LOCUS       CROPseq-3P5P           11017 bp    DNA     circular SYN 15-NOV-2024
DEFINITION  CROPseq vector enabling gRNA detection via mRNA-based capture 
            (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 
            5'-based enrichment using a custom reverse transcription primer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Bock Lab
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2024 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(516..1131)
                     /direction=LEFT
                     /label=pUC Ori
                     /note="pUC Ori"
                     /note="/Color=#aaaaaa"
                     /note="/modified_by=User"
     LTR             1479..1891
                     /label=5' LTR
                     /note="RSV/5'LTR"
                     /note="/Color=#6bba70"
                     /note="/modified_by=User"
     misc_feature    1942..2079
                     /label=psi
                     /note="psi"
     misc_feature    2046..2410
                     /label=gag
                     /note="gag"
     misc_feature    2556..2797
                     /label=RRE
                     /note="RRE"
     misc_feature    3198..3315
                     /label=cPPT
                     /note="cPPT"
     promoter        3374..4632
                     /label=EF-1a
                     /note="EF-1a"
                     /note="/Color=#f8af3c"
                     /note="/modified_by=User"
     CDS             4645..5241
                     /label=Puro
                     /note="Puromycin resistance"
                     /note="/Color=#f2911a"
                     /note="/modified_by=User"
     misc_feature    5242..5307
                     /label=P2A
                     /label=nonstandard type: self-cleaving-linker
     misc_feature    5308..6015
                     /label=mCherry2
                     /label=nonstandard type: primer_bind_reverse
                     /note="/Color=#c72c6e"
                     /note="/Sequence=ATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAG
                     GAGTT 
                     CATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG 
                     GGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCA 
                     AGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTC 
                     CAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCC 
                     GAGGGCTTCAATTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGA 
                     CCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCAC 
                     CAACTTCCCCTCCGACGGCCCCGTAATGCAGTGTCGTACCATGGGCTGGGAGGCCTCC 
                     ACTGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGA 
                     AGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAA 
                     GCCCGTGCAGCTGCCCGGCGCCTACAACGTCGACATCAAGTTGGACATCCTTTCCCAC 
                     AACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCG 
                     GCGGCATGGACGAGCTGTACAAGTAA"
                     /note="/Transferred_From=gBlock_MS2-NLS-P2A-mCherry2-T2A"
                     /note="/Transferred_Similarity=94.49%"
                     /note="/created_by=User"
     misc_feature    5658..5665
                     /label=SbfI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=CCTGCA^GG"
                     /note="/annotation_group=CCTGCAGG"
     misc_RNA        6034..6622
                     /label=WPRE
                     /note="WPRE"
                     /note="/Color=#aaaaaa"
                     /note="/modified_by=User"
     LTR             6693..6728
                     /label=3' LTR
                     /note="3' LTR SIN"
                     /note="/Color=#6bba70"
                     /note="/modified_by=User"
     misc_feature    6729..6736
                     /label=SbfI-HF_cut
                     /label=nonstandard type: restriction
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     misc_feature    6729..6736
                     /label=SbfI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=CCTGCA^GG"
                     /note="/annotation_group=CCTGCAGG"
     misc_feature    6737..6744
                     /label=PacI_cut
                     /label=nonstandard type: restriction
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     misc_feature    6737..6744
                     /label=PacI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=TTAAT^TAA"
                     /note="/annotation_group=TTAATTAA"
     promoter        6764..7008
                     /label=hU6
                     /note="Human U6 Promoter"
                     /note="/Color=#28255a"
                     /note="/modified_by=User"
     misc_binding    7009..8892
                     /label=filler (1,885 bp)
                     /note="Filler (digest with BsmBI to replace filler with
                     sgRNA oligos)"
                     /note="/Color=#c5d7f0"
                     /note="/modified_by=User"
     misc_feature    complement(7014..7019)
                     /direction=LEFT
                     /label=BsmBI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     misc_feature    8887..8892
                     /label=BsmBI
                     /label=nonstandard type: restriction site
                     /note="/Recognition_pattern=CGTCTC(1/5)"
                     /note="/annotation_group=CGTCTC"
     misc_RNA        8894..9012
                     /label=gRNA_backbone
                     /note="/created_by=ptraxler"
     misc_feature    8960..8981
                     /label=Capture_sequence_1
                     /label=nonstandard type: capture_seq
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     terminator      9006..9012
                     /label=PolIII_termination_signal
                     /note="/Transferred_From=PT122_gRNA_cassette_v4_capseq2_gBl
                     ock_o
                     rder corrected"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     misc_feature    9032..9039
                     /label=PacI_cut
                     /label=nonstandard type: restriction
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     misc_feature    9040..9047
                     /label=SbfI-HF_cut
                     /label=nonstandard type: restriction
                     /note="/created_by=ptraxler"
                     /note="/modified_by=ptraxler"
     LTR             9048..9246
                     /label=3' LTR
                     /note="3' LTR SIN"
                     /note="/Color=#6bba70"
                     /note="/modified_by=User"
     polyA_signal    9323..9453
                     /label=SV40 polyA
                     /note="SV40 polyA"
                     /note="/Color=#aaaaaa"
                     /note="/modified_by=User"
     misc_feature    join(10601..11017,1..390)
                     /label=AmpR
                     /label=nonstandard type: primer_bind_reverse
                     /note="/Color=#bbbbbb"
                     /note="/Sequence=TTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCG
                     GTATT 
                     ATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT 
                     GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAA 
                     GAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCT 
                     GACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT 
                     GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGC 
                     GTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGA 
                     ACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT 
                     GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG 
                     GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCC 
