LOCUS       c2539._LM_|_P4f:_HygR_c 8858 bp ds-DNA     circular     09-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      144..761
                     /label="oriV"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             913..1920
                     /label="spec\orf?"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2098..2887)
                     /label="pUC\ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     rep_origin      complement(2852..2852)
                     /label="ORI"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2898..4379
                     /label="RK2\TrfA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(4413..4563)
                     /label="LB"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    4588..6882
                     /label="HygR cassette"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        4680..4859
                     /label="NOS promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4895..4950
                     /label="TMV Omega"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     terminator      6175..6882
                     /label="OCS terminator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(6910..7394)
                     /label="act2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(7450..7485)
                     /label="Linker"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(7490..8286)
                     /label="nnLuz_v4"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(8291..8802)
                     /label="35S(0.4kb)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(8855..124)
                     /label="RB"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
       61 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
      121 gccagccgcc tttgcgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt
      181 ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg
      241 ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag gggcggacgt
      301 tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc aggctcgatt
      361 tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct gattttacgc
      421 gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt attgacactt
      481 gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg ggcagagtgc
      541 tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca gaaaatccag
      601 catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt
      661 taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg
      721 cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac gcgggcctcc
      781 catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa atggcagcgc
      841 tggccaattc ccgaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
      901 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
      961 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     1021 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1081 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     1141 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1201 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1261 ccacacagcg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1321 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1381 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     1441 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     1501 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     1561 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     1621 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     1681 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     1741 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     1801 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     1861 gatcagttgg aagaatttgt ccattacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     1921 taatgtctag ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag
     1981 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     2041 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgctgtcag
     2101 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2161 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2401 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2581 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2641 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2701 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2761 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2821 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2881 tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt
     2941 cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg
     3001 gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac
     3061 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg
     3121 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg
     3181 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
     3241 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
     3301 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag
     3361 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
     3421 tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
     3481 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa
     3541 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
     3601 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
     3661 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
     3721 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
     3781 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
     3841 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
     3901 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
     3961 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
     4021 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
     4081 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
