LOCUS       pGAPZ-PzPKS             4520 bp ds-DNA     circular     25-JUL-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     MCS             932..945
                     /label="MCSplus"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    947..2122
                     /label="PzPKS2"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             947..2122
                     /label="Translation 947-2122"
                     /translation="MAPSVEEIRKAQRAQGPATVLAIGTATPPNCVYQKDYPDYYFRVTNSEHMTELKEKFRRMCDKSMIEKRYMLLTEDLLKENPSMCAYMGSSLDARQDLVVAEVPRLGKEAAQKAIKEWGQPKSKITHVIMCTTSGVDMPGADYQLTKLLGLRPSVKRFMMYQQGCFAGGTVLRLAKDVAENNKGARVLVVCSEITAICFRGPTDTHLDSMVGQALFGDGSGAVIIGADPDLTVERPIFELVWTAQTILPDSEGAIDGHLREVGLTLHLLKDVPGLISKNITKALVEAFNPLGISDWNELFWVAHPGGPAILDQVEEKLALKPEKMKATRQVLNDYGNMSSACVLFILDEMRKKSLENGHSTTGEGLEWGVLFGFGPGLTVETVVLHSVPVVN"
     CDS             2178..2195
                     /label="6xHis"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     terminator      2199..2540
                     /label="AOX1 transcription terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2541..2952
                     /label="TEF1 promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        2953..3020
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             3021..3395
                     /label="Zeo(R)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     terminator      3396..3713
                     /label="CYC1 transcription terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      complement(3724..4397)
                     /label="pUC origin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4453..928
                     /label="GAP prom"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 acctaaacgc ttaatgcctc agacgtttta atgcctctca acacctccaa ggttgctttc
       61 ttgagcatgc ctactaggaa ctttaacgaa ctgtggggtt gcagacagtt tcaggcgtgt
      121 cccgaccaat atggcctact agactctctg aaaaatcaca gttttccagt agttccgatc
      181 aaattaccat cgaaatggtc ccataaacgg acatttgaca tccgttcctg aattatagtc
      241 ttccaccgtg gatcatggtg ttcctttttt tcccaaagaa tatcagcatc ccttaactac
      301 gttaggtcag tgatgacaat ggaccaaatt gttgcaaggt ttttcttttt ctttcatcgg
      361 cacatttcag cctcacatgc gactattatc gatcaatgaa atccatcaag attgaaatct
      421 taaaattgcc cctttcactt gacaggatcc ttttttgtag aaatgtcttg gtgtcctcgt
      481 ccaatcaggt agccatctct gaaatatctg gctccgttgc aactccgaac gacctgctgg
      541 caacgtaaaa ttctccgggg taaaacttaa atgtggagta atggaaccag aaacgtctct
      601 tcccttctct ctccttccac cgcccgttac cgtccctagg aaattttact ctgctggaga
      661 gcttcttcta cggccccctt gcagcaatgc tcttcccagc attacgttgc gggtaaaacg
      721 gaggtcgtgt acccgaccta gcagcccagg gatggaaaag tcccggccgt cgctggcaat
      781 aatagcgggc ggacgcatgt catgagatta ttggaaacca ccagaatcga atataaaagg
      841 cgaacacctt tcccaatttt ggtttctcct gacccaaaga ctttaaattt aatttatttg
      901 tccctatttc aatcaattga acaactatca attcgaaGAG GAATTcATGG CCCCATCTGT
      961 GGAAGAAATC AGGAAGGCCC AGAGAGCTCA GGGCCCGGCC ACTGTCCTCG CCATCGGCAC
     1021 CGCCACCCCA CCCAACTGCG TCTATCAGAA GGACTATCCG GACTACTACT TCCGTGTCAC
     1081 TAACTCAGAG CACATGACCG AATTGAAGGA GAAGTTCAGG AGAATGTGCG ACAAATCAAT
     1141 GATCGAGAAG CGTTACATGC TTTTGACCGA GGATCTTCTC AAGGAGAACC CCAGCATGTG
     1201 CGCTTACATG GGATCCTCCC TTGACGCAAG ACAGGACTTG GTCGTCGCTG AGGTCCCAAG
