LOCUS       Exported                5309 bp ds-DNA     circular SYN 05-APR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 5, 2024 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    5..1268
                     /label=EF1-a
     CDS             1270..2947
                     /codon_start=1
                     /translation="MTTQDVIVNDQNDQKQCSNDVIFRSRLPDIYIPNHLPLHDYIFEN
                     ISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLT
                     FLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTD
                     SDAIPENCLRFSELTQSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVT
                     SVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLL
                     EQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFPN
                     AKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKP
                     GEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKG
                     FQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQ
                     VVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMNSGS"
     CDS             1270..2940
                     /label=At4CL2
     misc_feature    2957..3078
                     /label=bgh terminator
     misc_feature    3083..5309
                     /label=DVL1
ORIGIN
        1 ggagaagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
       61 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      121 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      181 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      241 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      301 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      361 acgggttatg gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg
      421 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
      481 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
      541 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
      601 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
      661 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
      721 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
      781 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
      841 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
      901 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
      961 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     1021 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     1081 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     1141 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     1201 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     1261 tgtcgtgaaa tgaccacgca agatgtaata gtaaatgatc aaaatgatca aaagcaatgt
     1321 tccaatgacg ttatcttccg atcacgttta cccgatatat atattcccaa tcatttgccc
     1381 ctgcacgatt acatttttga gaatatatcc gagtttgccg ccaaaccttg cctcatcaac
     1441 gggccgacag gagaagtcta tacgtacgca gacgtccatg tcacaagccg aaaactggct
     1501 gctgggctcc ataacttagg cgttaagcaa cacgacgtag tcatgatttt gcttccaaac
     1561 tctccggaag ttgtacttac tttcttggcc gcttccttca tcggcgccat tacaacctcc
     1621 gccaatccct tcttcactcc ggcagaaatt tccaaacagg ctaaggcttc cgcagctaaa
     1681 ttgatcgtaa cacagtcacg atatgttgac aaaattaaga acctccagaa tgatggcgtc
     1741 ttgatagtaa ctactgattc tgacgcaatt ccagagaact gtttgaggtt ttcagagttg
     1801 acacaaagtg aagaaccccg tgtcgactct attccagaga aaatttcccc cgaggatgtc
     1861 gttgctctgc cgttttcatc tggtaccaca gggctgccaa agggtgtaat gctcacgcac
     1921 aaaggacttg tgacttctgt cgctcaacag gtcgatgggg aaaatccaaa cctctacttc
     1981 aaccgtgatg atgtgattct ctgcgtcttg ccaatgttcc acatttacgc cctcaactca
     2041 ataatgcttt gctccttgag agtgggagcc acaatactca ttatgcctaa gtttgagatt
     2101 acacttctcc tcgaacagat acaacgatgt aaggtaactg tcgctatggt ggtgccaccg
     2161 atagtgttgg ccatagcaaa gtccccggag acagagaaat atgacttatc ttcagtgaga
     2221 atggtaaagt ctggagcagc acctcttggt aaagaattag aggatgccat atccgccaaa
     2281 tttcctaatg ccaagttagg tcaggggtac ggcatgacgg aagctggtcc ggttttggca
     2341 atgtctctcg gctttgctaa ggagcctttt cccgtgaagt ccggcgcatg tggaacggtt
     2401 gtgcgaaatg cagagatgaa gattctcgat ccggacacag gcgactcatt accgcgtaac
     2461 aagccaggag agatatgtat aagagggaac cagatcatga agggttatct gaatgatccg
     2521 ctggctactg cttctactat agacaaagat ggttggctcc atacggggga tgtcggcttt
     2581 atcgatgatg acgatgaatt atttatcgtc gataggctca aagagctcat caagtacaaa
     2641 ggatttcaag tcgcacctgc cgaattggaa tccttgttga taggtcaccc tgaaataaac
     2701 gacgtagctg tggtagccat gaaagaagaa gatgctgggg aggttcccgt tgcctttgtt
     2761 gtcaggtcca aggacagcaa cattagtgag gacgaaatca aacaatttgt atccaaacag
     2821 gtcgtattct ataaacgtat caataaagta ttctttacgg actctattcc aaaagcaccg
     2881 agcggcaaga tattaagaaa agacctgcga gccaggttgg caaacgggtt gatgaactca
     2941 ggtagttaac tagcttagtt aactagctgt gccttctagt tgccagccat ctgttgtttg
     3001 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3061 aaatgaggaa attgcatccg ctatgtcttc tactagtagc ggccgctgca gtccggcaaa
     3121 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
     3181 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     3241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3361 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     4081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgagtc ccgtcaagtc
     4141 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg
     4201 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     4261 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     4321 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     4381 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     4441 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     4501 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     4561 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     4621 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     4681 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4741 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4801 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4861 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4921 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctgga gcaagacgtt
     4981 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     5041 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     5101 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagct cgagtgccac
     5161 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     5221 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     5281 ggccgcttct agagactagt ggaagacat
//
