LOCUS       Addgene_submission:_L0I 7425 bp ds-DNA     circular     19-MAR-2024
DEFINITION  .
KEYWORDS    "ID" "File Name" "Description" "GoldenGate" "Part/Tool"
            "Resistance" "Backbone"
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label="BsaI linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     source          1..19
                     /label="source:Synthetic"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    16..19
                     /label="GG0"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    20..1568
                     /label="SPCH Promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1569..1570
                     /label="GG1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1573..3384
                     /label="PhiC31"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3385..3390
                     /label="Stop codon"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3395..3894
                     /label="tUBQ1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3869..3894)
                     /label="tUBQ1_GG4 rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: cgtgatgaggtctcaaagccatttgtttgtctttaacaacggctc"
     misc_feature    3895..3898
                     /label="GG4"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(3899..3913)
                     /label="BsaI linker4"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(3914..7092)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3941..3955)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3941..3955)
                     /label=">pNL_oligo_2447"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(3947..3968)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3947..3968)
                     /label=">pNL_oligo_1301"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(3981..3999)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3981..3999)
                     /label=">pNL_oligo_3104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3982..3998)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(3982..3998)
                     /label=">pNL_oligo_16"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3988..4013)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(3988..4013)
                     /label=">pNL_oligo_1548"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4006..4028)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4006..4028)
                     /label=">pNL_oligo_552"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(4007..4022)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4007..4022)
                     /label=">pNL_oligo_37"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     4026..4047
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     4026..4047
                     /label=">pNL_oligo_3070"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     4026..4049
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     4026..4049
                     /label=">pNL_oligo_2216"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(4026..4049)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(4026..4049)
                     /label=">pNL_oligo_2215"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(4029..4049)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4029..4049)
                     /label=">pNL_oligo_2699"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(7093..7425)
                     /label="Digestion of pBlunt_BB (reversed)(concatenatedsequence 1)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(7280..7308)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(7280..7308)
                     /label=">pNL_oligo_2085"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     7289..7317
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     7289..7317
                     /label=">pNL_oligo_553"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7297..7313
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     7297..7313
                     /label=">pNL_oligo_38"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 cactctgtgg tctcaggagC GTTACTAAAA AGACATGGTC AGGTAAATAA TATCTGACAG
       61 CCAACTCATT AACTTCAAAC CTAACGACTA CCTGGTTTGT CTTGGCTCCA TCTTCTTAAA
      121 TTTTCGTTTA TTTTCTAAAA CATTTGAATG GATTATCAAA AAAGAAAATG TCAAGCAATA
      181 TTTATATTTA TGTACAAATA TTTCAAAAAC TTTTGAAAGG GAAAGTTCAA ATGAAGGGTG
      241 GCTCCTCAAG ATGCAATTTG ATTTTGATGA GACAAAATGA GGCACGTCTA GTATGACATC
      301 AACTATTTAT TGCCTTAGAT CTATCCCTTG TCTTTTGTTT TCTTGAAAAC AAATTCGTTT
      361 GCTCCCTGTA TCAATATAAA GATTAACTTA CAAAAGAGGT Gatatatata tatatatata
      421 CACATTATAT CCCATCTTAT TAATAATGCT TACTAATATT GCTAAATTCA TATTCTATTT
      481 TGGGTTGGTT TTATAAGAAA TTGAATTCTT ATGCCATCTC ATCAGGTGTT TATGAAATAT
      541 GTTAGGGATC CAGTTGAACT GAACTAACAT TACTAAATTG AATTAATTAA ATTGTATGTA
      601 TGGCTCGGGA AAGAAGAAGG AACGGATTTT CTTTCAGAAT TGTGGAATCG AATTTTGTTC
      661 TTCTTTTTGG GCTTAAATGT TGGTTAGATT ACACCATGAC ATAAACAAAG GCCAAAGTAT
      721 ATGCTTTAAG TGAAAATCTG TATTTTTAAA TCGACTGGCG ATAACATGTC TGAAATTGGT
      781 CTATCAATCA TAAAAAGTCT CTAAATATGT ATCtttttat tttttGAATA ATGAATGATC
      841 TATAATGTGA ATGTACAACA CATCATTCAA TTGATTGTCT CACGATTGAG GCGCTAAAAT
      901 AAATGTGAAC TCACTAATTT CAAATGtttt ttttttgttt gtttttttGt atatatatat
      961 aCTTGGAAAC CGGGATTGGT CGGACCATAT TCGCAACCCG AACATATACG TAAATTAAAT
     1021 ACAAAGAACG TACATGTCCG TGTTTATGTG TATTACATGT TTGTGTGTAC AAAAAACAAT
     1081 TATACCGAGC TTGTGATTAT TCAAAATTTT ACCCCCATAA AAATCTTTTC CTAAGGAATT
     1141 TATGTATCTT TCCCTTTGCA ATATGCAACC TTTATCTCTG TAATATAAAG TATACAATAA
     1201 GACGTACCat atatatatat GTAGATGCAT ATTTATATCT AAAACATAAA TGAGACACGA
     1261 AAAACCTAGA TCCTCCCCCA AATTTCATCC AACGGCACAA ACCGCATAAT AAATAACCCT
     1321 ATCAAAATAT AATAATAAAA ATTGAAATTT GATAGCGATA GTTTAGTCCT CGATGAATGC
