LOCUS       Tg57_(E9)_KO            7113 bp ds-DNA     circular     27-JUL-2023
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: Tg57 (E9) KO.gb
FEATURES             Location/Qualifiers
     misc_feature    complement(159..339)
                     /label="pr-tRNA(Gly)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(320..339)
                     /label="Copy of Empty 162HR gRNA2 - Hph Marker insertion REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: TGCGCCGACCCGGAATCGAACCGGGGGCCCATCGATGGCA"
     primer          340..359
                     /label="YALI1_D17249g_tg57 FWD"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: TGCCATCGATGGGCCCCCGGTTCGATTCCGGGTCGGCGCA"
     misc_feature    340..359
                     /label="GA Homology"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    360..379
                     /label="gRNA tg57"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    380..458
                     /label="tracrRNA gRNA scaffhold"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    459..478
                     /label="TerRPR1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    479..640
                     /label="HR up"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    641..660
                     /label="Insertion Site"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    658..819
                     /label="Downstream HR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    820..839
                     /label="GA Homology"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(820..839)
                     /label="YALI1_D17249g_tg57 REV"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: CTGAAGTGGGGATTTAAATGCGGCCGCGAATGCACGCGAT"
     primer          840..879
                     /label="Copy of Empty 162HR gRNA2 - Hph Marker insertion FWD"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTGCATTCGCGGCCGCATTTAAATCCCCACTTCAGAAGTTCCTATACTTTCTAGA"
     misc_feature    complement(1346..2137)
                     /label="amp"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2259..2847)
                     /label="Ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        3776..4777
                     /label="PrExp"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             4778..5806
                     /label="Hph"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: aph(4)-Ia note: confers resistance to hygromycin product: aminoglycoside phosphotransferase from E. coli transl_table: 1 translation: MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE*"
     terminator      5807..6039
                     /label="Tcyc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="label: Tcyc note: /product="
     misc_feature    complement(6049..7034)
                     /label="POT1"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(7035..140)
                     /label="CEN1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
ORIGIN
        1 TTTATGTTAA CTACTGTGAG GCATTAAATA GAGCTTGATA TATAAAATGT TACATTTCAC
       61 AGTCTGAACT TTTGCAGATT ACCTAATTTG GTAAGATATT AATTATGAAC TGAAAGTTGA
      121 TGGCATCCCT AAATTTGATG CAACGGAATG CGTGCGATAG TGAATCATTG CTAACAGATC
      181 AAGTAATCGT ATCAATCGTT CCACGTCTTA TCCAGACTCG ATTTCCGCCC TAACCTAAGG
      241 AAACTGCTGA GTAATTTCGA GGTTGGTTCC TAACAAAAGC CAAGAAACGC GCCGATGGTT
      301 TAGTGGTAAA ATCCATCGTT GCCATCGATG GGCCCCCGGT TCGATTCCGG GTCGGCGCAA
      361 GTTTTTGAAA CCCTGCTTGG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC
      421 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGGTGCTT TTTTTGTGTG GGTACGGTGA
      481 AAGAATCACG TGAATCCTTG AACACGTGTC ATTCCAATGG TACTGCTCTC CAGTTTCCAG
      541 CTCCAATATT GACACCAACT CGTCGACTAT TAAAGTTGTG TGGAGAGAGG TTGCATGAGT
      601 CATATCCAAT GGCCGAGGAA GATGGGATTC TGGGGTACAT GACTGAAGAG CTCTTCAATG
