LOCUS       c3960._C-vect_|_pCMV_-_ 5239 bp ds-DNA     circular     22-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    4..1254
                     /label="PKS5"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4..1254
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="ASRRVEAAFDGQAVELGATIPAANGNGTHQSIKVPGHRQVTPGKTTIMAIGRAVPANTTFNDGLADHYIQEFNLQDPVLQAKLRRLCETTTVKTRYLVVNKEILDEHPEFLVDGAATVSQRLAITGEAVTQLGHEAATAAIKEWGRPASEITHLVYVSSSEIRLPGGDLYLAQLLGLRSDVNRVMLYMLGCYGGASGIRVAKDLAENNPGSRVLLITSECTLIGYKSLSPDRPYDLVGAALFGDGAAAMIMGKDPIPVLERAFFELDWAGQSFIPGTNKTIDGRLSEEGISFKLGRELPKLIESNIQGFCDPILKRAGGLKYNDIFWAVHPGGPAILNAVQKQLDLAPEKLQTARQVLRDYGNISSSTCIYVLDYMRHQSLKLKEANDNVNTEPEWGLLLAFGPGVTIEGALLRNLC"
     rep_origin      complement(1685..1991)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2035..2139
                     /label="AmpR promotor"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        2182..2498
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        2302..2498
                     /label="SV40 early promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2536..3327
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      3859..4541
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        4698..5001
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        5002..5205
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
ORIGIN
        1 atgGCTAGTC GTCGTGTCGA GGCAGCATTC GATGGACAAG CAGTAGAACT GGGTGCTACT
       61 ATTCCTGCCG CCAATGGCAA CGGAACTCAT CAGTCAATTA AGGTTCCTGG ACATCGTCAA
      121 GTGACTCCAg gcAAGACTAC GATTATGGCT ATTGGCCGTG CTgtgCCCGC TAACACGACG
      181 TTCAATGATG GTTTGGCAGA CCATTACATC CAGGAGTTCA ATCTGcaaGA TCCAGTCCTT
      241 CAGGCTAAAC TGCGTCGTTT GTGCgaaACC ACAACCGTGA AAACGCGTTA TCTGGTCGTC
      301 AATAAGGAGA TTCTGGATGA GCATCCTgag TTCCTGGTTG ATGGAGCTGC AACCGTATCT
      361 CAGCGTCTGG CTATTACAGG CGAAGCTGTT ACCcaacttG GACATGAGGC AGCAACGGCT
      421 GCCATCAAAG AGTGGGGCCG TCCAGCCTCC GAAATTACCC ATCTTGTTTA TGTTTCCTCA
      481 TCCGAAATCC GTCTGCCAGG TGGCGACTTG TACCTGGCAC AATTGCTTGG CTTGCGTTCA
      541 GACGTGAACC GTGTAATGTT GTACATGCTG GGTTGCTATG GTGGTGCATC TGGAATCCGT
      601 GTTGCCAAAG ACTTGGCTGA GAATAACCCA GGCTCACGTG TACTTCTGAT CACGTCAGAA
      661 TGCACTTTGA TTGGATATAA GTCACTTTCC CCTGACCGTC CTTACGACCT GGTTGGAGCA
      721 GCTCTGTTCG GTGACGGTGC TGCCgctATG ATTATGGGAA AAGATCCAAT CCCTGTTTTG
      781 GAGCGTGCTT TCTTTGAGCT TGACTGGGCC GGTCAGTCTT TTATTCCTGG AACTAACAAA
      841 ACCATCGATG GTCGTTTGTC TGAAGAAGGC ATTTCCTTCA AGCTGGGTCG TGAACTGCCT
      901 AAGTTGATTG AATCCAATAT TCAGGGTTTC TGCGATCCTA TCTTGAAACG TGCTGGTGGT
      961 CTGAAGTATA ACGATATTTT CTGGGCAGTT CACCCAGGCG GACCAGCTAT CTTGAATGCT
     1021 GTCCAGAAAC AACTGGATTT GGCACCCGAG AAACTTCAGA CCGCACGTCA GGTTCTGCGT
     1081 GACTACGGTA ACATTTCTAG TTCCACCTGC ATTTACGTAC TGGACTATAT GCGTCATCAA
     1141 TCCTTGAAAC TGAAGGAAGC AAATGACAAC GTAAATACGG AACCAGAGTG GGGTCTGCTG
     1201 CTTGCATTTg gcCCCGGCGT CACTATCGAG GGTGCTTTGC TGCGTAACCT GTGCtgaAAG
     1261 CTTCGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT AGATAACTGA
     1321 TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC
     1381 TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG
     1441 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT
     1501 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC GTAAATTGTA
     1561 AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC
     1621 CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG
     1681 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA
     1741 GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT
     1801 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT
     1861 AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA
     1921 GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC
     1981 GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA
     2041 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA
     2101 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA AAGAACCAGC
     2161 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA
     2221 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG
     2281 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA
     2341 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC
     2401 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT
     2461 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA
     2521 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
     2581 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
     2641 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
     2701 CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
     2761 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
     2821 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
     2881 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
     2941 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
     3001 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
     3061 AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
     3121 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
     3181 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
     3241 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
     3301 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG
     3361 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
     3421 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
     3481 TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG ACAATACCGG
     3541 AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT GTTGGGTCGT
     3601 TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC
     3661 CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG
     3721 GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCCTCAGGTT
     3781 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
     3841 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
     3901 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
     3961 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
     4021 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
     4081 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
     4141 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
     4201 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
     4261 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
     4321 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
     4381 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
     4441 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
     4501 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
     4561 TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC
     4621 GTATTACCGC CATGCATTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
     4681 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
     4741 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
     4801 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
     4861 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
     4921 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
     4981 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
     5041 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
     5101 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
     5161 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT
     5221 CAGATCCGCT AGCgccacc
//