LOCUS       C28_Syn-CaM-V5-s        4440 bp DNA     circular SYN 11-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Template 155A digested with BamHI, NheI
            f1:gaagcttGCCACCatgCACCTGAGAATCCACGCGAGACGG
            f2:gtcgtgcctgagagcgcagtcgagaagaagcttGCCACCatgCACCTGAGAATC
            r1:GTATGGCTCCGGCcAGCTTGCGCCGCGC
            f2:GCGCGGCGCAAGCTgGCCGGAGCCATAC
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
FEATURES             Location/Qualifiers
     source          1..4440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /locus_tag="ITR"
                     /label=ITR
     misc_feature    151..628
                     /locus_tag="synapsin promoter"
                     /label=synapsin promoter
     misc_feature    637..642
                     /locus_tag="HindIII"
                     /label=HindIII
     misc_feature    643..648
                     /locus_tag="Kozak Sequence"
                     /label=Kozak Sequence
     misc_feature    649..651
                     /locus_tag="START codon"
                     /label=START codon
     misc_feature    652..1092
                     /locus_tag="Calmodulin from GCaMP6s"
                     /label=Calmodulin from GCaMP6s
     misc_feature    1093..1122
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    1162..1203
                     /locus_tag="V5 tag"
                     /label=V5 tag
     misc_feature    1204..1209
                     /locus_tag="NheI"
                     /label=NheI
     CDS             1210..1425
                     /locus_tag="sN Turbo L73"
                     /label=sN Turbo L73
     misc_feature    1399..1401
                     /locus_tag="Q65P"
                     /label=Q65P
     misc_feature    complement(1426..1431)
                     /locus_tag="double stop"
                     /label=double stop
     misc_feature    1432..1437
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    1444..1675
                     /locus_tag="PolyA"
                     /label=PolyA
     misc_feature    1704..1844
                     /locus_tag="ITR(1)"
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttgccaccat ggaccaactg
      661 actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa ggacggggat
      721 gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca gaaccccaca
      781 gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg cacaatcgac
      841 ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac ggaagaagaa
      901 attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag tgcagcagag
      961 cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt tgatgaaatg
     1021 atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt tgtacaaatg
     1081 atgacagcga agaagggctc gggctcgacc tcgggctcgg gcagcggtgg ctctggaggt
     1141 tctggtggca gctctggagg tggcaagccc atccccaacc ccctgctggg cctggacagc
     1201 accgctagca aagacaatac tgtgcctctg aagctgatcg ctctcctggc taatggcgag
     1261 ttccatagtg gcgaacagct gggagaaacc ctgggcatgt ccagggccgc tatcaacaag
     1321 cacattcaga ctctgcgcga ctggggcgtg gacgtgttca ccgtgcccgg aaagggctac
     1381 tctctgcccg agcctatccc gctgctgaac gctaaacaga ttctgtaata agaattcctc
     1441 gagctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1501 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1561 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1621 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggaggtaa
     1681 ccacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     1741 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     1801 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     1861 ttttctcctt acgcatctgt gcggtattca caccgcatac gtcaaagcaa ccatagtacg
     1921 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     1981 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     2041 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     2101 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     2161 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     2221 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     2281 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     2341 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     2401 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     2461 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     2521 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     2581 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     2641 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     2701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     2761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     2821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     2881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     2941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     3001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     3061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     3121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     3181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     3241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     3301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     3361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     3421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc
     3481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     3541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     3601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     3661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     3721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3901 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
