LOCUS       Addgene_submission:_L0- 4834 bp ds-DNA     circular     19-MAR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1434..1766)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1621..1649)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(1621..1649)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     1630..1658
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1630..1658
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     1638..1654
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     1638..1654
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1748..1785)
                     /label="oNL5983_GG0:pBLUNT_gibsonrev"
                     /note="sequence: ctcctgagaccacagagtgcctgaattccagcacactg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1767..1781
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1767..1811
                     /label="oNL5982_GG0:tUBQ1_gibsonfwd"
                     /note="sequence: cactctgtggtctcaggagcatttgtttgtctttaacaacggctc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1782..1785
                     /label="GG0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1786..2285)
                     /label="tUBQ1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2261..2316)
                     /label="oNL5977_NLS:tUBQ1_gibsonrev"
                     /note="sequence: ccaaagaagaagaggaaagtctaataagcttagagactcttatcaagaatcccatc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2266..2316
                     /label="oNL5976_NLS:tUBQ1_gibsonfwd"
                     /note="sequence: gattcttgataagagtctctaagcttattagactttcctcttcttctttgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2290..2295)
                     /label="Stop codon"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2296..2316)
                     /label="SV40 NLS 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2317..2352)
                     /label="GS12c"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(2353..3060)
                     /label="mNeonGreen"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 product: bright monomeric yellow-green fluorescent protein derived from LanYFP (Shaner et al., 2013) translation: MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQKAFTDVMGMDELYK* transl_table: 1"
     regulatory      2796..2805
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     primer          3057..3108
                     /label="oNL5981_NeonGreen:GG1:pBLUNT_gibsonfwd"
                     /note="sequence: ccatcattgtttactattgagaccatcaacgtcctgaattctgcagatatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(3064..3069)
                     /label="Short enhancer for gene expression"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3070..3073
                     /label="GG1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    3074..3088
                     /label="BsaI linker3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3089..1433)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(3089..3113)
                     /label="oNL5979_pBLUNT_overlap_BFP_fwd"
                     /note="sequence: gtgatggatatctgcagaattcagg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3116..3130)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3116..3130)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3122..3143)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(3122..3143)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3156..3174)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3156..3174)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3157..3173)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(3157..3173)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3163..3188)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(3163..3188)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3181..3203)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(3181..3203)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(3182..3197)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3182..3197)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     3201..3222
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     3201..3222
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     3201..3224
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     3201..3224
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3201..3224)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(3201..3224)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3204..3224)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3204..3224)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
       61 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      121 gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg ccgttccggt
      181 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
      241 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
      301 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
      361 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
      421 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
      481 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctaaaact
      541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
      601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
      661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
      721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
      781 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
      841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
      901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
      961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1021 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     1441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1501 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1561 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1621 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg
     1681 acgcgttaga atactcaagc tatgcatcaa gcttggtacc gagctcggat ccactagtaa
     1741 cggccgccag tgtgctggaa ttcaggcact ctgtggtctc aggagcattt gtttgtcttt
     1801 aacaacggct cacaacctgt gaccgtagag agcctcaatg catggaacat gcagaagcct
     1861 ctgaagatga accaaggtgc aaagtgatca catcattctt taattttttc gttgacaaat
     1921 ttaattcatt cgttaaaaca gagagtctgt cacaatatat tgtagaaaaa aggaacctat
     1981 tgtgtttctt ttacaatcaa tacaatttgt acatagttta tcactactaa caatgtattg
     2041 atcttatatt ttattaatga aacacgtctc ctaatcacat aaacggtcat tatttcacga
     2101 tacttgtata gattggaatt aagcaagaat cttccgtaat cgattcagaa taatagaaaa
     2161 ccaaatcctt ttactgacat tatcttttaa catagaaagt cactgaaaca agaaaaacaa
     2221 accctatcaa agggaagaca acaaaaaaaa gcaagcaaga gatgggattc ttgataagag
     2281 tctctaagct tattagactt tcctcttctt ctttggagag ccagatccag atcctgatcc
     2341 tgagccactg cccttgtaca gctcgtccat gcccatcaca tcggtaaagg ccttttgcca
     2401 ctccttgaag ttgagctcgg tcttggagtg cttgagctcc gtcttacgga acacgtacat
     2461 cggctggttc ttcagatagt tagccgccat tggcttggca aaggtgtagg tggtccgcgc
     2521 agtgctccgg tagcgcttgc catttccagt ggtgtaactc cacttaaagg tactgatgat
     2581 ggttttgtcg ttggggtaag tcttcttcga cctgcaccag tccgcagcgg tcagcgagtt
     2641 ggtcatcaca ggaccgtcag cagggaaacc agtccccttc acctgggcct ctcctttgat
     2701 gtggcttccc tcgtaggtgt agcggtagtt aacagtaagg gaggcaccat cttcaaactg
     2761 cattgtgcga tggacttggt agccggagcc atctaccatg gcggcctgga aaggcgacat
     2821 cccgtcaggg tagggcaggt actgatggaa gccatacccg atatgaggga ccagaatcca
     2881 gggggagaac tggaggtcac ccttggtgga cttcaggttt aactcctcat aaccatcatt
     2941 tggattgccg gtgccctgac ccaccatgtc aaagtccaca ccgttgatgg agccaaagat
     3001 gtgtaactca tgtgtcgctg ggagagaggc catgttatcc tcctcgccct tgctcaccat
     3061 cattgtttac tattgagacc atcaacgtcc tgaattctgc agatatccat cacactggcg
     3121 gccgctcgag catgcatcta gagggcccaa ttcgccctat agtgagtcgt attacaattc
     3181 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3241 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3301 cccttcccaa cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag
     3361 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg
     3421 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     3481 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     3541 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     3601 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggcatga gattatcaaa
     3661 aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc
     3721 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     3781 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     3841 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     3901 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca
     3961 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4021 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4081 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4141 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4201 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4261 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4321 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4381 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4441 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4501 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4561 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4621 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4681 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4741 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attattaacg cttacaattt
     4801 cctgatgcgg tattttctcc ttacgcatct gtgc
//