LOCUS       Exported                8442 bp ds-DNA     linear   UNA 12-APR-2024
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Apr 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1532
                     /locus_tag="ymBaojin"
                     /label=ymBaojin
     misc_feature    1539..1613
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1614..2324
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4697..5496
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5928..6788
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7871..7900
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtttcta
      841 agggtgaaga agaaaacatg gcttctactc cattcaagtt ccaattgaag ggtactatta
      901 acggtaagtc tttcactgtt gaaggtgaag gtgaaggtaa ctctcatgaa ggttctcaca
      961 agggtaagta cgtttgtact tctggtaagt tgccaatgtc ttgggctgct ttgggtacta
     1021 ctttcggtta cggtatgaag tactacacta agtacccatc tggtttaaag aactggttca
     1081 gagaagttat gccaggtggt ttcacttacg atagacatat tcaatacaag ggtgatggtt
     1141 ctattcatgc taagcatcaa catttcatga agaacggtac ttaccataac attgttgaat
     1201 tcactggtca agatttcaag gaaaattctc cagttttgac cggtgatatg aacgtttctt
     1261 tgccaaatga agttccacaa attccaagag atgatggtgt tgaatgtcca gttactttat
     1321 tgtacccatt gttgtctgat aagtctaagt atgttgaagc tcatcaatac actatttgta
     1381 agccattgca taaccaacca gctccagatg tcccatacca ttggattaga aagcaataca
     1441 ctcaatctaa ggatgatgct gaagaaagag atcatatttg tcaatctgaa actttagaag
     1501 ctcatttgaa gggtatggat gaattgtaca agtccggaga gggcagagga agtcttctaa
     1561 catgcggtga cgtggaggag aatcccggcc ctggggatcc accggtcgcc accatggtga
     1621 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1681 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1741 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1801 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     1861 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     1921 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     1981 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     2041 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     2101 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     2161 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     2221 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     2281 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagcggccg cacccagctt
     2341 tcttgtacaa agtggtgtag ttaattcatg atggccgctg caggtcgacc tcgagggggg
     2401 gcccggtacc caattcgccc tatagtgagt cgtattacgc gcggatccag ctttggactt
     2461 cttcgccaga ggtttggtca agtctccaat caaggttgtc ggcttgtcta ccttgccaga
     2521 aatttacgaa aagatggaaa agggtcaaat cgttggtaga tacgttgttg acacttctaa
     2581 ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag
     2641 tgtatacaaa ttttaaagtg actcttaggt tttaaaacga naattcttat tcttgagtaa
     2701 ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca
     2761 cacctctacc ggcatgccaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     2821 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     2881 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     2941 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataatcgga
     3001 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     3061 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     3121 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     3181 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     3241 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     3301 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     3361 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     3421 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     3481 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     3541 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     3601 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     3661 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     3721 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     3781 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     3841 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     3901 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     3961 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     4021 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     4081 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     4141 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     4201 agttcctata ctttctagct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     4261 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     4321 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     4381 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     4441 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     4501 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     4561 tcatttcctt tgatattgga tcgatccgat gataagctgt caaacatgag aattgggtaa
     4621 taactgatat aattaaattg aagctctaat ttgtgagttt agtatacatg catttactta
     4681 taatacagtt ttttagtttt gctggccgca tcttctcaaa tatgcttccc agcctgcttt
     4741 tctgtaacgt tcaccctcta ccttagcatc ccttcccttt gcaaatagtc ctcttccaac
     4801 aataataatg tcagatcctg tagagaccac atcatccacg gttctatact gttgacccaa
     4861 tgcgtctccc ttgtcatcta aacccacacc gggtgtcata atcaaccaat cgtaaccttc
     4921 atctcttcca cccatgtctc tttgagcaat aaagccgata acaaaatctt tgtcgctctt
     4981 cgcaatgtca acagtaccct tagtatattc tccagtagat agggagccct tgcatgacaa
     5041 ttctgctaac atcaaaaggc ctctaggttc ctttgttact tcttctgccg cctgcttcaa
     5101 accgctaaca atacctgggc ccaccacacc gtgtgcattc gtaatgtctg cccattctgc
     5161 tattctgtat acacccgcag agtactgcaa tttgactgta ttaccaatgt cagcaaattt
     5221 tctgtcttcg aagagtaaaa aattgtactt ggcggataat gcctttagcg gcttaactgt
     5281 gccctccatg gaaaaatcag tcaagatatc cacatgtgtt tttagtaaac aaattttggg
     5341 acctaatgct tcaactaact ccagtaattc cttggtggta cgaacatcca atgaagcaca
     5401 caagtttgtt tgcttttcgt gcatgatatt aaatagcttg gcagcaacag gactaggatg
     5461 agtagcagca cgttccttat atgtagcttt cgacatgatt tatcttcgtt tcctgcatgt
     5521 ttttgttctg tgcagttggg ttaagaatac tgggcaattt catgtttctt caacactaca
     5581 tatgcgtata tataccaatc taagtctgtg ctccttcctt cgttcttcct tctgttcgga
     5641 gattaccgaa tcaaaaaaat ttcaaggaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa
     5701 tgatgaattg aaaagctaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5761 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5821 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5881 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5941 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6001 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6061 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6121 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6181 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6241 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6301 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6361 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6421 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     6481 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     6541 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6601 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6661 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6721 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6781 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6841 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6901 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6961 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7021 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7081 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7141 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7201 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7261 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7321 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7381 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7441 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     7501 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     7561 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7621 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7681 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7861 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7921 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttacc gcatcaggaa
     7981 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     8041 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     8101 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8161 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8221 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8281 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8341 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8401 cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gc
//
