LOCUS       Addgene_submission:_L0- 3775 bp ds-DNA     linear       19-MAR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    41..55
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     source          41..223
                     /label="source"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    56..59
                     /label="GG4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    60..109
                     /label="Bxb1 attB modified"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    110..149
                     /label="SPACER 9"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(150..204)
                     /label="attB PhiC31"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    205..208
                     /label="GG6"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(209..223)
                     /label="BsaI linker2"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(264..3442)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(291..305)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(291..305)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(297..318)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(297..318)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(331..349)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(331..349)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(332..348)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(332..348)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(338..363)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(338..363)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(356..378)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(356..378)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(357..372)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(357..372)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     376..397
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     376..397
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     376..399
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     376..399
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(376..399)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(376..399)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(379..399)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(379..399)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3443..3775)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3630..3658)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(3630..3658)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     3639..3667
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     3639..3667
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     3647..3663
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     3647..3663
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 cgagcggccg ccagtgtgat ggatatctgc agaattcagg cactctgtgg tctcagcttt
       61 cggccggctt gtcgacgacg gccgtctccg tcgtcaggat catccgggcC TGCTTTCCTT
      121 CTGATTGAGA CGAGTAAAAC ACTGAATAGa ggtggagtac gcgcccgggg agcccaaggg
      181 cacgccctgg cacccgcacc gcggcgcttg agacctccga tcccctgaat tccagcacac
      241 tggcggccgt tactagtgga tcccctgaat tctgcagata tccatcacac tggcggccgc
      301 tcgagcatgc atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg
      361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      481 cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga
      541 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga
      601 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
      661 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
      721 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
      781 acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga
      841 tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
      901 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
      961 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     1021 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     1081 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga
     1141 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     1201 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     1261 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     1321 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     1381 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     1441 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     1501 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     1561 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     1621 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     1681 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     1741 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1801 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1861 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     1921 tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga
     1981 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat agcacgtgag gagggccacc
     2161 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     2221 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     2281 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     2341 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     2401 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     2461 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     2521 gaggagcagg actgacacgt gctaaaactt catttttaat ttaaaaggat ctaggtgaag
     2581 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     3541 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     3601 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     3661 gaccatgatt acgccaagct atttaggtga cgcgttagaa tactcaagct atgcatcaag
     3721 cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat tcagg
//