LOCUS       C25_pAAV_CMV-CD4        7630 bp DNA     circular SYN 11-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Vector CMS238 digested with XbaI and BamHI
            f1: same as 490R
            r1:CATTGTgaaAAGTATGGCTCCGGCcaaCTTGCGCCGCGCATTAAAgctgccgctg
            f2:GttgGCCGGAGCCATACTTttcACAATGTTGGCTACCAGAAATTTTTCGGAGCTGGCCG
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
FEATURES             Location/Qualifiers
     source          1..7630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /locus_tag="ITR"
                     /label=ITR
     misc_feature    151..708
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     misc_feature    753..770
                     /locus_tag="T7 promoter primer"
                     /label=T7 promoter primer
     misc_feature    779..784
                     /locus_tag="HindIII"
                     /label=HindIII
     misc_feature    785..790
                     /locus_tag="Kozak Sequence"
                     /label=Kozak Sequence
     misc_feature    791..793
                     /locus_tag="START Codon"
                     /label=START Codon
     misc_feature    794..853
                     /locus_tag="Ig k-chain signal sequence"
                     /label=Ig k-chain signal sequence
     misc_feature    869..874
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    875..901
                     /locus_tag="HA tag 1"
                     /label=HA tag 1
     misc_feature    902..928
                     /locus_tag="HA tag 2"
                     /label=HA tag 2
     misc_feature    929..1606
                     /locus_tag="human CD4"
                     /label=human CD4
     misc_feature    1607..1675
                     /locus_tag="Linker"
                     /label=Linker
     CDS             1676..2416
                     /locus_tag="sC Turbo G74"
                     /label=sC Turbo G74
     misc_feature    1715..1717
                     /locus_tag="I87V"
                     /label=I87V
     misc_feature    1799..1801
                     /locus_tag="G115G"
                     /label=G115G
     misc_feature    1808..1810
                     /locus_tag="R118S"
                     /label=R118S
     misc_feature    1874..1876
                     /locus_tag="E141K"
                     /label=E141K
     misc_difference 1877..1879
                     /locus_tag="Q142R"
                     /label=Q142R
     misc_feature    1901..1903
                     /locus_tag="S150G"
                     /label=S150G
     misc_feature    1904..1906
                     /locus_tag="L151P"
                     /label=L151P
     misc_feature    1931..1933
                     /locus_tag="V160A"
                     /label=V160A
     misc_feature    2027..2029
                     /locus_tag="T192A"
                     /label=T192A
     misc_difference 2033..2035
                     /locus_tag="K194I"
                     /label=K194I
     misc_feature    2078..2080
                     /locus_tag="M209V"
                     /label=M209V
     misc_feature    2174..2176
                     /locus_tag="M241T"
                     /label=M241T
     misc_feature    2240..2242
                     /locus_tag="S263P"
                     /label=S263P
     misc_structure  2324..2326
                     /locus_tag="G291G"
                     /label=G291G
     misc_feature    2366..2368
                     /locus_tag="I305V"
                     /label=I305V
     misc_feature    2417..2437
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    2438..2452
                     /locus_tag="LINKER"
                     /label=LINKER
     misc_feature    2453..2509
                     /locus_tag="M13 from GCaMP6s"
                     /label=M13 from GCaMP6s
     misc_feature    2510..2539
                     /locus_tag="misc_feature"
                     /label=misc_feature
     misc_feature    2520..2539
                     /locus_tag="Part of Primer"
                     /label=Part of Primer
     misc_feature    2540..3256
                     /locus_tag="eGFP"
                     /label=eGFP
     misc_feature    3245..3264
                     /label=Overlap
     misc_feature    3257..3259
                     /label=stop
     misc_feature    3272..3824
                     /label=IRES
     misc_feature    3833..3852
                     /label=Overlap
     misc_feature    3833..3838
                     /label=Kozak sequence
                     /label=Kozak
     misc_feature    3839..3841
                     /label=START codon
                     /label=Ig k-chain signal sequence
     misc_feature    3842..4282
                     /locus_tag="Calmodulin from GCaMP6s"
                     /label=Calmodulin from GCaMP6s
     misc_feature    4283..4312
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    4352..4393
                     /locus_tag="V5 tag"
                     /label=V5 tag
     misc_feature    4394..4399
                     /locus_tag="NheI"
                     /label=NheI
     CDS             4400..4615
                     /locus_tag="sN Turbo L73"
                     /label=sN Turbo L73
     misc_feature    4589..4591
                     /locus_tag="Q65P"
                     /label=Q65P
     misc_feature    complement(4616..4621)
                     /locus_tag="double stop"
                     /label=double stop
     misc_feature    4622..4627
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    4634..4865
                     /locus_tag="PolyA"
                     /label=PolyA
     misc_feature    4894..5034
                     /locus_tag="ITR(1)"
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca tagtaatcaa ttacggggtc attagttcat
      181 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      241 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      301 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
