LOCUS       Exported                4473 bp ds-DNA     circular SYN 12-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..249
                     /label=CMV enhancer
     misc_feature    10..34
                     /label=ACK14
     enhancer        250..380
                     /label=CMV enhancer
     promoter        381..584
                     /label=CMV promoter
     misc_feature    510..534
                     /label=ACK15
     misc_feature    599..618
                     /label=IVT_FWD
     promoter        626..644
                     /label=T7 promoter
     CDS             658..1728
                     /codon_start=1
                     /label=Translation 658-1728
                     /translation="MKRTADGSEFESPKKKRKVKSIRCKNCNKLLFKADSFDHIEIRCP
                     RCKRHIIMLNACEHPTEKHCGKREKITHSDETVRYGGGGTGGGGSAEYVRALFDFNGND
                     EEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMILVPYVEKYSGDYKDHDGDYKDHDI
                     DYKDDDDKSGSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIG
                     LHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNS
                     KRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINS
                     KRTADGSEFEPKKKRKV"
     CDS             658..897
                     /codon_start=1
                     /label=Translation 658-897
                     /translation="MKRTADGSEFESPKKKRKVKSIRCKNCNKLLFKADSFDHIEIRCP
                     RCKRHIIMLNACEHPTEKHCGKREKITHSDETVRY"
     misc_feature    661..714
                     /label=bpNLS
     misc_feature    complement(694..727)
                     /label=QC114
     misc_feature    complement(694..714)
                     /label=anneal
     misc_feature    715..897
                     /label=Com
     misc_feature    715..737
                     /label=QC113
     misc_feature    715..727
                     /label=USER
     misc_feature    complement(880..897)
                     /label=QC115
     misc_feature    886..914
                     /label=QC116
     misc_feature    886..900
                     /label=USER
     CDS             898..1176
                     /label=93aa linker
     misc_feature    complement(1156..1176)
                     /label=QC47
     misc_feature    1160..1195
                     /label=QC 146
     misc_feature    1160..1176
                     /label=USER 93aa
     misc_feature    1177..1674
                     /label=TadA** (ABE8e)
     misc_feature    1177..1195
                     /label=146 anneal
     misc_feature    complement(1659..1686)
                     /label=QC 147
     misc_feature    complement(1659..1677)
                     /label=R anneal
     misc_feature    1669..1693
                     /label=QC 148
     misc_feature    1669..1686
                     /label=USER
     misc_feature    1675..1693
                     /label=148 anneal
     misc_feature    1678..1728
                     /label=bpNLS
     misc_feature    complement(1707..1728)
                     /label=QC111 - R del
     CDS             1708..1728
                     /label=SV40 NLS
     misc_feature    1722..1749
                     /label=new anneal
                     
     misc_feature    1729..1749
                     /label=QC112 - F del
     misc_feature    1729..1748
                     /label=QC241 anneal
     CDS             1737..1754
                     /label=6xHis
     misc_feature    complement(1777..1794)
                     /label=bGH poly(A) - genewiz
     polyA_signal    1783..2007
                     /label=bGH poly(A) signal
     misc_feature    complement(1929..1953)
                     /label=ACK64
     misc_feature    complement(1963..1982)
                     /label=IVT_rev
     primer_bind     complement(2078..2094)
                     /label=M13 rev
     protein_bind    2102..2118
                     /label=lac operator
     promoter        complement(2126..2156)
                     /label=lac promoter
     misc_feature    2171..2195
                     /label=ACK21
     misc_feature    complement(2171..2195)
                     /label=ACK32
     protein_bind    2171..2192
                     /label=CAP binding site
     rep_origin      complement(2480..3068)
                     /direction=LEFT
                     /label=ori
     misc_feature    2480..2504
                     /label=ACK10
     misc_feature    2981..3005
                     /label=ACK11
     CDS             complement(3239..4099)
                     /label=AmpR
     CDS             complement(3239..4099)
                     /codon_start=1
                     /label=Translation 4099-3239
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    3321..3341
                     /label=CMV-F (genewiz)
     misc_feature    3412..3431
                     /label=T7 F (genewiz)
     misc_feature    3480..3504
                     /label=ACK12
     misc_feature    3980..4004
                     /label=ACK13
     promoter        complement(4100..4204)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcaaaagc
      721 atcagatgca agaactgcaa caagctgctg ttcaaggccg acagcttcga ccacatcgag
      781 atccggtgtc ctagatgtaa aagacacatc atcatgctga acgcctgcga gcaccccacc
      841 gagaagcact gcggcaagcg ggaaaagatc acccacagcg acgagacagt gcggtacggt
      901 ggaggaggta ccggcggtgg aggctcagca gaatacgtac gagctctgtt tgacttcaat
      961 gggaatgacg aggaggatct cccctttaag aagggcgata ttctccgcat cagagataag
     1021 cccgaagaac aatggtggaa tgccgaggat agcgaaggga aaaggggcat gattctggtg
     1081 ccatatgtgg agaaatattc cggtgactac aaagaccatg atggggatta caaagaccac
     1141 gacatcgact acaaagacga cgacgataaa tcaggttctg aggtggagtt ttcccacgag
     1201 tactggatga gacatgccct gaccctggcc aagagggcac gggatgagag ggaggtgcct
     1261 gtgggagccg tgctggtgct gaacaataga gtgatcggcg agggctggaa cagagccatc
     1321 ggcctgcacg acccaacagc ccatgccgaa attatggccc tgagacaggg cggcctggtc
     1381 atgcagaact acagactgat tgacgccacc ctgtacgtga cattcgagcc ttgcgtgatg
     1441 tgcgccggcg ccatgatcca ctctaggatc ggccgcgtgg tgtttggcgt gaggaactca
     1501 aaaagaggcg ccgcaggctc cctgatgaac gtgctgaact accccggcat gaatcaccgc
     1561 gtcgaaatta ccgagggaat cctggcagat gaatgtgccg ccctgctgtg cgatttctat
     1621 cggatgccta gacaggtgtt caatgctcag aagaaggccc agagctccat caactccaaa
     1681 agaactgccg acggcagcga attcgagccc aagaagaaga ggaaagtcta accggtcatc
     1741 atcaccatca ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     1801 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     1861 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     1921 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     1981 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     2041 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2161 agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3061 gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg aacgaaaact
     3121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4201 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
     4261 atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     4321 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     4381 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     4441 tgcttcgcga tgtacgggcc agatatacgc gtt
//