      661 TCGGTCAAAC GTGAATCTTC AGATAGTCCC GGGGTCCAGA CAAAGGACAA GCGGCTTGCA
      721 TGTGTCGAAT GTCGGCAGCA AAAGGTCAAA TGCGACGGCC AGGAGGGCTC CGACTGCTCT
      781 CGATGCAAGC GAAAGGGCAT TCCTTGCGTG GTGCAAAAGA TCGCGTGCAT TCGCGGCCGC
      841 ATTTAAATCC CCACTTCAGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCACTATAGA
      901 GTCGAATAAG GGCGACACCC CATAATTAGC CCGGGCGAAA GGCCCAGTCT TTCGACTGAG
      961 CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGT CCCCACACTA
     1021 CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG GACCACCGCG
     1081 CTACTGCCGC CAGGCAAACA AGGGGTGTTA TGAGCCATAT TCAGGTATAA ATGGGCTCGC
     1141 GATAATGTTC AGAATTGGTT AATTGGTTGT AACACTGACC CCTATTTGTT TATTTTTCTA
     1201 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     1261 TTGAAAAAGG AAGAATATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     1321 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     1381 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     1441 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     1501 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     1561 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     1621 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     1681 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     1741 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     1801 GCGTGACACC ACGATGCCTG TAGCGATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     1861 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     1921 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCCGGAGC
     1981 CGGTGAGCGT GGTTCTCGCG GTATCATCGC AGCGCTGGGG CCAGATGGTA AGCCCTCCCG
     2041 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     2101 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAAGCA GAGCATTACG CTGACTTGAC
     2161 GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG TTACGCGCGC GTCGTTCCAC
     2221 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC
     2281 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT
     2341 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT
     2401 ACTGTTCTTC TAGTGTAGCC GTAGTTAGCC CACCACTTCA AGAACTCTGT AGCACCGCCT
     2461 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT
     2521 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG
     2581 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA
     2641 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG
     2701 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG
     2761 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC
     2821 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
     2881 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
     2941 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
     3001 AGCGAGTCAG TGAGCGAGGA AGCGGAAGGC GAGAGTAGGG AACTGCCAGG CATCAAACTA
     3061 AGCAGAAGGC CCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTC TGTGTTGTAA
     3121 AACGACGGCC AGTCTTAAGC TCGGGCCCCC TGGGCGGTTC TGATAACGAG TAATCGTTAA
     3181 TCCGCAAATA ACGTAAAAAC CCGCTTCGGC GGGTTTTTTT ATGGGGGGAG TTTAGGGAAA
     3241 GAGCATTTGT CAGAATATTT AAGGGCGCCT GTCACTTTGC TTGATATATG AGAATTATTT
     3301 AACCTTATAA ATGAGAAAAA AGCAACGCAC TTTAAATAAG ATACGTTGCT TTTTCGATTG
     3361 ATGAACACCT ATAATTAAAC TATTCATCTA TTATTTATGA TTTTTTGTAT ATACAATATT
     3421 TCTAGTTTGT TAAAGAGAAT TAAGAAAATA AATCTCGAAA ATAATAAAGG GAAAATCAGT
     3481 TTTTGATATC AAAATTATAC ATGTCAACGA TAATACAAAA TATAATACAA ACTATAAGAT
     3541 GTTATCAGTA TTTATTATGC ATTTAGAATA AATTTTGTGT CGCCCTTATT CGACTCACTA
     3601 TAGAAGTTCC TATTCTCTAG AAAGTATAGG AACTTCACTT CATTTTATTT AAATTTGCGG
     3661 CCGCTATGTC TGATAAAAGG ATGTAACATA GGCAAGCTGC TCGTGAGTGT TGAGTACGAA
     3721 CCTTAGATCC AAATCACCCG CACCCACGGA TATACTTGCT TGAATATACA GTAGTAAGGA
     3781 GTTTGGCGCC CGTTTTTTCG AGCCCCACAC GTTTCGGTGA GTATGAGCGG CGGCAGATTC
     3841 GAGCGTTTCC GGTTTCCGCG GCTGGACGAG AGCCCATGAT GGGGGCTCCC ACCACCAGCA
