LOCUS       Exported                7830 bp ds-DNA     linear   UNA 12-MAR-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     misc_feature    893..979
                     /locus_tag="Cyterm 29 aminoacids Rabbit cytochrome p450
                     sequenc"
                     /label=Cyterm 29 aminoacids Rabbit cytochrome
                     p450 seq...
                     /label=Cyterm 29 aminoacids Rabbit cytochrome p450
                     sequenc
     misc_feature    980..1007
                     /locus_tag="linker"
                     /label=linker
     misc_feature    1031..1708
                     /locus_tag="ymStayGold_Ando"
                     /label=ymStayGold_Ando
     misc_feature    4085..4884
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5316..6176
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7259..7288
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggctgctag cgctaccgga
      841 ctcagatctg gtaccccttg cagcttccag ttgtcaggaa gagaaggctc ccatggatcc
      901 tgtggtggtg ctggggctct gtctctcctg tttgcttctc ctttcactct ggaaacagag
      961 ctatggggga gggaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1021 ggtcgccacc atggtttcta ctggtgaaga attgtttact ggtgttgttc cattcaagtt
     1081 ccaattaaag ggtactatta acggtaagtc tttcactgtt gaaggtgaag gtgaaggtaa
     1141 ctctcatgaa ggttctcata agggtaagta cgtttgtact tctggtaagt tgccaatgtc
     1201 ttgggctgct ttaggtactt ctttcggtta cggtatgaag tactacacta agtacccatc
     1261 tggtttgaag aactggttcc atgaagttat gcctgaaggt ttcacttacg atagacatat
     1321 tcaatacaag ggtgatggtt ctattcatgc taagcatcaa catttcatga agaacggtac
     1381 ttaccataac attgttgaat tcactggtca agatttcaag gaaaactccc cagttttgac
     1441 tggtgatatg gatgtttctt tgccaaacga agttcaacat attccaattg atgatggtgt
     1501 tgaatgtact gttactttgc aatacccatt gttgtctgat gaatctaagt gtgttgaagc
     1561 ctaccaaaat actattatta agccattgca taaccaacca gctccagatg ttccattcca
     1621 ctggattaga aaacaataca ctcaatctaa ggatgatact gaagaaagag atcatattat
     1681 tcaatctgaa actttggaag ctcatttgta agcggccgca cccagctttc ttgtacaaag
     1741 tggtgtagtt aattcatgat ggccgctgca ggtcgacctc gagggggggc ccggtaccca
     1801 attcgcccta tagtgagtcg tattacgcgc ggatccagct ttggacttct tcgccagagg
     1861 tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa
     1921 gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt
     1981 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     2041 ttaaagtgac tcttaggttt taaaacgana attcttattc ttgagtaact ctttcctgta
     2101 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     2161 catgccaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2221 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2281 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2341 tattttctcc ttacgcatct gtgcggtatt tcacaccgca taatcggatc gtacttgtta
     2401 cccatcattg aattttgaac atccgaacct gggagttttc cctgaaacag atagtatatt
     2461 tgaacctgta taataatata tagtctagcg ctttacggaa gacaatgtat gtatttcggt
     2521 tcctggagaa actattgcat ctattgcata ggtaatcttg cacgtcgcat ccccggttca
     2581 ttttctgcgt ttccatcttg cacttcaata gcatatcttt gttaacgaag catctgtgct
     2641 tcattttgta gaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     2701 ctgcattttt acagaacaga aatgcaacgc gaaagcgcta ttttaccaac gaagaatctg
     2761 tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     2821 ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta ccaacaaaga
     2881 atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt ttctaacaaa
     2941 gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc ttgataactt
     3001 tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat tttctcttcc
     3061 ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc tgcgggtgca
     3121 ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt gcgcatactt
     3181 tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta tgaacggttt
     3241 cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt attgttttcg
     3301 attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat actagagata
     3361 aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa ggtggatggg
     3421 taggttatat agggatatag cacagagata tatagcaaag agatactttt gagcaatgtt
     3481 tgtggaagcg gtattcgcaa tattttagta gctcgttaca gtccggtgcg tttttggttt
     3541 tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag cgctctgaag ttcctatact
     3601 ttctagctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg
     3661 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat
     3721 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt
     3781 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg
     3841 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt
     3901 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg
     3961 atattggatc gatccgatga taagctgtca aacatgagaa ttgggtaata actgatataa
     4021 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     4081 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     4141 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     4201 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     4261 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     4321 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     4381 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     4441 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     4501 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     4561 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     4621 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     4681 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     4741 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     4801 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     4861 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcatgttt ttgttctgtg
     4921 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     4981 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     5041 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     5101 aagctaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5161 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7321 caggaaacag ctatgaccat gattacgcca agcttaccgc atcaggaaat tgtaagcgtt
     7381 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     7441 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     7501 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     7561 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     7621 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     7681 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     7741 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     7801 aatgcgccgc tacagggcgc gtccattcgc
//
