LOCUS       Exported                8426 bp ds-DNA     linear   UNA 30-AUG-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1505
                     /locus_tag="mVermilion"
                     /label=mVermilion
     regulatory      831..834
                     /regulatory_class="other"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /label=vertebrate consensus sequence for strong
                     initia...
                     /label=vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     primer_bind     complement(964..982)
                     /locus_tag="mCherry-R"
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1232..1251
                     /locus_tag="mCherry-F"
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1512..1586
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1587..2303
                     /locus_tag="mTurquoise2"
                     /label=mTurquoise2
                     /note="mTq2"
     misc_feature    4681..5480
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5912..6772
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7855..7884
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgtcta
      841 agggcgaaga gctgatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      901 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      961 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     1021 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgacttct
     1081 ttaagcagtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     1141 gcgtggtgac cgtgacccag gactcctccc tccaggacgg cgagttcatc tacaaggtga
     1201 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     1261 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1321 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1381 ccaagaagcc cgtgggcaac atcaagatgc ccggcgccta caacgtcaac atcaagttgg
     1441 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     1501 gcgcctccgg agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg
     1561 gccctgggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     1621 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1681 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1741 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gtcctggggc gtgcagtgct
     1801 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1861 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1921 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1981 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctactttagc gacaacgtct
     2041 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
     2101 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     2161 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     2221 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     2281 tcggcatgga cgagctgtac aagtaaagcg gccgcaccca gctttcttgt acaaagtggt
     2341 gtagttaatt catgatggcc gctgcaggtc gacctcgagg gggggcccgg tacccaattc
     2401 gccctatagt gagtcgtatt acgcgcggat ccagctttgg acttcttcgc cagaggtttg
     2461 gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg
     2521 gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta
     2581 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     2641 agtgactctt aggttttaaa acganaattc ttattcttga gtaactcttt cctgtaggtc
     2701 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     2761 ccaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2821 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2881 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     2941 ttctccttac gcatctgtgc ggtatttcac accgcataat cggatcgtac ttgttaccca
     3001 tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa
     3061 cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct
     3121 ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt
     3181 ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat
     3241 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     3301 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     3361 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     3421 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     3481 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     3541 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     3601 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     3661 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     3721 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     3781 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     3841 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     3901 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     3961 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     4021 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     4081 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     4141 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     4201 agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     4261 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     4321 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     4381 gcgtacttat atgcgtctat ttatgtagga tgaaaggtag tctagtacct cctgtgatat
     4441 tatcccattc catgcggggt atcgtatgct tccttcagca ctacccttta gctgttctat
     4501 atgctgccac tcctcaattg gattagtctc atccttcaat gctatcattt cctttgatat
     4561 tggatcgatc cgatgataag ctgtcaaaca tgagaattgg gtaataactg atataattaa
     4621 attgaagctc taatttgtga gtttagtata catgcattta cttataatac agttttttag
     4681 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
     4741 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
     4801 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
     4861 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
     4921 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
     4981 cccttagtat attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa
     5041 aggcctctag gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct
     5101 gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc
     5161 gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt
     5221 aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa
     5281 tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact
     5341 aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt
     5401 tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc
     5461 ttatatgtag ctttcgacat gatttatctt cgtttcctgc atgtttttgt tctgtgcagt
     5521 tgggttaaga atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc
     5581 aatctaagtc tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa
     5641 aaatttcaag gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaaaagc
     5701 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5761 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7621 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7681 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7741 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7801 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7861 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7921 aaacagctat gaccatgatt acgccaagct taccgcatca ggaaattgta agcgttaata
     7981 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     8041 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     8101 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     8161 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     8221 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     8281 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     8341 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     8401 cgccgctaca gggcgcgtcc attcgc
//
