LOCUS       atg_2        2367 bp DNA     circular SYN 12-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   MIT
  TITLE     Direct Submission
  JOURNAL   Exported Apr 12, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     292..311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    512..549
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     misc_RNA        complement(583..658)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(679..927)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(737..756)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(907..927)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1034..1138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1139..1207,1208..1999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1357..1376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2170..2367,1..391)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2170..2367
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
       61 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
      121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
      181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
      241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
      301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
      361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      421 cctggccttt tgctggcctt ttgctcacat gtcatatgta gagaggtacc tcgagcggcc
      481 caagcttaaa aaaaattgcc tggaggaggc cggcttgtcg acgacggcgg tctccgtcgt
      541 caggatcatc cggggcggac gccggcattc gccgcgtggt tcgcaccgac tcggtgccac
      601 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaaccc
      661 atcctccagg caatacgcgg tgtttcgtcc tttccacaag atatataaag ccaagaaatc
      721 gaaatacttt caagttacgg taagcatatg atagtccatt ttaaaacata attttaaaac
      781 tgcaaactac ccaagaaatt attactttct acgtcacgta ttttgtacta atatctttgt
      841 gtttacagtc aaattaattc taattatctc tctaacagcc ttgtatcgta tatgcaaata
      901 tgaaggaatc atgggaaata ggccctcttc ctgcccgacc ttggtaccgg atccagtcga
      961 ctgaattggt tcctttaaag cctgcttttt tgtacagcta gcgacgtcag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggatctcg
     1861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2341 ccaccacttc aagaactctg tagcacc
//
