaaac gacgaaaact acgctttagc agcttgatct agaggatccc atggtacgcg
      481 tgctagaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      541 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctagggt
      601 acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc tagtttgtta tcagaatcgc
      661 agatccggct tcagccggtt tgccggctga aagcgctatt tcttccagaa ttgccatgat
      721 tttttcccca cgggaggcgt cactggctcc cgtgttgtcg gcagctttga ttcgataagc
      781 agcatcgcct gtttcaggct gtctatgtgt gactgttgag ctgtaacaag ttgtctcagg
      841 tgttcaattt catgttctag ttgctttgtt ttactggttt cacctgttct attaggtgtt
      901 acatgctgtt catctgttac attgtcgatc tgttcatggt gaacagcttt gaatgcacca
      961 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
     1021 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
     1081 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     1141 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     1201 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     1261 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
     1321 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     1381 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     1441 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     1501 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     1561 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     1621 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     1681 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     1741 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     1801 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     1861 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     1921 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     1981 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     2041 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     2101 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     2161 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     2221 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     2281 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     2341 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     2401 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     2461 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     2521 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     2581 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     2641 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     2701 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     2761 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta
     2821 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2881 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgtaaac
     2941 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3001 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3061 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3121 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3181 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3241 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3301 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3361 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3421 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3481 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3541 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3601 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3661 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     3721 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     3781 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     3841 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3901 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct ctttcctgcg
     3961 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4021 cgcagcccag cctacacccc gggtctaggg cggcggattt gtcctactca ggagagcgtt
     4081 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     4141 ttgatgcctc tagactcagc taattaagct tatttgtata gttcatccat gccatgtgta
     4201 atcccagcag ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct
     4261 ttcgaaagct tagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg
     4321 ccaattggag tattttgttg ataatggtct gctagttgaa cgcctccatc ttcaatgttg
     4381 tgtctaattt tgaagttagc tttgattcca ttcttttgtt tgtctgccgt gatgtataca
     4441 ttgtctgaga tgtagttgta ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa
     4501 tcaatacctt ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca
     4561 cgtgtcttgt agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttcg
     4621 ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct tgcaaagcat
     4681 tgaacacccc aagtgagagt agtgacaagt gttggccatg gaacaggtag ttttccagta
     4741 gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg
     4801 acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca
     4861 gtgaaaagtt cttctccttt actcatggta cctttctcct ctttaatgaa ttcggtcagt
     4921 gcgtcctgct gatgtgctca gtatcttgtt atccgctcac aatgtcaatt gttatccgct
     4981 cacaattctc gaggtgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     5041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcgggtcaca aaagacattc
     5101 caccaaacgt cgtggcggct ggcgttcctt ggataaatat ctaacaccgt gcgtgttgac
     5161 tattttacct ctggcggtga taatggttgc atgtactaga atatcagcag gacgcactga
     5221 ccgaattcat taaataggag aaaggtaccg catggttagt aaaggagaag aaaataacat
     5281 ggcactgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca
     5341 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
     5401 aatcaaggcc gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
     5461 catgtacggc agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca
     5521 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
     5581 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
     5641 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
     5701 ctccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct
     5761 gaagctcgtg ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa
     5821 acccgctaag aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat
     5881 caaggaggcc gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
     5941 cgacctccct agcaaactgg ggcacagagt ctaggggatc ccccgggctg caggaattcg
     6001 atatcacgca aaaaaccccg cttcggcggg gttttttcgc gagctcgaat tcactggccg
     6061 tcgttttaca taggtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa
     6121 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     6181 ctagcacgcg tacaatggga tcccccgggc tgcaggaatt cgatatcaag cttatttgta
     6241 tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg
     6301 gtctctcttt tcgttgggat ctttcgaaag cttagattgg taggacaggt aatggttgtc
     6361 tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg
     6421 aacacctcca tcttcaatgt tgtgtctaat tttgaagttc gctttgattc cattcttttg
     6481 tttgtctgcc gtgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag
     6541 aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc
     6601 accttcaaac ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt
     6661 tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgct gtttcatatg
     6721 atctgggtat ctcgcaaagc attgaacacc ataacccaaa gtagtgacaa gtgttggcca
     6781 tggaacaggt agttttccag tagtgcaaat caatttaagg gtaagttttc cgtatgttgc
     6841 atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc
     6901 aacaagaatt gggacaactc cagtgaaaag ttcttctcct ttactcatgc ggtacctttc
     6961 tcctctttaa tgaattcggt cagtgcgtcc tgctgatgtg ctcagtatct ctatcactga
     7021 tagggatgtc aatctctatc actgataggg actcgaggtg aagacgaaag ggcctcgtgc
     7081 tcgagtacgt ctaggaccaa aacgaaaaaa ggcccccctt tcgggaggcc tcttttctgg
     7141 aatttggtac cgagccttaa taaccctcac tatagggaga ttgtgagcgg ataacaattc
     7201 gctttgtgag cggataacaa gatactgagc acatcagcag gacgcactga ccgaattcat
     7261 taaagaggag aaaggtacca tgtccagatt agataaaagt aaagtgatta acagcgcatt
     7321 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     7381 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     7441 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     7501 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     7561 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctaga
     7621 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     7681 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     7741 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     7801 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     7861 atgcggatta gaaaaacaac ttaaatgtga aagtgggtct gcagcaaacg acgaaaacta
     7921 cgctttagca gcttaatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     7981 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     8041 ccctagacct agctgcaggt cgaggataaa tatctaacac cgtgcgtgtt gactatttta
     8101 cctctggcgg tgataatggt tgcatgtact agaattcatt aaagaggaga aaggtaccat
     8161 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     8221 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     8281 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     8341 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     8401 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     8461 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     8521 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     8581 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     8641 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     8701 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     8761 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     8821 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     8881 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     8941 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     9001 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     9061 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     9121 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     9181 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     9241 agcgggcagg cagcaaacga cgaaaactac gctttagcag cttaaaagct taattagctg
     9301 agtctagagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     9361 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctagct
     9421 gcaggtcgag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga
     9481 gcacatcagc aggacgcact gaccgaattc attaaagagg agaaaggtac catgagcaca
     9541 aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat
     9601 gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg
     9661 gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc
     9721 gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga
     9781 gaaatctacg agatgtatga agcggttagt atgcagccgt c
//