     1381 GAGTCCCTCA TATTTACTCC TTTTGGCATT CACTCTCTTT ATTTCTCTTT CTCAACTTGA
     1441 TATGTCTTTT GGCGTCTCTT TCTTTCCCTA AATCTTTCTT TTATTTTGCT GTTTaaaaaa
     1501 aaaaTCCAAC CATAAGACAA AACAACGAAC GAGGAagaga gagagagaAG GATATATCTC
     1561 TAATCACGaa tggacacgta tgccggcgct tatgatagac aatcaagaga aagagagaac
     1621 tcttctgccg cctctccagc tacacagaga tctgccaacg aggataaagc tgctgattta
     1681 caaagagagg tagagagaga tgggggtagg tttagatttg tgggtcattt ctctgaggct
     1741 cctggtacat cagcctttgg tactgctgaa cggcctgaat ttgaaagaat attaaatgag
     1801 tgtcgggccg gtcgactaaa tatgataata gtttatgatg tatctaggtt cagtagactc
     1861 aaagttatgg atgcaattcc gatcgtgagc gagctacttg cactcggtgt tactatcgta
     1921 agtacacaag aaggtgtatt caggcagggt aatgtgatgg accttataca ccttattatg
     1981 cgactcgacg ctagccataa agagtcatca ctgaagagtg ctaaaatctt ggacaccaag
     2041 aacctgcaaa gggagcttgg aggctatgta ggaggaaaag ctccgtatgg tttcgaactc
     2101 gtgagtgaga caaaagagat taccagaaat ggtaggatgg tgaacgtggt tattaataag
     2161 ctagctcata gcacaacccc tctcacaggg ccttttgagt tcgaacctga cgttattaga
     2221 tggtggtgga gagagatcaa aactcacaaa catcttccat ttaagccagg tagccaggct
     2281 gctatccacc ctggatctat cactgggctt tgtaaaagga tggatgctga cgcagtgcca
     2341 actcgagggg aaacaatcgg taagaagact gcatcttccg cttgggatcc cgctacagtt
     2401 atgagaatcc ttcgggatcc gcgtattgct ggattcgccg cagaagtcat ttataagaaa
     2461 aaacctgatg gtactcctac tacaaaaatc gaaggttata ggatccaaag ggatcctatt
     2521 actttgcgtc cagttgaatt agactgtgga ccgatcatcg aacctgccga gtggtacgag
     2581 ttgcaggcct ggcttgatgg tagagggcgt ggaaagggtt tatctcgggg acaggcaatt
     2641 ttgtcagcaa tggataagct ttattgtgag tgtggggctg ttatgacatc aaaacgtgga
     2701 gaagagtcaa tcaaggattc ttacaggtgc agacgtcgta aagtggtgga cccttctgct
     2761 ccaggacagc atgagggtac atgcaacgtt tctatggctg ctcttgataa atttgtggcc
     2821 gaacgtattt tcaacaagat tagacacgct gaaggggatg aggaaacttt agctcttctt
     2881 tgggaggctg cccggcgttt tggtaagcta acagaggcac cagaaaaaag tggcgaaaga
     2941 gctaatttgg tagccgaaag agcagacgcc ttgaatgcat tggaagaact ttacgaagat
     3001 agggctgccg gagcatatga tggaccagtt ggtcggaagc atttcaggaa gcaacaagct
     3061 gcactaactt tgagacagca aggcgcagag gagcgactcg cagagcttga ggccgccgag
     3121 gccccaaagc ttccactcga tcaatggttt cctgaggatg ccgacgctga tcctacaggt
     3181 ccaaagagct ggtggggaag agctagcgta gacgataaga gagtgtttgt gggtctattt
     3241 gttgacaaga ttgtagttac taagtccact acgggtcgag gacagggcac tcccatcgaa
     3301 aagagagcca gtattacctg ggcaaagcct ccaaccgatg atgatgaaga cgatgcacaa
     3361 gatggtaccg aggatgtggc agcataataa gcttagagac tcttatcaag aatcccatct
     3421 cttgcttgct tttttttgtt gtcttccctt tgatagggtt tgtttttctt gtttcagtga
     3481 ctttctatgt taaaagataa tgtcagtaaa aggatttggt tttctattat tctgaatcga
     3541 ttacggaaga ttcttgctta attccaatct atacaagtat cgtgaaataa tgaccgttta
     3601 tgtgattagg agacgtgttt cattaataaa atataagatc aatacattgt tagtagtgat
     3661 aaactatgta caaattgtat tgattgtaaa agaaacacaa taggttcctt ttttctacaa
     3721 tatattgtga cagactctct gttttaacga atgaattaaa tttgtcaacg aaaaaattaa
     3781 agaatgatgt gatcactttg caccttggtt catcttcaga ggcttctgca tgttccatgc
     3841 attgaggctc tctacggtca caggttgtga gccgttgtta aagacaaaca aatggctttg
     3901 agacctcatc acgcctgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
     3961 atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
     4021 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4081 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4141 gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     4201 tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc
     4261 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     4321 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     4381 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     4441 cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta
     4501 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     4561 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     4621 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     4681 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     4741 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg
     4801 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4861 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4921 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4981 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5041 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5101 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5161 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5221 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5281 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     5341 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5401 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5461 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5521 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5581 tcgccttctt gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt
     5641 tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg
     5701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5761 gacaataacc ctgataaatg cttcaataat agcacgtgag gagggccacc atggccaagt
     5821 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     5881 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     5941 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     6001 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     6061 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     6121 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     6181 actgacacgt gctaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7321 acgccaagct atttaggtga cgcgttagaa tactcaagct atgcatcaag cttggtaccg
     7381 agctcggatc cactagtaac ggccgccagt gtgctggaat tcagg
//