LOCUS       Exported                4296 bp ds-DNA     circular SYN 08-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            addgene-plasmid-138491-sequence-272891.gbk A-to-G base editor.
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
     misc_feature    68
                     /label=T>G
     promoter        381..584
                     /label=CMV promoter
     primer_bind     535..555
                     /label=CMV-F
     primer_bind     626..645
                     /label=T7
     promoter        626..644
                     /label=T7 promoter
     misc_feature    complement(654..672)
                     /label=QC207
     CDS             658..1554
                     /codon_start=1
                     /label=Translation 658-1554
                     /translation="MKRTADGSEFESPKKKRKVSSEVEFSHEYWMRHALTLAKRARDER
                     EVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFE
                     PCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAAL
                     LCRFFRMPRRVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSKSIR
                     CKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEKHCGKREKITHSDETVRYGS
                     KRTADGSEFEPKKKRKV"
     CDS             658..1554
                     /codon_start=1
                     /label=Translation 658-1554
                     /translation="MKRTADGSEFESPKKKRKVSSEVEFSHEYWMRHALTLAKRARDER
                     EVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFE
                     PCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAAL
                     LCRFFRMPRRVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSKSIR
                     CKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEKHCGKREKITHSDETVRYGS
                     KRTADGSEFEPKKKRKV"
     misc_feature    661..714
                     /label=bpNLS
     misc_feature    complement(694..730)
                     /label=QC209
     CDS             694..714
                     /label=SV40 NLS
     misc_feature    complement(694..714)
                     /label=anneal
     misc_feature    715..1551
                     /label=QTC135 insert (orABE8.20)
     misc_feature    715..735
                     /label=QC 151
     misc_feature    715..730
                     /label=USER
     misc_feature    718..1215
                     /label=TadA** (ABE8.20)
     misc_feature    718..735
                     /label=151 anneal
     misc_feature    complement(1198..1217)
                     /label=150anneal 1 mm
     misc_feature    1216..1311
                     /label=32aa linker
     misc_feature    complement(1294..1324)
                     /label=QC208
     misc_feature    complement(1294..1311)
                     /label=anneal
     misc_feature    1312..1494
                     /label=Com
     CDS             1312..1494
                     /codon_start=1
                     /label=Translation 1312-1494
                     /translation="KSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEKH
                     CGKREKITHSDETVRY"
     misc_feature    1312..1334
                     /label=QC113
     misc_feature    1312..1324
                     /label=USER
     misc_feature    complement(1477..1494)
                     /label=QC115
     misc_feature    complement(1481..1497)
                     /label=QC115-Short
     misc_feature    1483..1516
                     /label=QC171
     misc_feature    1483..1497
                     /label=USER
     CDS             1495..1497
                     /codon_start=1
                     /label=Translation 1495-1497
                     /translation="G"
     misc_feature    1495..1497
                     /label=Linker
     misc_feature    1498..1516
                     /label=anneal
     misc_feature    1501..1551
                     /label=bpNLS
     misc_feature    complement(1530..1551)
                     /label=QC251 anneal
     CDS             1531..1551
                     /label=SV40 NLS
     CDS             1560..1577
                     /label=6xHis
     misc_feature    complement(1562..1581)
                     /label=R
     primer_bind     complement(1600..1617)
                     /label=BGH-rev (Genewiz)
     polyA_signal    1606..1830
                     /label=bGH poly(A) signal
     primer_bind     complement(1901..1917)
                     /label=M13 Reverse
     primer_bind     complement(1901..1917)
                     /label=M13 rev
     primer_bind     complement(1914..1936)
                     /label=M13/pUC Reverse
     protein_bind    1925..1941
                     /label=lac operator
     promoter        complement(1949..1979)
                     /label=lac promoter
     protein_bind    1994..2015
                     /label=CAP binding site
     primer_bind     complement(2132..2149)
                     /label=L4440
     rep_origin      complement(2303..2891)
                     /direction=LEFT
                     /label=ori
     primer_bind     complement(2383..2402)
                     /label=pBR322ori-F
     CDS             complement(3062..3922)
                     /label=AmpR
     CDS             complement(3062..3922)
                     /codon_start=1
                     /label=Translation 3922-3062
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3685..3704
                     /label=Amp-R
     promoter        complement(3923..4027)
                     /label=AmpR promoter
     primer_bind     complement(4106..4125)
                     /label=pRS-marker
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatattga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 aaacggacag ccgacggaag cgagttcgag tcaccaaaga agaagcggaa agtcagctct
      721 gaggtggagt tttcccatga gtactggatg agacacgcat tgactctcgc aaagagggct
      781 cgagatgaac gcgaggtgcc cgtgggggca gtactcgtgc tcaacaatcg cgtaatcggc
      841 gaaggttgga atagggcaat cggactccac gaccccactg cacatgcgga aatcatggcc
      901 cttcgacagg gagggcttgt gatgcagaat tatcgacttt atgatgcgac gctgtactcg
      961 acgtttgaac cttgcgtaat gtgcgcggga gctatgattc actcccgcat tggacgagtt
     1021 gtattcggtg ttcgcaacgc caagacgggt gccgcaggtt cactgatgga cgtgctgcat
     1081 catccaggca tgaaccaccg ggtagaaatc acagaaggca tattggcgga cgaatgtgcg
     1141 gcgctgttgt gtcgtttttt tcgcatgccc aggcgggtct ttaacgccca gaaaaaagca
     1201 caatcctcta ctgactctgg tggttcttct ggtggttcta gcggcagcga gactcccggg
     1261 acctcagagt ccgccacacc cgaaagttct ggtggttctt ctggtggttc taaaagcatc
     1321 agatgcaaga actgcaacaa gctgctgttc aaggccgaca gcttcgacca catcgagatc
     1381 cggtgtccta gatgtaaaag acacatcatc atgctgaacg cctgcgagca ccccaccgag
     1441 aagcactgcg gcaagcggga aaagatcacc cacagcgacg agacagtgcg gtacggttca
     1501 aaaagaaccg ccgacggcag cgaattcgag cccaagaaga agaggaaagt ctaaccggtc
     1561 atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     1621 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     1681 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     1741 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     1801 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     1861 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1981 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2701 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa
     2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     4081 ccgatcccct agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     4141 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     4201 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     4261 cgctgcttcg cgatgtacgg gccagatata cgcgtt
//