     3901 ATCAGGGCCC TGATTACACA CCCACCTGTA ATGTCATGCT GTTCATCGTG GTTAATGCTG
     3961 CTGTGTGCTG TGTGTGTGTG TTGTTTGGCG CTCATTGTTG CGTTATGCAG CGTACACCAC
     4021 AATATTGGAA GCTTATTAGC CTTTCTATTT TTTCGTTTGC AAGGCTTAAC AACATTGCTG
     4081 TGGAGAGGGA TGGGGATATG GAGGCCGCTG GAGGGAGTCG GAGAGGCGTT TTGGAGCGGC
     4141 TTGGCCTGGC GCCCAGCTCG CGAAACGCAC CTAGGACCCT TTGGCACGCC GAAATGTGCC
     4201 ACTTTTCAGT CTAGTAACGC CTTACCTACG TCATTCCATG CATGCATGTT TGCGCCTTTT
     4261 TTCCCTTGCC CTTGATCGCC ACACAGTACA GTGCACTGTA CAGTGGAGGT TTTGGGGGGG
     4321 TCTTAGATGG GAGCTAAAAG CGGCCTAGCG GTACACTAGT GGGATTGTAT GGAGTGGCAT
     4381 GGAGCCTAGG TGGAGCCTGA CAGGACGCAC GACCGGCTAG CCCGTGACAG ACGATGGGTG
     4441 GCTCCTGTTG TCCACCGCGT ACAAATGTTT GGGCCAAAGT CTTGTCAGCC TTGCTTGCGA
     4501 ACCTAATTCC CAATTTTGTC ACTTCGCACC CCCATTGATC GAGCCCTAAC CCCTGCCCAT
     4561 CAGGCAATCC AATTAAGCTC GCATTGTCTG CCTTGTTTAG TTTGGCTCCT GCCCGTTTCG
     4621 GCGTCCACTT GCACAAACAC AAACAAGCAT TATATATAAG GCTCGTCTCT CCCTCCCAAC
     4681 CACACTCACT TTTTTGCCCG TCTTCCCTTG CTAACACAAA AGTCAAGAAC ACAAACAACC
     4741 ACCCCAACCC CCTTACACAC AAGACATATC TACAGCAatg ggtaaaaagc ctgaactcac
     4801 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     4861 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     4921 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     4981 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
     5041 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
     5101 actgcccgct gttctgcagc cggtcgcgga ggcaatggat gccattgctg cggccgatct
     5161 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
     5221 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
     5281 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
     5341 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
     5401 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
     5461 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
     5521 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
     5581 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
     5641 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
     5701 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
     5761 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataatcat gtaattagtt
     5821 atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt
     5881 agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg
     5941 ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt
     6001 atactgaaaa ccttgcttga gaaggttttg ggacgctcgA GCAGGCTTGG AGGCGACGTG
     6061 GCAGTGGCTC GCGGGAGGAT CGGCGGGAAA CTCCGGGATA TCCGTCGAGA GTTTACACGT
     6121 GAATGGGCAG CGCAATCCGT TGACGACGAT ACGACTGGCA AAGTAGCGAC GATACCTGCC
     6181 AGACAGGTGA CATGTGCAGG CCGCACTAAC AAGGAAACGG GCGCTGGGGG GGGCGGGCTT
     6241 CTAGACTTTG CCCTTGAACA GGAATCTAGT GGGGGCTTGT CTTTCCGCCA ATGGGGGAGC
     6301 GCCTGTTGAG CGACCGTGCA TGCTGGAACG CCAAGTGTAT GTACAGCTGG TGGTCTCGCA
     6361 GCGGTATGTG ACGGGACTTA CATCTCTCGT TTTTTCATGA CCACGTTTTC ACAGGCTCGG
     6421 AGGTACGTTA AAGTTTTGAA GGCTGCATCT GAACCGAGGT ATGGGGGAGT TTGAACAGCA
     6481 ACAGTGTTGG GGCTGAGGGG GCCAAGATCG GGGCAAGCAG AGGTCTTAGA TCAATTGTGG
     6541 GGATCCCAAA GGGCTCGTTA TCACCTTTTT CCACCCAATT CGGGTCCCAA TTGATCCACT
     6601 ACTGGCTTGC CCAAGTTACC CCAGAAATGC CGCCCCGGAT TTCTCCAAAA ACCTAATAAG
     6661 CTTCATGGAA CTTGGTGGAA GTGACTTTCT ACAGAGTGGA GAGAACCGTG GACACGTGGC
     6721 AATGGCGCTG ACCGTGTCCC CGAGCCGAAT CGACGTGAGG GGAGAACGGA GTATCTGCGG
     6781 TCATGTGACC TTCCAGAGCG GCGTCGCCAG TGTGCACGCG GTGACCCCCA GTTTGGGTCT
     6841 CTGTCACACG CATACTACCT CGGCTCTCCA CATGCTGAAC TTTATCTTTC GTGGGGATCA
     6901 TACCGAAAGT TGCAACTACC AGGTGTATAT AAAGCCTGGT AGACTCCCCC CACTTTGGAC
     6961 CTCATCCAAC CAAGACACAC AAAAATGGAC CGACTTAACA ACCTCGCCAC CCAGCTCGAG
     7021 CAGAACCCCG CCAATTCGTA GATAATGGAA TACAAATGGA TATCCAGAGT ATACACATGG
     7081 ATAGTATACA CTGACACGAC AATTCTGTAT CTC
//