LOCUS       Exported                8418 bp ds-DNA     linear   UNA 07-FEB-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1508
                     /locus_tag="ymStayGold_Ando"
                     /label=ymStayGold_Ando
     misc_feature    1515..1589
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1590..2300
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4673..5472
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5904..6764
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7847..7876
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtttcta
      841 ctggtgaaga attgtttact ggtgttgttc cattcaagtt ccaattaaag ggtactatta
      901 acggtaagtc tttcactgtt gaaggtgaag gtgaaggtaa ctctcatgaa ggttctcata
      961 agggtaagta cgtttgtact tctggtaagt tgccaatgtc ttgggctgct ttaggtactt
     1021 ctttcggtta cggtatgaag tactacacta agtacccatc tggtttgaag aactggttcc
     1081 atgaagttat gcctgaaggt ttcacttacg atagacatat tcaatacaag ggtgatggtt
     1141 ctattcatgc taagcatcaa catttcatga agaacggtac ttaccataac attgttgaat
     1201 tcactggtca agatttcaag gaaaactccc cagttttgac tggtgatatg gatgtttctt
     1261 tgccaaacga agttcaacat attccaattg atgatggtgt tgaatgtact gttactttgc
     1321 aatacccatt gttgtctgat gaatctaagt gtgttgaagc ctaccaaaat actattatta
     1381 agccattgca taaccaacca gctccagatg ttccattcca ctggattaga aaacaataca
     1441 ctcaatctaa ggatgatact gaagaaagag atcatattat tcaatctgaa actttggaag
     1501 ctcatttgtc cggagagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc
     1561 ccggccctgg ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     1621 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1681 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1741 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1801 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1861 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1921 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1981 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2041 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2101 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2161 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2221 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2281 tggacgagct gtacaagtag cggccgcacc cagctttctt gtacaaagtg gtgtagttaa
     2341 ttcatgatgg ccgctgcagg tcgacctcga gggggggccc ggtacccaat tcgccctata
     2401 gtgagtcgta ttacgcgcgg atccagcttt ggacttcttc gccagaggtt tggtcaagtc
     2461 tccaatcaag gttgtcggct tgtctacctt gccagaaatt tacgaaaaga tggaaaaggg
     2521 tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat
     2581 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
     2641 ttaggtttta aaacganaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
     2701 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgccaattca
     2761 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     2821 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     2881 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     2941 acgcatctgt gcggtatttc acaccgcata atcggatcgt acttgttacc catcattgaa
     3001 ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg aacctgtata
     3061 ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc ctggagaaac
     3121 tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt
     3181 ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc attttgtaga
     3241 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
     3301 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
     3361 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     3421 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
     3481 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
     3541 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
     3601 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
     3661 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
     3721 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
     3781 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
     3841 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
     3901 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
     3961 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
     4021 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
     4081 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
     4141 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagctagag
     4201 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     4261 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     4321 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     4381 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     4441 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     4501 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcga
     4561 tccgatgata agctgtcaaa catgagaatt gggtaataac tgatataatt aaattgaagc
     4621 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     4681 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     4741 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     4801 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     4861 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     4921 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     4981 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     5041 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     5101 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     5161 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     5221 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     5281 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     5341 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     5401 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     5461 agctttcgac atgatttatc ttcgtttcct gcatgttttt gttctgtgca gttgggttaa
     5521 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     5581 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     5641 aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaaaa gctaattctt
     5701 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5761 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5821 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5881 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5941 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6001 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6061 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6121 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6181 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6241 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6301 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6361 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6421 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6481 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6541 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6601 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6661 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6721 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6781 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6841 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6901 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6961 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7021 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7081 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7141 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7201 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7261 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7321 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7381 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7441 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7501 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7561 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7621 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7681 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7741 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7801 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7861 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7921 atgaccatga ttacgccaag cttaccgcat caggaaattg taagcgttaa tattttgtta
     7981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     8341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     8401 cagggcgcgt ccattcgc
//
