LOCUS       C27_Syn-CD4-sTC-        6129 bp DNA     circular SYN 11-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 11, 2024 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Template 155A digested with BamHI, NheI
            f1:gaagcttGCCACCatgCACCTGAGAATCCACGCGAGACGG
            f2:gtcgtgcctgagagcgcagtcgagaagaagcttGCCACCatgCACCTGAGAATC
            r1:GTATGGCTCCGGCcAGCTTGCGCCGCGC
            f2:GCGCGGCGCAAGCTgGCCGGAGCCATAC
            r2:CTTATCGTCGTCATCCTTGTAGTCggatc
FEATURES             Location/Qualifiers
     source          1..6129
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /locus_tag="ITR"
                     /label=ITR
     misc_feature    151..628
                     /locus_tag="synapsin promoter"
                     /label=synapsin promoter
     misc_feature    637..642
                     /locus_tag="HindIII"
                     /label=HindIII
     misc_feature    643..648
                     /locus_tag="Kozak Sequence"
                     /label=Kozak Sequence
     misc_feature    649..651
                     /locus_tag="START Codon"
                     /label=START Codon
     misc_feature    652..711
                     /locus_tag="Ig k-chain signal sequence"
                     /label=Ig k-chain signal sequence
     misc_feature    727..732
                     /locus_tag="BglII"
                     /label=BglII
     misc_feature    733..759
                     /locus_tag="HA tag 1"
                     /label=HA tag 1
     misc_feature    760..786
                     /locus_tag="HA tag 2"
                     /label=HA tag 2
     misc_feature    787..1464
                     /locus_tag="human CD4"
                     /label=human CD4
     misc_feature    1465..1533
                     /locus_tag="Linker"
                     /label=Linker
     CDS             1534..2274
                     /locus_tag="sC Turbo G74"
                     /label=sC Turbo G74
     misc_feature    1573..1575
                     /locus_tag="I87V"
                     /label=I87V
     misc_feature    1657..1659
                     /locus_tag="G115G"
                     /label=G115G
     misc_feature    1666..1668
                     /locus_tag="R118S"
                     /label=R118S
     misc_feature    1732..1734
                     /locus_tag="E141K"
                     /label=E141K
     misc_difference 1735..1737
                     /locus_tag="Q142R"
                     /label=Q142R
     misc_feature    1759..1761
                     /locus_tag="S150G"
                     /label=S150G
     misc_feature    1762..1764
                     /locus_tag="L151P"
                     /label=L151P
     misc_feature    1789..1791
                     /locus_tag="V160A"
                     /label=V160A
     misc_feature    1885..1887
                     /locus_tag="T192A"
                     /label=T192A
     misc_difference 1891..1893
                     /locus_tag="K194I"
                     /label=K194I
     misc_feature    1936..1938
                     /locus_tag="M209V"
                     /label=M209V
     misc_feature    2032..2034
                     /locus_tag="M241T"
                     /label=M241T
     misc_feature    2098..2100
                     /locus_tag="S263P"
                     /label=S263P
     misc_structure  2182..2184
                     /locus_tag="G291G"
                     /label=G291G
     misc_feature    2224..2226
                     /locus_tag="I305V"
                     /label=I305V
     misc_feature    2275..2295
                     /locus_tag="Linker"
                     /label=Linker
     misc_feature    2296..2310
                     /locus_tag="LINKER"
                     /label=LINKER
     misc_feature    2311..2367
                     /locus_tag="M13 from GCaMP6s"
                     /label=M13 from GCaMP6s
     misc_feature    2368..2397
                     /locus_tag="misc_feature"
                     /label=misc_feature
     misc_feature    2378..2397
                     /locus_tag="Part of Primer"
                     /label=Part of Primer
     misc_feature    2398..3114
                     /locus_tag="eGFP"
                     /label=eGFP
     misc_feature    complement(3115..3120)
                     /locus_tag="double stop"
                     /label=double stop
     misc_feature    3121..3126
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    3133..3364
                     /locus_tag="PolyA"
                     /label=PolyA
     misc_feature    3393..3533
                     /locus_tag="ITR(1)"
                     /label=ITR
                     /label=ITR(1)
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaagaagc ttgccaccat ggagacagac
      661 acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga cggggcccag
      721 ccggccagat cttacccata cgatgtgcca gattacgcct atccctatga cgtccctgat
      781 tacgctctcg acttccagaa ggcctccagc atagtctata agaaagaggg ggaacaggtg
      841 gagttctcct tcccactcgc ctttacagtt gaaaagctga cgggcagtgg cgagctgtgg
      901 tggcaggcgg agagggcttc ctcctccaag tcttggatca cctttgacct gaagaacaag
      961 gaagtgtctg taaaacgggt tacccaggac cctaagctcc agatgggcaa gaagctcccg
     1021 ctccacctca ccctgcccca ggccttgcct cagtatgctg gctctggaaa cctcaccctg
     1081 gcccttgaag cgaaaacagg aaagttgcat caggaagtga acctggtggt gatgagagcc
     1141 actcagctcc agaaaaattt gacctgtgag gtgtggggac ccacctcccc taagctgatg
     1201 ctgagcttga aactggagaa caaggaggca aaggtctcga agcgggagaa ggcggtgtgg
     1261 gtgctgaacc ctgaggcggg gatgtggcag tgtctgctga gtgactcggg acaggtcctg
     1321 ctggaatcca acatcaaggt tctgcccaca tggtccaccc cggtgcagcc aatggccctg
     1381 attgtgctgg ggggcgtcgc cggcctcctg cttttcattg ggctaggcat cttcttctgt
     1441 gtcaggtgcc ggcaccgaag gcgcaagggc tcgggctcga cctcgggctc gggcagcggt
     1501 ggctctagag gttctggtgg cagctctgga ggtggacagc tggacggcgg gagcgtggca
     1561 gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag
     1621 agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa
     1681 tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga
     1741 ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag
     1801 ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag
     1861 ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt
     1921 ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg
     1981 atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc tacgctgatc
     2041 cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca
     2101 cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa
     2161 gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac
     2221 ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga aaagggctcg
     2281 ggcgggagta ccggtggtag tgggagcggt cgtcgtaagt ggaataagac aggtcacgca
     2341 gtcagagcta taggtcggct gagctcaggt ggaagtggat caggcagcgg tggatctatg
     2401 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2461 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2521 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2581 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2641 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2701 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2761 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2821 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2881 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2941 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3001 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3061 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa
     3121 gaattcctcg agctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     3181 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     3241 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     3301 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     3361 gggaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
     3421 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     3481 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     3541 gatgcggtat tttctcctta cgcatctgtg cggtattcac accgcatacg tcaaagcaac
     3601 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3661 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3781 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     3841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3901 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg
     3961 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4021 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     4081 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     4141 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     4201 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     4261 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4321 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4381 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4441 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4501 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4561 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4621 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4681 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4741 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4801 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4861 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4921 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4981 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5041 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5101 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5161 gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5221 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5281 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5341 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5401 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5461 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5521 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5581 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     5641 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5701 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5761 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5821 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5881 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5941 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6001 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6061 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6121 ctcacatgt
//
