LOCUS       Exported                5784 bp ds-DNA     linear   SYN 10-APR-2024
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..76
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     misc_feature    79..662
                     /label=Chick Beta Actin Protomer
                     /note="Chick Beta Actin Protomer"
     misc_feature    1747..1761
                     /label=Insertion Point 1
                     /note="Insertion Point 1"
     misc_feature    1750..1755
                     /label=Kpni
     misc_feature    1762..3045
                     /label=Ordered Fragment
                     /note="Ordered Fragment"
     CDS             1762..1815
                     /codon_start=1
                     /label=HSA Signal Sequence
                     /note="HSA Signal Sequence (Different than PTPalpha Signal 
                     Sequence in original vector)"
                     /translation="MKWVTFISLLFLFSSAYS"
     CDS             1816..2997
                     /codon_start=1
                     /label=DENV2 sRecE
                     /note="DENV2 sRecE"
                     /translation="MRCIGMSNRDFVEGVSGGSWVDIVLEHGSCVTTMAKNKPTLDFEL
                     IKTEAKQPATLRKYCIEAKLTNTTTESRCPTQGEPSLNEEQDKRFVCKHSMVDRGWGNG
                     CGLFGKGGIVTCAMFRCKKNMEGKVVQPENLEYTIVITPHSGEEHAVGNDTGKHGKEIK
                     ITPQSSITEAELTGYGTVTMECSPRTGLDFNEMVLLQMENKAWLVHRQWFLDLPLPWLP
                     GADTQGSNWIQKETLVTFKNPHAKKQDVVVLGSQEGWMHRALTGATEIQMSSGNLLWPG
                     HLKCRLRMDKLQLKGMSYSMCTGKFKVVKEIAETQHGTIVIRVQYEGDGSPCKIPFEIM
                     DLEKRHVLGRLITVNPIVTEKDSPVNIEAEPPFGDSYIIIGVEPGQLKLNWFKK"
     misc_feature    2590..2601
                     /label=IntFc2
                     /note="IntFc2"
     CDS             2590..2592
                     /codon_start=1
                     /label=A259W
                     /note="A259W"
                     /translation="W"
     CDS             2599..2601
                     /codon_start=1
                     /label=T262R
                     /note="T262R"
                     /translation="R"
     misc_feature    2650..2655
                     /label=UndPk6
                     /note="UndPk6"
     CDS             2650..2652
                     /codon_start=1
                     /label=F279W
                     /note="F279W"
                     /translation="W"
     CDS             2653..2655
                     /codon_start=1
                     /label=T280P
                     /note="T280P"
                     /translation="P"
     CDS             2998..3015
                     /codon_start=1
                     /label=GS Linker 1
                     /note="GS Linker 1"
                     /translation="GSSGGS"
     CDS             3016..3039
                     /codon_start=1
                     /label=8x His Tag
                     /note="8x His Tag"
                     /translation="HHHHHHHH"
     misc_feature    3046..3069
                     /label=Insertion Point 2
                     /note="Insertion Point 2"
     misc_feature    3206..3261
                     /label=Beta-Globulin Terminator
                     /note="Beta-Globulin Terminator"
     CDS             complement(3610..3624)
                     /codon_start=1
                     /label=lacZa part A
                     /translation="MTMIT"
     regulatory      complement(3642..3662)
                     /label=lac operator
                     /note="Lac repressor binding site"
     misc_feature    3644..3659
                     /label=Lac operator
                     /note="Lac operator"
     misc_feature    complement(3668..3698)
                     /label=Lac Promoter
                     /note="Lac Promoter"
     regulatory      complement(3668..3698)
                     /label=lac promoter
                     /note="lac promoter"
     regulatory      complement(3709..3733)
                     /label=CAP binding site
                     /note="CAP binding site"
     misc_feature    3713..3734
                     /label=CAP Binding Site
                     /note="CAP Binding Site"
     misc_feature    3791..3796
                     /label=Untitled
     rep_origin      complement(4024..4645)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="C171T is a pUC mutation; none of the other observed 
                     variants are described in the literature"
     misc_feature    4044..4622
                     /label=pUMB1 Ori (from pBR322)
                     /note="pUMB1 Ori (from pBR322)"
     CDS             complement(4793..5653)
                     /codon_start=1
                     /label=AmpR Beta-Lactamase
                     /note="AmpR Beta-Lactamase "
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(5688..5723)
                     /label=AmpR promoter
                     /note="includes -35, -10, and transcription start site"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag
     1741 gccgagttcg gtaccgccac catgaagtgg gtaaccttta tttcccttct ttttctcttt
     1801 agctcggctt attccatgag gtgtatcggc atgtccaaca gggactttgt ggagggagtg
     1861 agcggcggca gctgggtgga cattgtgctg gagcatggaa gctgcgtgac cacgatggcg
     1921 aaaaacaagc ccaccctgga cttcgagctc atcaagacag aggctaaaca gcccgccacc
     1981 ctcaggaagt actgcatcga ggccaagctg accaacacaa caaccgagtc cagatgccct
     2041 acacagggcg aacccagcct caacgaagag caggacaaga ggttcgtgtg caaacacagc
     2101 atggtggaca ggggctgggg caatggatgc ggactcttcg gaaaaggcgg catcgtgacc
     2161 tgcgccatgt tcaggtgtaa aaagaacatg gaaggcaagg tggtgcagcc cgaaaatctg
     2221 gagtatacca tcgtgattac cccccacagc ggagaggagc acgccgtggg caatgacacc
     2281 ggcaagcacg gcaaagagat taagatcacc ccccagtcct ccattaccga agctgaactg
     2341 acaggctacg gcaccgtgac aatggagtgt agccccagga ccggactgga tttcaacgag
     2401 atggtcctgc tacagatgga gaacaaggcc tggctcgtgc acaggcaatg gtttctggat
     2461 ctgcctctgc cttggctgcc tggcgccgat acacagggct ccaactggat acagaaagag
     2521 accctcgtca ccttcaagaa tccccatgct aagaagcagg acgtggtggt gctgggcagc
     2581 caagaaggct ggatgcaccg ggccctgacc ggagctaccg agatccagat gagctccggc
     2641 aacctgctgt ggcccggcca tctgaaatgt aggctgagga tggataagct gcaactcaaa
     2701 ggcatgtcct actccatgtg caccggaaag ttcaaggtgg tgaaagaaat cgccgaaaca
     2761 cagcacggca ccatcgtgat cagggtgcag tatgagggcg acggctcccc ctgtaagatc
     2821 cccttcgaaa tcatggacct ggaaaagagg cacgtgctgg gcaggctcat caccgtgaac
     2881 cccattgtca cagagaaaga ctcccccgtg aacatcgagg ccgagcctcc ctttggcgac
     2941 tcctacatca tcattggcgt ggagcccgga cagctcaagc tgaactggtt caagaagggc
     3001 agcagcggcg gcagccatca ccaccatcat caccatcatt agtaatgact cgagcgataa
     3061 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     3121 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     3181 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     3241 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     3301 cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca
     3361 aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     3421 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     3481 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     3541 ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc
     3601 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3781 gccagcggat cgatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3841 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3901 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3961 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4021 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4081 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4141 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4201 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     4261 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4321 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4381 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4441 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4501 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4561 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5761 catttccccg aaaagtgcca cctg
//
