LOCUS       Exported                8464 bp ds-DNA     linear   UNA 20-NOV-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    857..1543
                     /locus_tag="mscarleti3"
                     /label=mscarleti3
     primer_bind     complement(987..1005)
                     /locus_tag="mCherry-R"
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    1544..1624
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1625..2341
                     /locus_tag="mTurquoise2"
                     /label=mTurquoise2
                     /note="mTq2"
     misc_feature    4719..5518
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5950..6810
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7893..7922
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc tatcccggga
      841 tccaccggtc gccaccatgg atagcaccga ggcagtgatc aaggagttca tgcggttcaa
      901 ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      961 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1021 cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct tcatcaagca
     1081 ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca agtgggagcg
     1141 cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct ccctggagga
     1201 cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg acggccccgt
     1261 aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc ccgaggacgt
     1321 cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc
     1381 ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg ccttcaacat
     1441 cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga
     1501 acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctccggag agggcagagg
     1561 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctggggatc caccggtcgc
     1621 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1681 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1741 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1801 caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc ccgaccacat
     1861 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1921 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1981 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2041 gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg acaagcagaa
     2101 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     2161 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2221 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     2281 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2341 gtaaagcggc cgcacccagc tttcttgtac aaagtggtgt agttaattca tgatggccgc
     2401 tgcaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
     2461 gcgcggatcc agctttggac ttcttcgcca gaggtttggt caagtctcca atcaaggttg
     2521 tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa atcgttggta
     2581 gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt tttattatta
     2641 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     2701 ganaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     2761 atgaggtcgc tcttattgac cacacctcta ccggcatgcc aattcactgg ccgtcgtttt
     2821 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2881 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2941 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3001 tatttcacac cgcataatcg gatcgtactt gttacccatc attgaatttt gaacatccga
     3061 acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa tatatagtct
     3121 agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt gcatctattg
     3181 cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat cttgcacttc
     3241 aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg
     3301 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     3361 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg
     3421 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     3481 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa
     3541 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct
     3601 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta
     3661 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc
     3721 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga
     3781 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat
     3841 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg
     3901 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta
     3961 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt
     4021 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga
     4081 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt
     4141 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt
     4201 ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata ggaacttcgg
     4261 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
     4321 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
     4381 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
     4441 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
     4501 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
     4561 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcgatccg atgataagct
     4621 gtcaaacatg agaattgggt aataactgat ataattaaat tgaagctcta atttgtgagt
     4681 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca
     4741 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct
     4801 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca
     4861 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca
     4921 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga
     4981 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag
     5041 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta
     5101 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat
     5161 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg
     5221 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata
     5281 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg
     5341 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg
     5401 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct
     5461 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga
     5521 tttatcttcg tttcctgcat gtttttgttc tgtgcagttg ggttaagaat actgggcaat
     5581 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
     5641 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc
     5701 aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta attcttgaag acgaaagggc
     5761 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6181 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6661 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7561 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7621 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7681 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7741 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7801 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7861 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7921 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7981 gccaagctta ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8041 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8101 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     8161 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     8221 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     8281 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     8341 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     8401 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat
     8461 tcgc
//
