LOCUS       Exported                8430 bp ds-DNA     circular SYN 08-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        270..649
                     /label=CMV enhancer
     misc_feature    279..303
                     /label=ACK14
     promoter        650..853
                     /label=CMV promoter
     misc_feature    779..803
                     /label=ACK15
     promoter        895..913
                     /label=T7 promoter
     CDS             927..5957
                     /codon_start=1
                     /label=Translation 927-5957
                     /translation="MKRTADGSEFESPKKKRKVDKKYSIGLAIGTNSVGWAVITDEYKV
                     PSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIF
                     SNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDS
                     TDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINAS
                     GVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAK
                     LQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMI
                     KRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILE
                     KMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKI
                     EKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFD
                     KNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILE
                     DIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSG
                     KTILDFLKSDGFANANFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKK
                     GILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQ
                     ILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSID
                     NKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
                     KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQ
                     FYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAK
                     VEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELE
                     NGRKRMLASARFLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYL
                     DEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFK
                     YFDTTIDRKVYRSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSKRTADGSEFEPK
                     KKRKVGSGATNFSLLKQAGDVEENPGPMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEF
                     EIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS
                     FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
                     SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSH
                     NEDYTIVEQYERAEGRHSTGGMDELYKSGGSPKKKRKV"
     CDS             927..983
                     /codon_start=1
                     /label=Translation 927-983
                     /translation="MKRTADGSEFESPKKKRKV"
     misc_feature    927..945
                     /label=QC158 (1MM)
     CDS             930..983
                     /label=bpNLS
     misc_feature    930..947
                     /label=QC199 (1MM)
     misc_feature    complement(963..993)
                     /label=QC75
     misc_feature    complement(963..983)
                     /label=anneal
     misc_feature    978..1003
                     /label=QC70
     misc_feature    978..993
                     /label=USER
     CDS             981..5084
                     /codon_start=1
                     /label=Translation 981-5084
                     /translation="VDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANANFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    984..5213
                     /label=HiFi-nCas9-NG
     CDS             984..5084
                     /label=nCas9 (D10A)
     misc_feature    984..1003
                     /label=anneal
     misc_feature    complement(1008..1023)
                     /label=ACK2207
     misc_feature    1011..1028
                     /label=ACK2206
     misc_feature    1011..1023
                     /label=USER jct
     misc_feature    1509..1533
                     /label=ACK2134
     misc_feature    2033..2057
                     /label=ACK2135
     misc_feature    2561..2585
                     /label=ACK2136
     misc_feature    complement(3038..3061)
                     /label=QC357
     misc_feature    3048..3073
                     /label=QC356
     misc_feature    3048..3061
                     /label=USER (HiFi)
     misc_feature    3051..3053
                     /label=HiFi (R691A)
     misc_feature    3082..3107
                     /label=ACK2137
     misc_feature    complement(3479..3509)
                     /label=ACK2223
     misc_feature    3501..3519
                     /label=anneal
     misc_feature    3611..3635
                     /label=ACK2138
     misc_feature    4127..4151
                     /label=ACK2139
     misc_feature    complement(4289..4309)
                     /label=ACK2143
     misc_feature    complement(4290..4313)
                     /label=ACK2221
     misc_feature    4296..4315
                     /label=ACK2142
     misc_feature    4296..4309
                     /label=USER jct 1
     misc_feature    4299..4331
                     /label=ACK2220
     misc_feature    4299..4313
                     /label=USER jct
     misc_feature    4311..4313
                     /label=L1110R
     misc_feature    complement(4363..4380)
                     /label=ACK2149
     misc_feature    4372..4388
                     /label=ACK2148
     misc_feature    4372..4380
                     /label=USER jct 4
     misc_feature    4383..4385
                     /label=D1135V
     misc_feature    complement(4610..4628)
                     /label=ACK2145
     misc_feature    4617..4653
                     /label=ACK2144a
     misc_feature    4617..4628
                     /label=USER jct 2
     misc_feature    4632..4634
                     /label=G1218R
     misc_feature    4635..4637
                     /label=E1219F
     misc_feature    4653..4678
                     /label=ACK2140
     misc_feature    complement(4939..4956)
                     /label=ACK2151
     misc_feature    4944..4963
                     /label=ACK2150
     misc_feature    4944..4956
                     /label=USER jct 5
     misc_feature    4944..4946
                     /label=A1322R
     misc_feature    complement(4966..4991)
                     /label=ACK2219
     misc_feature    complement(4969..4986)
                     /label=ACK2147
     misc_feature    4975..4996
                     /label=ACK2146
     misc_feature    4975..4986
                     /label=USER jct 3
     misc_feature    4980..5009
                     /label=ACK2218
     misc_feature    4980..4991
                     /label=USER jct 2
     misc_feature    4983..4985
                     /label=R1335V
     misc_feature    4989..4991
                     /label=T1337R
     misc_feature    complement(5032..5046)
                     /label=ACK2209
     misc_feature    5037..5052
                     /label=ACK2208
     misc_feature    5037..5046
                     /label=USER jct
     misc_feature    5057..5081
                     /label=ACK2141
     CDS             5085..5213
                     /codon_start=1
                     /label=Translation 5085-5213
                     /translation="SGGSKRTADGSEFEPKKKRKVGSGATNFSLLKQAGDVEENPGP"
     CDS             5127..5147
                     /label=SV40 NLS
     CDS             5157..5213
                     /label=P2A
     CDS             5157..5213
                     /codon_start=1
                     /label=Translation 5157-5213
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    complement(5196..5215)
                     /label=QC71
     misc_feature    5200..5229
                     /label=QC72
     misc_feature    5200..5215
                     /label=USER P2A
     CDS             5214..5921
                     /codon_start=1
                     /label=Translation 5214-5921
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5214..5921
                     /label=mCherry frag
     misc_feature    5214..5229
                     /label=F
     primer_bind     complement(5359..5377)
                     /label=mCherry-R
     primer_bind     5627..5646
                     /label=mCherry-F
     misc_feature    complement(5903..5924)
                     /label=QC73
     misc_feature    complement(5903..5921)
                     /label=anneal
     misc_feature    5908..5940
                     /label=QC74
     misc_feature    5908..5924
                     /label=USER
     CDS             5922..5957
                     /codon_start=1
                     /label=Translation 5922-5957
                     /translation="SGGSPKKKRKV"
     misc_feature    5922..5940
                     /label=anneal
     CDS             5934..5954
                     /label=SV40 NLS
     CDS             5963..5980
                     /label=6xHis
     polyA_signal    6009..6233
                     /label=bGH poly(A) signal
     misc_feature    complement(6155..6179)
                     /label=ACK64
     primer_bind     complement(6304..6320)
                     /label=M13 rev
     protein_bind    6328..6344
