LOCUS       Exported                5148 bp ds-DNA     circular SYN 12-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5148
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..249
                     /label=CMV enhancer
     enhancer        250..380
                     /label=CMV enhancer
     promoter        381..584
                     /label=CMV promoter
     misc_feature    510..534
                     /label=ACK15
     misc_feature    535..555
                     /label=CMV-F (genewiz)
     promoter        626..644
                     /label=T7 promoter
     misc_feature    complement(644..675)
                     /label=QC45*
     misc_feature    complement(644..660)
                     /label=QC43 anneal
     CDS             658..2403
                     /codon_start=1
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGSTNLSDIIEKETGKQ
                     LVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQD
                     SNGENKIKMLSGGSGGSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESD
                     ILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSKRTADGSEFEP
                     KKKRKV"
     misc_feature    658..2403
                     /label=QTC162 FRAG
     misc_feature    658..2403
                     /label=QTC162 (MCP-16-evo)
     CDS             658..1812
                     /codon_start=1
                     /label=Translation 658-1812
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGS"
     misc_feature    658..681
                     /label=QC44*
     misc_feature    658..675
                     /label=USER MCP (1)
     misc_feature    661..1047
                     /label=MS2-CP (MCP)
     misc_feature    675
                     /label=silent A>T
     misc_feature    823..825
                     /label=N55K
     misc_feature    complement(1030..1050)
                     /label=QC45*
     misc_feature    1033..1050
                     /label=USER MCP (2)
     misc_feature    1050
                     /label=SILENT A>T
     misc_feature    1051..1098
                     /label=16aa linker (SGGS)4
     misc_feature    complement(1052..1074)
                     /label=QC197
     misc_feature    1074..1075
                     /label=DEL
     misc_feature    1075..1090
                     /label=QC196
     misc_feature    complement(1085..1098)
                     /label=QC49 anneal
     misc_feature    1099..1782
                     /label=evo-rAPOBEC1
     misc_feature    1099..1120
                     /label=QC191
     misc_feature    1099..1116
                     /label=USER
     misc_feature    1783..1812
                     /label=10aa linker
     misc_feature    1795..1803
                     /label=new codons
     CDS             1813..2403
                     /codon_start=1
                     /label=Translation 1813-2403
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSGGSGGSTNLSDIIEKET
                     GKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALV
                     IQDSNGENKIKMLSGGSKRTADGSEFEPKKKRKV"
     CDS             1813..2061
                     /label=UGI
     misc_feature    1813..1837
                     /label=QC50
     misc_feature    1813..1829
                     /label=USER UGI
     misc_feature    complement(1813..1829)
                     /label=QC49
     misc_feature    complement(2049..2068)
                     /label=UGI-rev_seq
     CDS             2092..2340
                     /label=UGI
     misc_feature    2353..2403
                     /label=bpNLS
     CDS             2383..2403
                     /label=SV40 NLS
     CDS             2404..2406
                     /codon_start=1
                     /label=Translation 2404-2406
                     /translation=""
     CDS             2412..2429
                     /label=6xHis
     misc_feature    complement(2452..2469)
                     /label=bGH-R (genewiz)
     polyA_signal    2458..2682
                     /label=bGH poly(A) signal
     misc_feature    complement(2604..2628)
                     /label=ACK64
     primer_bind     complement(2753..2769)
                     /label=M13 rev
     protein_bind    2777..2793
                     /label=lac operator
     promoter        complement(2801..2831)
                     /label=lac promoter
     misc_feature    2846..2870
                     /label=ACK21
     protein_bind    2846..2867
                     /label=CAP binding site
     rep_origin      complement(3155..3743)
                     /direction=LEFT
                     /label=ori
     misc_feature    3155..3179
                     /label=ACK10
     CDS             complement(3914..4774)
                     /label=AmpR
     CDS             complement(3914..4774)
                     /codon_start=1
                     /label=Translation 4774-3914
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4775..4879)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gcaagtaact tcactcaatt tgtgcttgta gacaacggag gtaccggaga tgtaacagta
      721 gcacccagta atttcgcaaa cggcgtggcg gaatggatta gttcaaattc aagatctcag
      781 gcctataagg tcacttgctc cgtcaggcag tctagtgccc aaaagcgcaa atacacgatt
      841 aaagtagagg taccgaaggt cgctacgcaa actgttgggg gagttgaact tccagtggct
      901 gcttggcgct catacctgaa catggaactg actatcccca tattcgctac taactccgat
      961 tgtgaactca tagtaaaagc tatgcaggga ttgctcaagg atggcaaccc cattccaagt
     1021 gccatagcag cgaactccgg gatatattct tctggaggat ctagcggagg atccagtggc
     1081 ggcagcagcg gcggcagcag ttcaaagact gggcctgtcg ccgtcgatcc aaccctgcgc
     1141 cgccggattg aacctcacga gtttgaagtg ttctttgacc cccgggagct gagaaaggag
     1201 acatgcctgc tgtacgagat caactgggga ggcaggcact ccatctggag gcacacctct
     1261 cagaacacaa ataagcacgt ggaggtgaac ttcatcgaga agtttaccac agagcggtac
     1321 ttctgcccca ataccagatg tagcatcaca tggtttctga gctggtcccc ttgcggagag
     1381 tgtagcaggg ccatcaccga gttcctgtcc agatatccaa atgtgacact gtttatctac
     1441 atcgccaggc tgtatcacct ggcaaaccca aggaataggc agggcctgcg cgatctgatc
     1501 agctccggcg tgaccatcca gatcatgaca gagcaggagt ccggctactg ctggcacaac
     1561 ttcgtgaatt attctcctag caacgagtcc cactggccta ggtacccaca cctgtgggtg
     1621 cgcctgtacg tgctggagct gtattgcatc atcctgggcc tgcccccttg tctgaatatc
     1681 ctgcggagaa agcagagcca gctgacctcc tttacaatcg ccctgcagtc ttgtcactat
     1741 cagaggctgc caccccacat cctgtgggcc acaggcctga agtctggcgg atctggaggc
     1801 tccggcggca gcactaatct gagcgacatc attgagaagg agactgggaa acagctggtc
     1861 attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca
     1921 gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg
     1981 ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc
     2041 gagaataaga tcaagatgct gagcggagga tccggaggat ctggaggcag caccaacctg
     2101 tctgacatca tcgagaagga gacaggcaag cagctggtca tccaggagag catcctgatg
     2161 ctgcccgaag aagtcgaaga agtgatcgga aacaagcctg agagcgatat cctggtccat
     2221 accgcctacg acgagagtac cgacgaaaat gtgatgctgc tgacatccga cgccccagag
     2281 tataagccct gggctctggt catccaggat tccaacggag agaacaaaat caaaatgctg
     2341 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaaaaggaaa
     2401 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     2461 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     2521 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     2581 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     2641 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     2701 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     2761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2821 taaagtgtaa agcctaggat gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2941 gcgcgggaag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc
     3781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     4921 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
     4981 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     5041 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     5101 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
