LOCUS       Exported                6803 bp ds-DNA     circular SYN 05-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 5, 2024 from SnapGene Viewer 4.2.11
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(540..644)
                     /label=AmpR promotor
     rep_origin      complement(737..1354)
                     /direction=LEFT
                     /label=oriV
     misc_feature    1384..1531
                     /label=TDNA-LB
     promoter        1664..2009
                     /label=CaMV 35S promoter
     CDS             2100..3285
                     /codon_start=1
                     /translation="MAPSVEEIRKAQRAQGPATVLAIGTATPPNCVYQKDYPDYYFRVT
                     NSEHMTELKEKFRRMCDKSMIEKRYMLLTEDLLKENPSMCAYMGSSLDARQDLVVAEVP
                     RLGKEAAQKAIKEWGQPKSKITHVIMCTTSGVDMPGADYQLTKLLGLRPSVKRFMMYQQ
                     GCFAGGTVLRLAKDVAENNKGARVLVVCSEITAICFRGPTDTHLDSMVGQALFGDGSGA
                     VIIGADPDLTVERPIFELVWTAQTILPDSEGAIDGHLREVGLTLHLLKDVPGLISKNIT
                     KALVEAFNPLGISDWNELFWVAHPGGPAILDQVEEKLALKPEKMKATRQVLNDYGNMSS
                     ACVLFILDEMRKKSLENGHSTTGEGLEWGVLFGFGPGLTVETVVLHSVPVVNSGS"
     misc_feature    2100..3275
                     /label=PzPKS2
     terminator      3303..4010
                     /label=OCS terminator
     misc_feature    complement(4045..4172)
                     /label=RB short
                     /label=RB\short
     misc_feature    complement(4196..5677)
                     /label=RK2 trfa (no Esp3I)
                     /label=RK2\trfa\(no\Esp3I)
     misc_feature    5688..6477
                     /label=pUC ori
                     /label=pUC\ori
     CDS             complement(join(6485..6803,1..539))
                     /label=AP r
                     /label=AP\r
ORIGIN
        1 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
       61 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      121 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      181 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      241 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
      301 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      361 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      421 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      481 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      541 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      601 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg
      661 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
      721 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      781 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      841 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      901 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      961 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
     1021 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
     1081 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
     1141 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
     1201 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
     1261 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
     1321 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcgcaaagga gatcctgatc
     1381 tgactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     1441 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     1501 ataacacatt gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtga
     1561 agacaagcaa gaattcaagc ttggaggtca acatggtgga gcacgacact ctggtctact
     1621 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
     1681 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     1741 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     1801 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1861 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
     1921 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1981 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
     2041 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
     2101 tggccccatc tgtggaagaa atcaggaagg cccagagagc tcagggcccg gccactgtcc
     2161 tcgccatcgg caccgccacc ccacccaact gcgtctatca gaaggactat ccggactact
     2221 acttccgtgt cactaactca gagcacatga ccgaattgaa ggagaagttc aggagaatgt
     2281 gcgacaaatc aatgatcgag aagcgttaca tgcttttgac cgaggatctt ctcaaggaga
     2341 accccagcat gtgcgcttac atgggatcct cccttgacgc aagacaggac ttggtcgtcg
     2401 ctgaggtccc aaggctcggc aaagaggccg cacagaaggc catcaaggag tggggccaac
     2461 ccaagtctaa gatcacccac gtcatcatgt gcaccacttc aggtgtcgac atgcctggcg
     2521 cagactacca gctcactaag cttctcggcc ttcgtccttc cgtcaagcga ttcatgatgt
     2581 accagcaagg ttgcttcgct ggtggaaccg ttcttcgact cgctaaggac gtggctgaga
     2641 acaacaaggg agcacgagtc ctcgtcgttt gctcagagat caccgccatc tgtttccgtg
     2701 gcccaacgga cacccacctg gactccatgg tgggacaagc cttattcgga gatggttcag
     2761 gagccgtcat catcggagcc gatccagact taaccgtgga gaggccaatc ttcgagctgg
     2821 tctggaccgc ccagaccatc ctcccagact ccgagggagc catcgacggt caccttagag
     2881 aagtcgggtt gactctccat ctcctcaagg acgttccagg gcttatctca aagaacatca
     2941 ccaaggcact cgtggaggcc tttaacccac ttggaatctc tgactggaac gagcttttct
     3001 gggtcgcaca ccccggtgga cccgctatcc ttgaccaggt tgaggagaaa ctcgctctca
     3061 agcctgagaa gatgaaggct acaaggcaag tgcttaatga ctacgggaac atgtcaagcg
     3121 cttgtgtgct gtttatctta gatgagatga ggaagaagtc tttggagaac ggtcattcta
     3181 ctacaggtga aggattggaa tggggtgttc tctttgggtt tggacctggg cttacggttg
     3241 agactgtcgt gctgcacagt gtccctgttg taaactcagg tagttaacta gcttaatagg
     3301 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
     3361 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     3421 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     3481 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
     3541 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
     3601 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
     3661 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
     3721 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
     3781 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
     3841 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
     3901 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
     3961 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaccgctact
     4021 attgtcttct gcacgaagtg gtttaaacta tcagtgtttg acaggatata ttggcgggta
     4081 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     4141 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttccccaga tcaggcgctg
     4201 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
     4261 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
     4321 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
     4381 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
     4441 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
     4501 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
     4561 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
     4621 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
     4681 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
     4741 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
     4801 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
     4861 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
     4921 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
     4981 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
     5041 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
     5101 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc aagacgacgc
     5161 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
     5221 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
     5281 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
     5341 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
     5401 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
     5461 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
     5521 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
     5581 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
     5641 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggatctgcc aggaaccgta
     5701 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5761 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5821 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5881 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5941 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6001 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6061 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6121 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6181 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6241 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6301 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6361 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6421 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6481 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6541 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6601 ccagtgctgc aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa
     6661 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6721 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6781 acgttgttgc cattgctaca ggc
//
