LOCUS       Exported                5814 bp ds-DNA     linear   SYN 10-APR-2024
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Apr 10, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    2..78
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     misc_feature    79..662
                     /label=Chick Beta Actin Protomer
                     /note="Chick Beta Actin Protomer"
     misc_feature    1744..1761
                     /label=Insertion Point 1
                     /note="Insertion Point 1"
     CDS             1762..1815
                     /codon_start=1
                     /label=HSA Signal Sequence
                     /note="HSA Signal Sequence (Different than PTPalpha Signal 
                     Sequence in original vector)"
                     /translation="MKWVTFISLLFLFSSAYS"
     CDS             1816..3027
                     /codon_start=1
                     /label=ZIKV sRecE IntFc8
                     /note="ZIKV sRecE IntFc8"
                     /translation="IRCIGVSNRDFVEGMSGGTWVDIVLEHGKCVTVMMQDKPTVDIEL
                     VTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGNG
                     CDLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDTGHETDEN
                     RAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIP
                     LPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGWVHRALAGALEAEMDGAK
                     GRLWPGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCK
                     VPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHH
                     WHRSG"
     CDS             1900..1902
                     /codon_start=1
                     /label=S29K
                     /note="S29K"
                     /translation="K"
     CDS             1918..1920
                     /codon_start=1
                     /label=A35M
                     /note="A35M"
                     /translation="M"
     CDS             2131..2133
                     /codon_start=1
                     /label=G106D
                     /note="G106D"
                     /translation="D"
     CDS             2605..2607
                     /codon_start=1
                     /label=A259W
                     /note="A259W"
                     /translation="W"
     CDS             2614..2616
                     /codon_start=1
                     /label=T262R
                     /note="T262R"
                     /translation="R"
     misc_feature    2668..2673
                     /label=UndPk6
                     /note="UndPk6"
     CDS             2668..2670
                     /codon_start=1
                     /label=S285W
                     /note="S285W"
                     /translation="W"
     CDS             2671..2673
                     /codon_start=1
                     /label=S280P
                     /note="S280P"
                     /translation="P"
     CDS             3028..3045
                     /codon_start=1
                     /label=GS Linker 1
                     /note="GS Linker 1"
                     /translation="GSSGGS"
     CDS             3046..3069
                     /codon_start=1
                     /label=8x His Tag
                     /note="8x His Tag"
                     /translation="HHHHHHHH"
     misc_feature    3076..3093
                     /label=Insertion Point 2
                     /note="Insertion Point 2"
     regulatory      3236..3291
                     /label=RBG polyA signal
                     /note="rabbit beta-globin polyadenylation signal"
     CDS             complement(3640..3654)
                     /codon_start=1
                     /label=lacZa part A
                     /translation="MTMIT"
     regulatory      complement(3672..3692)
                     /label=lac operator
                     /note="Lac repressor binding site"
     regulatory      complement(3698..3728)
                     /label=lac promoter
                     /note="lac promoter"
     regulatory      complement(3739..3763)
                     /label=CAP binding site
                     /note="CAP binding site"
     rep_origin      complement(4054..4675)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="C171T is a pUC mutation; none of the other observed 
                     variants are described in the literature"
     CDS             complement(4823..5683)
                     /codon_start=1
                     /label=AmpR Beta-Lactamase
                     /note="AmpR Beta-Lactamase "
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     regulatory      complement(5718..5753)
                     /label=AmpR promoter
                     /note="includes -35, -10, and transcription start site"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattgcg gccgtctcag
     1741 gccgagttcg gtaccgccac catgaagtgg gtaaccttta tttcccttct ttttctcttt
     1801 agctcggctt attccatccg ctgcattggt gtgtcaaatc gggatttcgt agagggaatg
     1861 tctgggggca catgggttga cattgtcctc gaacatggca agtgcgtcac agtgatgatg
     1921 caggataaac cgacggtaga catcgagctt gttactacca ctgtgtccaa tatggcggaa
     1981 gtgagaagct attgttatga ggcgagcatc agtgatatgg cgagtgattc aaggtgtccc
     2041 acccaaggcg aggcgtactt ggataaacag agtgatacac aatacgtgtg taagcgaaca
     2101 ctcgttgaca ggggttgggg gaacgggtgc gacctcttcg gtaagggaag tttggtgaca
     2161 tgtgcgaagt ttgcctgctc taagaaaatg actgggaagt ccatacaacc agaaaatctt
     2221 gaatatcgaa ttatgttgtc cgttcacggt agccagcatt ctggaatgat tgtcaatgat
     2281 actgggcacg aaactgatga gaatcgagcg aaagtagaga tcacgcccaa ctcacctcgc
     2341 gcggaagcga cgttgggagg gtttggttcc ctgggactgg attgcgagcc tagaactgga
     2401 ctcgactttt ccgacctgta ctatttgaca atgaataata agcattggtt ggtccacaag
     2461 gagtggttcc atgatattcc gctgccctgg catgcgggcg cggacactgg aactccgcat
     2521 tggaataaca aagaagccct cgttgagttt aaagatgccc acgccaagag gcaaacagtt
     2581 gtcgtattgg gctcccagga aggttgggtg caccgggctt tggccggagc cctcgaagca
     2641 gaaatggacg gagcgaaagg tcgcctttgg cccggacacc tcaagtgcag gctgaagatg
     2701 gacaaactcc gactcaaagg ggtttcctat agcctttgca cggcagcctt tacattcaca
     2761 aaaatccccg ccgaaacgtt gcacggaact gtgaccgtgg aggtgcagta tgccggcacg
     2821 gacggccctt gcaaagtccc ggctcaaatg gctgtagata tgcaaacatt gaccccggtc
     2881 ggacggctca ttactgccaa tcctgttatc acagaatcca cagaaaactc aaagatgatg
     2941 cttgagctcg accctccctt cggagactca tatattgtga ttggggtagg tgaaaagaaa
     3001 attactcatc attggcatcg ctctggtggc agcagcggcg gcagccatca ccaccatcat
     3061 caccatcatt agtaatgact cgagcgataa ttcactcctc aggtgcaggc tgcctatcag
     3121 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     3181 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     3241 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     3301 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     3361 acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc atcagtatat
     3421 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat
     3481 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca
     3541 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct
     3601 tctcttatga agatccctcg acctgcagcc caagcttggc gtaatcatgg tcatagctgt
     3661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3781 tgcccgcttt ccagtcggga aacctgtcgt gccagcggat cgatccgctg cattaatgaa
     3841 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3901 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3961 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4021 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4081 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4141 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4201 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     4261 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4321 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4381 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4441 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4501 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4561 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4621 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4681 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctg
//
