LOCUS       Addgene_submission:_L0I 5099 bp ds-DNA     linear       19-MAR-2024
DEFINITION  .
KEYWORDS    "ID" "File Name" "Description" "GoldenGate" "Part/Tool"
            "Resistance" "Backbone"
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1..19
                     /label="source:Synthetic"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1..42
                     /label="pSPCHfwd_SG"
                     /note="sequence: cactctgtggtctcaggagCGTTACTAAAAAGACATGGTCAG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    16..19
                     /label="GG0"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    20..1568
                     /label="Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          61..101
                     /label="SPCH F"
                     /note="sequence: cactctgtggtctcaggagCCTAACGACTACCTGGTTTGTC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1351..1391)
                     /label="SPCH R"
                     /note="sequence: acgttgatggtctcacattCGAGGACTAAACTATCGCTATC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1545..1587)
                     /label="pSPCHrev_SG"
                     /note="sequence: acgttgatggtctcacattCGTGATTAGAGATATATCCTtctc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1569..1570
                     /label="GG1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1569..1587
                     /label="source:Synthetic"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1573..1587)
                     /label="BsaI linker3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1588..4766)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1615..1629)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1615..1629)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1621..1642)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1621..1642)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1655..1673)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1655..1673)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1656..1672)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(1656..1672)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1662..1687)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1662..1687)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1680..1702)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1680..1702)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1681..1696)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1681..1696)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     1700..1721
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     1700..1721
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     1700..1723
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     1700..1723
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1700..1723)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1700..1723)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(1703..1723)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1703..1723)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4767..5099)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4954..4982)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(4954..4982)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     4963..4991
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     4963..4991
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4971..4987
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     4971..4987
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 cactctgtgg tctcaggagC GTTACTAAAA AGACATGGTC AGGTAAATAA TATCTGACAG
       61 CCAACTCATT AACTTCAAAC CTAACGACTA CCTGGTTTGT CTTGGCTCCA TCTTCTTAAA
      121 TTTTCGTTTA TTTTCTAAAA CATTTGAATG GATTATCAAA AAAGAAAATG TCAAGCAATA
      181 TTTATATTTA TGTACAAATA TTTCAAAAAC TTTTGAAAGG GAAAGTTCAA ATGAAGGGTG
      241 GCTCCTCAAG ATGCAATTTG ATTTTGATGA GACAAAATGA GGCACGTCTA GTATGACATC
      301 AACTATTTAT TGCCTTAGAT CTATCCCTTG TCTTTTGTTT TCTTGAAAAC AAATTCGTTT
      361 GCTCCCTGTA TCAATATAAA GATTAACTTA CAAAAGAGGT Gatatatata tatatatata
      421 CACATTATAT CCCATCTTAT TAATAATGCT TACTAATATT GCTAAATTCA TATTCTATTT
      481 TGGGTTGGTT TTATAAGAAA TTGAATTCTT ATGCCATCTC ATCAGGTGTT TATGAAATAT
      541 GTTAGGGATC CAGTTGAACT GAACTAACAT TACTAAATTG AATTAATTAA ATTGTATGTA
      601 TGGCTCGGGA AAGAAGAAGG AACGGATTTT CTTTCAGAAT TGTGGAATCG AATTTTGTTC
      661 TTCTTTTTGG GCTTAAATGT TGGTTAGATT ACACCATGAC ATAAACAAAG GCCAAAGTAT
      721 ATGCTTTAAG TGAAAATCTG TATTTTTAAA TCGACTGGCG ATAACATGTC TGAAATTGGT
      781 CTATCAATCA TAAAAAGTCT CTAAATATGT ATCtttttat tttttGAATA ATGAATGATC
      841 TATAATGTGA ATGTACAACA CATCATTCAA TTGATTGTCT CACGATTGAG GCGCTAAAAT
      901 AAATGTGAAC TCACTAATTT CAAATGtttt ttttttgttt gtttttttGt atatatatat
      961 aCTTGGAAAC CGGGATTGGT CGGACCATAT TCGCAACCCG AACATATACG TAAATTAAAT
     1021 ACAAAGAACG TACATGTCCG TGTTTATGTG TATTACATGT TTGTGTGTAC AAAAAACAAT
     1081 TATACCGAGC TTGTGATTAT TCAAAATTTT ACCCCCATAA AAATCTTTTC CTAAGGAATT
     1141 TATGTATCTT TCCCTTTGCA ATATGCAACC TTTATCTCTG TAATATAAAG TATACAATAA
     1201 GACGTACCat atatatatat GTAGATGCAT ATTTATATCT AAAACATAAA TGAGACACGA
     1261 AAAACCTAGA TCCTCCCCCA AATTTCATCC AACGGCACAA ACCGCATAAT AAATAACCCT
     1321 ATCAAAATAT AATAATAAAA ATTGAAATTT GATAGCGATA GTTTAGTCCT CGATGAATGC
     1381 GAGTCCCTCA TATTTACTCC TTTTGGCATT CACTCTCTTT ATTTCTCTTT CTCAACTTGA
     1441 TATGTCTTTT GGCGTCTCTT TCTTTCCCTA AATCTTTCTT TTATTTTGCT GTTTaaaaaa
     1501 aaaaTCCAAC CATAAGACAA AACAACGAAC GAGGAagaga gagagagaAG GATATATCTC
     1561 TAATCACGaa tgtgagacca tcaacgtcct gaattctgca gatatccatc acactggcgg
     1621 ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca
     1681 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1741 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1801 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga
     1861 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga
     1921 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt
     1981 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt
     2041 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc
     2101 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa
     2161 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc
     2221 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     2281 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     2341 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     2401 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa
     2461 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     2521 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     2581 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     2641 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
     2701 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     2761 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     2821 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     2881 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     2941 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     3001 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     3061 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3121 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3181 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     3241 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc
     3301 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac
     3361 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3421 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc
     3481 caccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     3541 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     3601 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     3661 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     3721 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     3781 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     3841 ggccgaggag caggactgac acgtgctaaa acttcatttt taatttaaaa ggatctaggt
     3901 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3961 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4021 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4081 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4141 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4201 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4261 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4321 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4381 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4441 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4501 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4561 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4621 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4681 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4741 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4801 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4861 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4921 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4981 ctatgaccat gattacgcca agctatttag gtgacgcgtt agaatactca agctatgcat
     5041 caagcttggt accgagctcg gatccactag taacggccgc cagtgtgctg gaattcagg
//