                     /label=lac operator
     promoter        complement(6352..6382)
                     /label=lac promoter
     misc_feature    6397..6421
                     /label=ACK21
     misc_feature    complement(6397..6421)
                     /label=ACK32
     protein_bind    6397..6418
                     /label=CAP binding site
     rep_origin      complement(6706..7294)
                     /direction=LEFT
                     /label=ori
     misc_feature    6706..6730
                     /label=ACK10
     misc_feature    7207..7231
                     /label=ACK11
     CDS             complement(7465..8325)
                     /label=AmpR
     CDS             complement(7465..8325)
                     /codon_start=1
                     /label=Translation 8325-7465
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    7706..7730
                     /label=ACK12
     misc_feature    complement(7929..7950)
                     /label=SG791
     misc_feature    7933..7958
                     /label=SG790
     misc_feature    8206..8230
                     /label=ACK13
     promoter        complement(8326..8430)
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
       61 cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg ccgctaatac
      901 gactcactat agggagagcc gccaccatga aacggacagc cgacggaagc gagttcgagt
      961 caccaaagaa gaagcggaaa gtcgacaaga agtacagcat cggcctggcc atcggcacca
     1021 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
     1081 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg
     1141 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
     1201 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
     1261 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
     1321 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
     1381 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
     1441 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
     1501 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
     1561 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
     1621 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
     1681 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
     1741 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
     1801 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1861 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1921 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1981 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     2041 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca
     2101 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     2161 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     2221 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     2281 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     2341 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     2401 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc
     2461 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     2521 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa
     2581 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca
     2641 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     2701 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     2761 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     2821 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2881 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2941 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     3001 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac gctaacttca
     3061 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     3121 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     3181 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     3241 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     3301 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     3361 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     3421 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     3481 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca
     3541 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     3601 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc
     3661 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     3721 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     3781 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga
     3841 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3901 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3961 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     4021 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     4081 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     4141 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt
     4201 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata
     4261 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc cgtcccaaga
     4321 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     4381 tcgtgagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     4441 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     4501 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     4561 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     4621 tggcctctgc ccggttcctg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     4681 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     4741 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca
     4801 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct
     4861 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta
     4921 ccctgaccaa tctgggagcc cctagggcct tcaagtactt tgacaccacc atcgaccgga
     4981 aggtgtaccg tagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     5041 gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctggc ggctcaaaaa
     5101 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga agcggagcta
     5161 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga
     5221 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     5281 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     5341 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     5401 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     5461 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     5521 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     5581 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     5641 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     5701 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     5761 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     5821 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     5881 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtctggtggt tctcccaaga
     5941 agaagaggaa agtctaaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     6001 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6061 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6121 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6181 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6241 ggcggaaaga accagctggg gctcgatacc gtcgacctct agctagagct tggcgtaatc
     6301 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6361 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6421 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6481 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6541 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6601 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6661 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6721 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6781 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6841 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6901 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6961 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7021 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7081 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7141 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7201 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7261 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7321 gtctgacact cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7381 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7441 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7501 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7561 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7621 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7681 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7741 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7801 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7861 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7921 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7981 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8041 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8101 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8161 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8221 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8281 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8341 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8401 ttagaaaaat aaacaaatag gggttccgcg
//