     4141 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
     4201 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
     4261 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt
     4321 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagcccctgc
     4381 tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc gagtggtgat
     4441 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     4501 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
     4561 ggttgaacac tctgtgggtc tcattacgaa ttcccatggg gagagcggag aattaaggga
     4621 gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag
     4681 aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac
     4741 atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa
     4801 atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag
     4861 agtctcatat tcactctcat actgtatttt tacaacaatt accaacaaca acaaacaaca
     4921 aacaacatta caattactat ttacaattac aatgAAAAAG CCTGAACTCA CCGCGACGTC
     4981 TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGtGTCTCC GACCTGATGC AGCTCTCGGA
     5041 GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT
     5101 AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
     5161 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC TGACCTATTG
     5221 CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG CCTGAAACCG AACTGCCCGC
     5281 TGTTCTGCAG GTAAATTTCT AGTTTTTCTC CTTCATTTTC TTGGTTAGGA CCCTTTTCTC
     5341 TTTTTATTTT TTTGAGCTTT GATCTTTCTT TAAACTGATC TATTTTTTAA TTGATTGGTT
     5401 ATGGTGTAAA TATTACATAG CTTTAACTGA TAATCTGATT ACTTTATTTC GTGTGTCTAT
     5461 GATGATGATG ATAACTGCAG CCGGTCGCGG AGGCCATGGA TGCGATCGCT GCGGCCGATC
     5521 TTAGCCAGAC GAGCGGGTTC GGCCCATTCG GACCGCAAGG AATCGGTCAA TACACTACAT
     5581 GGCGTGATTT CATATGCGCG ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG
     5641 ACGACACCGT CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG
     5701 ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT GTCCTGACGG
     5761 ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC GATGTTCGGG GATTCCCAAT
     5821 ACGAGGTCGC CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC
     5881 GCTACTTCGA GCGGAGGCAT CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC
     5941 TCCGCATTGG TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG
     6001 CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT GTCGGGCGTA
     6061 CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG CTGTGTAGAA GTACTCGCCG
     6121 ATAGTGGAAA CCGACGCCCC AGCACTCGTC CGAGGGCAAA GGAATAGGCT TGTCCTGCTT
     6181 TAATGAGATA TGCGAGAAGC CTATGATCGC ATGATATTTG CTTTCAATTC TGTTGTGCAC
     6241 GTTGTAAAAA ACCTGAGCAT GTGTAGCTCA GATCCTTACC GCCGGTTTCG GTTCATTCTA
     6301 ATGAATATAT CACCCGTTAC TATCGTATTT TTATGAATAA TATTCTCCGT TCAATTTACT
     6361 GATTGTACCC TACTACTTAT ATGTACAATA TTAAAATGAA AACAATATAT TGTGCTGAAT
     6421 AGGTTTATAG CGACATCTAT GATAGAGCGC CACAATAACA AACAATTGCG TTTTATTATT
     6481 ACAAATCCAA TTTTAAAAAA AGCGGCAGAA CCGGTCAAAC CTAAAAGACT GATTACATAA
     6541 ATCTTATTCA AATTTCAAAA GTGCCCCAGG GGCTAGTATC TACGACACAC CGAGCGGCGA
     6601 ACTAATAACG CTCACTGAAG GGAACTCCGG TTCCCCGCCG GCGCGCATGG GTGAGATTCC
     6661 TTGAAGTTGA GTATTGGCCG TCCGCTCTAC CGAAAGTTAC GGGCACCATT CAACCCGGTC
     6721 CAGCACGGCG GCCGGGTAAC CGACTTGCTG CCCCGAGAAT TATGCAGCAT TTTTTTGGTG
     6781 TATGTGGGCC CCAAATGAAG TGCAGGTCAA ACCTTGACAG TGACGACAAA TCGTTGGGCG
     6841 GGTCCAGGGC GAATTTTGCG ACAACATGTC GAGGCTCAGC AGGACCGCTc agagaattcg
     6901 catgcagcga aatggtgcga tcttaaaata tctgactttg tgctagttta agttcatttg
     6961 tgaatggaac acatgtaacg ctttaactat tcagaatatc agttccaagt gaagatggag
     7021 gtagcaaaag taagactaat aaccaaatca ataaaaatct tatacaatac ttatattaac
     7081 attgcaaaga gtttcaaggt ttttcttctg agttttgaaa gaaattatag ggaacaaaag
     7141 gaataaagag gcatcaattc gatcactcag agctacaaaa caatgggact aaaacgcaaa
     7201 acgaaagcgg tttttttttt tgaaacgcag acgtaagtaa aaacccagag agtttgtcac
     7261 acacaagtgc atcatagaaa cgaaaacaaa aagggaaatg aaacaaacaa atggagaagc
     7321 aaatataaaa gaaaaaaaga aactttgatc ccattcataa aaccccagct ttttaagcct
     7381 ttgatcttga gagcaagcTC ACAGATGGAT GACGAAGTGG GACAGGCTAG AGAACCAGTT
     7441 CTTGCATGCA GAATTCGAAG CTTGAGCTCG AGATCTGAGT CCGGAcgaaT TGGCATTTTC
     7501 GACAATCTTA CCAGCAGGAT CGTTAGTCAT GTAGCCAATA CTAGCTTCGA CTATCATCTC
     7561 GACAACTCTG GCCTCCTCTT CGTTCCTTGG TGCGTAAAGG AAGGTCCACT CAGTAGGAGG
     7621 GCCTGGGACT CCTGGTCCGG CCAGTGGGTG ACGCTGGCCC CACCCCTTCT TCAAAACTTC
     7681 CTTACCATCC TGaggAGCAA GAGCCAAGTG CAGagaGCAA TCATGGTAAT CATGCATGTG
     7741 AGCGATCTCA CGCTTGGTTC TTCTTACCGT ATCGTGTGAG GGAATGTTAA GAGGATCATT
     7801 AATAGGGATA GAGGGGAGAA GGAAGATGGC CTTTGCATGA AACTCAAATT TTGACGTACT
     7861 GAGAAAAGTT TTGTCTGTGT GTCTTGCTTG CACACGTGGG ATCACCTTAT CGATAAGTTC
     7921 TCTGGTTGCA AACTCTTGAT CTACCAGCTG AGTACGCTGA CGCTGTATAA TGTGGGAGGT
     7981 TATGGATGGC CTtggCCCTT CACGAAACGG CAAGTCGGCA AGCCAACGCC TCTTTTCTGG
     8041 AAGCCTGTCA TAGATTGCCA ACCCTTTCTG CTCTTGCCTA AAAAGTGACA AGACACAAAC
     8101 agtGATATAG CCTCGGAAGT TCTGTGGGGC ATAGCTCGGA CCGACTTCAA GGAACGTTTG
     8161 ATAGTCTCTT CGGATAATTG GGAATGCTAT TGCACTTGCC AAGACTACGC CGCAGGTAAT
     8221 TGCGATGGAT CGGGATGACA ATTTGCTCAG TCTCTCaagG AGACTAGACA AAGAGATagt
     8281 agaCCTcatt gtatcgataa ttgtaaatgt aattgtaatg ttgtttgttg tttgttgttg
     8341 ttggtaattg ttgtaaaaat gagctcttat actcgagcgt gtcctctcca aatgaaatga
     8401 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     8461 gtcagtggag atgtcacatc aatccacttg ctttgtagac gtggttggaa cctcttcttt
     8521 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagagagatc
     8581 ttgaatgata gcctttcctt tatcgcaatg atggcatttg taggagccac cttccttttc
     8641 tactgtcctt tcgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     8701 aattatcctt tgttgaaaag tctcaatagc cctttgatct tctgagactg tatctttgac
     8761 atttttggag tagaccagag tgtcgtgctc caccatgttg acctccTGTG tgagaccgag
     8821 gatgcacatg tgaccgaGGG Acacgaagtg atccgttt
//