LOCUS       Exported                8409 bp ds-DNA     linear   UNA 07-FEB-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    849..1499
                     /locus_tag="ymStaygold_Piatkevich†"
                     /label=ymStaygold_Piatkevich†
     misc_feature    1506..1580
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1581..2291
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4664..5463
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5895..6755
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7838..7867
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc cccgggacta
      841 gtaagcttat ggcttctact ccattcaagt tccaattgaa gggtactatt aacggtaagt
      901 ctttcactgt tgaaggtgaa ggtgaaggta actctcatga aggttctcat aagggtaagt
      961 atgtttgtac ttctggtaag ttgccaatgt cttgggctgc tttgggtacc tctttcggtt
     1021 acggtatgaa gtactacact aagtatccat ctggtttaaa gaactggttc catgaagtta
     1081 tgccagaagg tttcacttac gatagacata ttcaatacaa gggtgatggt tctattcatg
     1141 ctaagcatca acatttcatg aagaacggta cttaccataa cattgttgaa ttcactggtc
     1201 aagattttaa ggaaaactct ccagttttga ctggtgatat gaatgtttct ttgccaaacg
     1261 atgttcaaca tattccaaga gatgatggtg ttgaatgtcc agttactttg ttgtacccat
     1321 tgttgtctga taagtctaag tgtgttgaag ctcatcaaaa cactatttgt aagccattgc
     1381 ataaccaacc agctccagat gttccatatc attggattag aaagcaatac actcaatcta
     1441 aggatgatac tgaagaaaga gatcatattt gtcaatctga aactttggaa gctcatttgt
     1501 ccggagaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg
     1561 gggatccacc ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca
     1621 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     1681 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1741 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     1801 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     1861 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     1921 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     1981 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     2041 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     2101 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     2161 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     2221 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     2281 tgtacaagta gcggccgcac ccagctttct tgtacaaagt ggtgtagtta attcatgatg
     2341 gccgctgcag gtcgacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
     2401 attacgcgcg gatccagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     2461 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     2521 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     2581 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     2641 aaaacganaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     2701 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccaattc actggccgtc
     2761 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2821 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2881 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     2941 tgcggtattt cacaccgcat aatcggatcg tacttgttac ccatcattga attttgaaca
     3001 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
     3061 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
     3121 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
     3181 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
     3241 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     3301 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     3361 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     3421 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     3481 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     3541 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     3601 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     3661 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     3721 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     3781 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     3841 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     3901 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     3961 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     4021 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     4081 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     4141 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     4201 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
     4261 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
     4321 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
     4381 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
     4441 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
     4501 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
     4561 aagctgtcaa acatgagaat tgggtaataa ctgatataat taaattgaag ctctaatttg
     4621 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     4681 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     4741 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     4801 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     4861 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     4921 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     4981 agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     5041 tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     5101 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     5161 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     5221 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     5281 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     5341 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     5401 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     5461 catgatttat cttcgtttcc tgcatgtttt tgttctgtgc agttgggtta agaatactgg
     5521 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     5581 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaggaaaccg
     5641 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa agctaattct tgaagacgaa
     5701 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5761 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5821 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5881 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5941 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6001 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6061 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6121 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6181 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6241 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6301 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6361 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6421 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6481 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6541 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6601 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6661 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6721 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6781 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6841 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6901 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6961 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7021 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7081 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7141 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7201 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7261 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7321 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7381 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7441 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7501 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7561 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7621 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7681 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7741 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7801 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7861 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7921 attacgccaa gcttaccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     7981 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     8041 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     8101 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     8161 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     8221 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     8281 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8341 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8401 tccattcgc
//