     1261 GCTCGGCAAA GAGGCCGCAC AGAAGGCCAT CAAGGAGTGG GGCCAACCCA AGTCTAAGAT
     1321 CACCCACGTC ATCATGTGCA CCACTTCAGG TGTCGACATG CCTGGCGCAG ACTACCAGCT
     1381 CACTAAGCTT CTCGGCCTTC GTCCTTCCGT CAAGCGATTC ATGATGTACC AGCAAGGTTG
     1441 CTTCGCTGGT GGAACCGTTC TTCGACTCGC TAAGGACGTG GCTGAGAACA ACAAGGGAGC
     1501 ACGAGTCCTC GTCGTTTGCT CAGAGATCAC CGCCATCTGT TTCCGTGGCC CAACGGACAC
     1561 CCACCTGGAC TCCATGGTGG GACAAGCCTT ATTCGGAGAT GGTTCAGGAG CCGTCATCAT
     1621 CGGAGCCGAT CCAGACTTAA CCGTGGAGAG GCCAATCTTC GAGCTGGTCT GGACCGCCCA
     1681 GACCATCCTC CCAGACTCCG AGGGAGCCAT CGACGGTCAC CTTAGAGAAG TCGGGTTGAC
     1741 TCTCCATCTC CTCAAGGACG TTCCAGGGCT TATCTCAAAG AACATCACCA AGGCACTCGT
     1801 GGAGGCCTTT AACCCACTTG GAATCTCTGA CTGGAACGAG CTTTTCTGGG TCGCACACCC
     1861 CGGTGGACCC GCTATCCTTG ACCAGGTTGA GGAGAAACTC GCTCTCAAGC CTGAGAAGAT
     1921 GAAGGCTACA AGGCAAGTGC TTAATGACTA CGGGAACATG TCAAGCGCTT GTGTGCTGTT
     1981 TATCTTAGAT GAGATGAGGA AGAAGTCTTT GGAGAACGGT CATTCTACTA CAGGTGAAGG
     2041 ATTGGAATGG GGTGTTCTCT TTGGGTTTGG ACCTGGGCTT ACGGTTGAGA CTGTCGTGCT
     2101 GCACAGTGTC CCTGTTGTAA ACtcataATc tagaacaaaa actcatctca gaagaggatc
     2161 tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttgtagcctt agacatgact
     2221 gttcctcagt tcaagttggg cacttacgag aagaccggtc ttgctagatt ctaatcaaga
     2281 ggatgtcaga atgccatttg cctgagagat gcaggcttca tttttgatac ttttttattt
     2341 gtaacctata tagtatagga ttttttttgt cattttgttt cttctcgtac gagcttgctc
     2401 ctgatcagcc tatctcgcag ctgatgaata tcttgtggta ggggtttggg aaaatcattc
     2461 gagtttgatg tttttcttgg tatttcccac tcctcttcag agtacagaag attaagtgag
     2521 accttcgttt gtgcggatcc cccacacacc atagcttcaa aatgtttcta ctcctttttt
     2581 actcttccag attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac
     2641 agcatactaa attttccctc tttcttcctc tagggtgtcg ttaattaccc gtactaaagg
     2701 tttggaaaag aaaaaagaga ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa
     2761 atttttatca cgtttctttt tcttgaaatt ttttttttta gtttttttct ctttcagtga
     2821 cctccattga tatttaagtt aataaacggt cttcaatttc tcaagtttca gtttcatttt
     2881 tcttgttcta ttacaacttt ttttacttct tcttcattag aaagaaagca tagcaatcta
     2941 atctaagggg cggtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     3001 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     3061 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     3121 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     3181 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     3241 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     3301 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     3361 actgcgtgca cttcgtggcc gaggagcagg actgacacgt ccgacggcgg cccacgggtc
     3421 ccaggcctcg gagatccgtc ccccttttcc tttgtcgata tcatgtaatt agttatgtca
     3481 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     3541 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     3601 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     3661 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa gctggagacc
     3721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     3841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3961 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     4261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4441 gtcatgagat cagatctctg ctactctggt cccaagtgaa ccaccttttg gaccctattg
     4501 accggacctt aacttgccaa
//