LOCUS       C17_pAAV_CMV-CD4        6271 bp DNA     circular SYN 11-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Vector CMS238 digested with XbaI and BamHI
            f1: same as 490R
            r1:CATTGTgaaAAGTATGGCTCCGGCcaaCTTGCGCCGCGCATTAAAgctgccgctg
            f2:GttgGCCGGAGCCATACTTttcACAATGTTGGCTACCAGAAATTTTTCGGAGCTGGCCG
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
FEATURES             Location/Qualifiers
     source          1..6271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /locus_tag="ITR"
                     /label=ITR
     misc_feature    151..708
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     misc_feature    753..770
                     /locus_tag="T7 promoter primer"
                     /label=T7 promoter primer
     misc_feature    779..784
                     /locus_tag="HindIII"
                     /label=HindIII
     misc_feature    785..790
                     /locus_tag="Kozak Sequence"
                     /label=Kozak Sequence
     misc_feature    791..850
                     /locus_tag="Ig k-chain signal sequence"
                     /label=Ig k-chain signal sequence
     misc_feature    791..793
                     /locus_tag="START Codon"
                     /label=START Codon
     misc_feature    869..874
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    875..901
                     /locus_tag="HA tag 1"
                     /label=HA tag 1
     misc_feature    902..928
                     /locus_tag="HA tag 2"
                     /label=HA tag 2
     misc_feature    929..1606
                     /locus_tag="human CD4"
                     /label=human CD4
     misc_feature    1607..1636
                     /label=10 aa linker
     misc_feature    1637..1675
                     /locus_tag="Linker"
                     /label=13 aa Linker
                     /label=Linker
     CDS             1676..2416
                     /locus_tag="sC Turbo G74"
                     /label=sC Turbo G74
     misc_feature    1715..1717
                     /locus_tag="I87V"
                     /label=I87V
     misc_feature    1799..1801
                     /locus_tag="G115G"
                     /label=G115G
     misc_feature    1808..1810
                     /locus_tag="R118S"
                     /label=R118S
     misc_feature    1874..1876
                     /locus_tag="E141K"
                     /label=E141K
     misc_difference 1877..1879
                     /locus_tag="Q142R"
                     /label=Q142R
     misc_feature    1901..1903
                     /locus_tag="S150G"
                     /label=S150G
     misc_feature    1904..1906
                     /locus_tag="L151P"
                     /label=L151P
     misc_feature    1931..1933
                     /locus_tag="V160A"
                     /label=V160A
     misc_feature    2027..2029
                     /locus_tag="T192A"
                     /label=T192A
     misc_difference 2033..2035
                     /locus_tag="K194I"
                     /label=K194I
     misc_feature    2078..2080
                     /locus_tag="M209V"
                     /label=M209V
     misc_feature    2174..2176
                     /locus_tag="M241T"
                     /label=M241T
     misc_feature    2240..2242
                     /locus_tag="S263P"
                     /label=S263P
     misc_structure  2324..2326
                     /locus_tag="G291G"
                     /label=G291G
     misc_feature    2366..2368
                     /locus_tag="I305V"
                     /label=I305V
     misc_feature    2417..2437
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    2438..2452
                     /locus_tag="LINKER"
                     /label=LINKER
     misc_feature    2453..2509
                     /locus_tag="M13 from GCaMP6s"
                     /label=M13 from GCaMP6s
     misc_feature    2510..2539
                     /locus_tag="misc_feature"
                     /label=misc_feature
     misc_feature    2520..2539
                     /locus_tag="Part of Primer"
                     /label=Part of Primer
     misc_feature    2540..3256
                     /locus_tag="eGFP"
                     /label=eGFP
     misc_feature    complement(3257..3262)
                     /locus_tag="double stop"
                     /label=double stop
     misc_feature    3263..3268
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    3275..3506
                     /locus_tag="PolyA"
                     /label=PolyA
     misc_feature    3535..3675
                     /locus_tag="ITR(1)"
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atggagacag acacactcct gctatgggta ctgctgctct gggttccagg
      841 ttccactggt gacggggccc agccggccag atcttaccca tacgatgtgc cagattacgc
      901 ctatccctat gacgtccctg attacgctct cgacttccag aaggcctcca gcatagtcta
      961 taagaaagag ggggaacagg tggagttctc cttcccactc gcctttacag ttgaaaagct
     1021 gacgggcagt ggcgagctgt ggtggcaggc ggagagggct tcctcctcca agtcttggat
     1081 cacctttgac ctgaagaaca aggaagtgtc tgtaaaacgg gttacccagg accctaagct
     1141 ccagatgggc aagaagctcc cgctccacct caccctgccc caggccttgc ctcagtatgc
     1201 tggctctgga aacctcaccc tggcccttga agcgaaaaca ggaaagttgc atcaggaagt
     1261 gaacctggtg gtgatgagag ccactcagct ccagaaaaat ttgacctgtg aggtgtgggg
     1321 acccacctcc cctaagctga tgctgagctt gaaactggag aacaaggagg caaaggtctc
     1381 gaagcgggag aaggcggtgt gggtgctgaa ccctgaggcg gggatgtggc agtgtctgct
     1441 gagtgactcg ggacaggtcc tgctggaatc caacatcaag gttctgccca catggtccac
     1501 cccggtgcag ccaatggccc tgattgtgct ggggggcgtc gccggcctcc tgcttttcat
     1561 tgggctaggc atcttcttct gtgtcaggtg ccggcaccga aggcgcaagg gctcgggctc
     1621 gacctcgggc tcgggcagcg gtggctctag aggttctggt ggcagctctg gaggtggaca
     1681 gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     1741 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     1801 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     1861 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     1921 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     1981 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     2041 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     2101 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     2161 tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     2221 aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca atagacccgt
     2281 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     2341 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     2401 gcggtctgcc gaaaagggct cgggcgggag taccggtggt agtgggagcg gtcgtcgtaa
     2461 gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcag gtggaagtgg
     2521 atcaggcagc ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2581 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2641 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2701 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2761 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2821 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2881 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2941 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3001 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3061 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3121 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3181 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3241 ggacgagctg tacaagtaat aagaattcct cgagctagag ctcgctgatc agcctcgact
     3301 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     3361 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3421 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3481 gaagacaata gcaggcatgc tggggaggta accacgtgcg gaccgagcgg ccgcaggaac
     3541 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     3601 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     3661 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattc
     3721 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     3781 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     3841 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     3901 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     3961 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4021 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4081 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4141 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     4201 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     4261 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4321 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4381 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4441 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4501 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4561 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4621 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4681 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4741 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4801 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4861 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4921 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4981 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5041 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5101 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5161 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5221 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5281 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat
     5341 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5401 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5461 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5521 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5581 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5641 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5701 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5761 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5821 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5881 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5941 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6001 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6061 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6121 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6181 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6241 ctggcctttt gctggccttt tgctcacatg t
//