      361 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      421 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      481 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      541 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      601 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      661 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
      721 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
      781 gcttgccacc atggagacag acacactcct gctatgggta ctgctgctct gggttccagg
      841 ttccactggt gacggggccc agccggccag atcttaccca tacgatgtgc cagattacgc
      901 ctatccctat gacgtccctg attacgctct cgacttccag aaggcctcca gcatagtcta
      961 taagaaagag ggggaacagg tggagttctc cttcccactc gcctttacag ttgaaaagct
     1021 gacgggcagt ggcgagctgt ggtggcaggc ggagagggct tcctcctcca agtcttggat
     1081 cacctttgac ctgaagaaca aggaagtgtc tgtaaaacgg gttacccagg accctaagct
     1141 ccagatgggc aagaagctcc cgctccacct caccctgccc caggccttgc ctcagtatgc
     1201 tggctctgga aacctcaccc tggcccttga agcgaaaaca ggaaagttgc atcaggaagt
     1261 gaacctggtg gtgatgagag ccactcagct ccagaaaaat ttgacctgtg aggtgtgggg
     1321 acccacctcc cctaagctga tgctgagctt gaaactggag aacaaggagg caaaggtctc
     1381 gaagcgggag aaggcggtgt gggtgctgaa ccctgaggcg gggatgtggc agtgtctgct
     1441 gagtgactcg ggacaggtcc tgctggaatc caacatcaag gttctgccca catggtccac
     1501 cccggtgcag ccaatggccc tgattgtgct ggggggcgtc gccggcctcc tgcttttcat
     1561 tgggctaggc atcttcttct gtgtcaggtg ccggcaccga aggcgcaagg gctcgggctc
     1621 gacctcgggc tcgggcagcg gtggctctag aggttctggt ggcagctctg gaggtggaca
     1681 gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     1741 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     1801 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     1861 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     1921 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     1981 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     2041 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     2101 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     2161 tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     2221 aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca atagacccgt
     2281 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     2341 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     2401 gcggtctgcc gaaaagggct cgggcgggag taccggtggt agtgggagcg gtcgtcgtaa
     2461 gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcag gtggaagtgg
     2521 atcaggcagc ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     2581 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     2641 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     2701 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     2761 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2821 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2881 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2941 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     3001 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     3061 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     3121 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     3181 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     3241 ggacgagctg tacaagtagc ccccccccct aacgttactg gccgaagccg cttggaataa
     3301 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3361 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3421 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3481 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3541 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3601 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3661 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3721 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3781 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cagccaccat
     3841 ggaccaactg actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa
     3901 ggacggggat gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca
     3961 gaaccccaca gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg
     4021 cacaatcgac ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac
     4081 ggaagaagaa attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag
     4141 tgcagcagag cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt
     4201 tgatgaaatg atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt
     4261 tgtacaaatg atgacagcga agaagggctc gggctcgacc tcgggctcgg gcagcggtgg
     4321 ctctggaggt tctggtggca gctctggagg tggcaagccc atccccaacc ccctgctggg
     4381 cctggacagc accgctagca aagacaatac tgtgcctctg aagctgatcg ctctcctggc
     4441 taatggcgag ttccatagtg gcgaacagct gggagaaacc ctgggcatgt ccagggccgc
     4501 tatcaacaag cacattcaga ctctgcgcga ctggggcgtg gacgtgttca ccgtgcccgg
     4561 aaagggctac tctctgcccg agcctatccc gctgctgaac gctaaacaga ttctgtaata
     4621 agaattcctc gagctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     4681 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4741 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4801 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4861 ggggaggtaa ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     4921 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4981 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     5041 tgatgcggta ttttctcctt acgcatctgt gcggtattca caccgcatac gtcaaagcaa
     5101 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     5341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5401 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
     5461 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5521 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     5581 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5641 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5701 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5761 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6181 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6661 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7621 gctcacatgt
//