                     CTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT 
                     AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAG 
                     TTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTA 
                     GGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTC 
                     CACTGA"
                     /note="/created_by=User"
ORIGIN
        1 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
       61 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1381 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1441 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     1501 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1561 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1621 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1681 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1741 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1861 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1921 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1981 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2041 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2101 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2161 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2221 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2281 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2341 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3121 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt gagacaaatg
     3181 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     3241 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     3301 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     3361 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3421 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3481 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     3541 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3601 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3661 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3721 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     3781 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3841 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     3901 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3961 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     4021 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     4081 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4141 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4201 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4261 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4321 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4381 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4441 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4501 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4561 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4621 caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     4861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     5041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5221 atgacccgca agcccggtgc cggatccgga gccacgaact tctctctgtt aaagcaagca
     5281 ggcgacgtgg aagaaaaccc cggtcctatg gtgagcaagg gcgaggagga caacatggcc
     5341 atcatcaagg agttcatgag gttcaaggtg cacatggagg gcagcgtgaa cggccacgag
     5401 ttcgagatcg agggcgaggg cgagggcagg ccctacgagg gcacccagac cgccaagctg
     5461 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgagccc ccagttcatg
     5521 tacggcagca aggcctacgt gaagcacccc gccgacatcc ccgactacct gaagctgagc
     5581 ttccccgagg gcttcaactg ggagagggtg atgaacttcg aggacggcgg cgtggtgacc
     5641 gtgacccagg acagcagcct gcaggacggc gagttcatct acaaggtgaa gctgaggggc
     5701 accaacttcc ccagcgacgg ccccgtgatg cagtgcagga ccatgggctg ggaggccagc
     5761 accgagagga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5821 ctgaaggacg gcggccacta cgacgccgag gtgaagacca cctacaaggc caagaagccc
     5881 gtgcagctgc ccggcgccta caacgtggac atcaagctgg acatcctgag ccacaacgag
     5941 gactacacca tcgtggagca gtacgagagg gccgagggca ggcacagcac cggcggcatg
     6001 gacgagctgt acaagtgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6601 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6721 gacaagatcc tgcaggttaa ttaataaccg ccacgccgca tctgagggcc tatttcccat
     6781 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
     6841 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     6901 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     6961 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggagacgg
     7021 ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa
     7081 tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt
     7141 tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt
     7201 tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg
     7261 cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag
     7321 cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg
     7381 aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt
     7441 tcatgatttc ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc
     7501 cagctgcttt ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta
     7561 atgcaccaat atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa
     7621 cttcgaatgc ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta
     7681 ttcctacatt tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac
     7741 aaactgttgc aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg
     7801 tagcatcttg ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta
     7861 tatattcatt tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg
     7921 aacagattca ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg
     7981 aacagcaaat catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt
     8041 atacattcca tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa
     8101 gggtgttgct ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt
     8161 aaaattcaag tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa
     8221 atagtctact aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat
     8281 agaagcttgt attccatctt tatctctagc tgagtcatca attacatatc cataactttc
     8341 ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat
     8401 tcatttaaat ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct
     8461 atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt
     8521 taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc
     8581 taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc
     8641 attcataata ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc
     8701 aggatcaaat tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg
     8761 ctcttcaacc tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt
     8821 tcctgttcca ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt
     8881 tttgtacgtc tctgtttcag agctaagctg gaaacagcat agcaagttga aataaggcta
     8941 gaccgttaac aacttggccg ctttaaggcc ggtcctagca aggccaagtg gcaccgagtc
     9001 ggtgcttttt ttcgcgggta ttggcctcag cttaattaac ctgcaggtgc tttttgcttg
     9061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9241 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     9361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     9481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     9661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     9721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     9781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     9841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    10801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    10861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    10921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    10981 actattaact ggcgaactac ttactctagc ttcccgg
//
