LOCUS       Exported                6348 bp ds-DNA     circular SYN 08-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6348
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..249
                     /label=CMV enhancer
     misc_feature    93
                     /label=G>A
     enhancer        250..380
                     /label=CMV enhancer
     promoter        381..584
                     /label=CMV promoter
     misc_feature    510..534
                     /label=ACK15
     misc_feature    535..555
                     /label=CMV-F (genewiz)
     promoter        626..644
                     /label=T7 promoter
     misc_feature    complement(644..675)
                     /label=QC45*
     misc_feature    complement(644..660)
                     /label=QC43 anneal
     CDS             658..3606
                     /codon_start=1
                     /label=Translation 658-3606
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGSTNLSDIIEKETGKQ
                     LVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQD
                     SNGENKIKMLSGGSGGSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESD
                     ILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSKRTADGSEFEP
                     KKKRKVGSGATNFSLLKQAGDVEENPGPGSGEGRGSLLTCGDVEENPGPMKRTADGSEF
                     ESPKKKRKVKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEKHCGKRE
                     KITHSDETVRYGGGGTGGGGSAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWN
                     AEDSEGKRGMILVPYVEKYSGDYKDHDGDYKDHDIDYKDDDDKSGSEVEFSHEYWMRHA
                     LTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYR
                     LIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEIT
                     EGILADECAALLCDFYRMPRQVFNAQKKAQSSINSKRTADGSEFEPKKKRKV"
     misc_feature    658..2403
                     /label=QTC162 (MCP-16-evo)
     CDS             658..1812
                     /codon_start=1
                     /label=Translation 658-1812
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGS"
     misc_feature    658..681
                     /label=QC44*
     misc_feature    658..675
                     /label=USER MCP (1)
     misc_feature    661..1047
                     /label=MS2-CP (MCP)
     misc_feature    675
                     /label=silent A>T
     misc_feature    823..825
                     /label=N55K
     misc_feature    complement(1030..1050)
                     /label=QC45*
     misc_feature    1033..1050
                     /label=USER MCP (2)
     misc_feature    1050
                     /label=SILENT A>T
     misc_feature    1051..1098
                     /label=16aa linker (SGGS)4
     misc_feature    complement(1052..1074)
                     /label=QC197
     misc_feature    1074..1075
                     /label=DEL
     misc_feature    1075..1090
                     /label=QC196
     misc_feature    complement(1085..1098)
                     /label=QC49 anneal
     misc_feature    1099..1782
                     /label=evo-rAPOBEC1
     misc_feature    1099..1120
                     /label=QC191
     misc_feature    1099..1116
                     /label=USER
     misc_feature    1783..1812
                     /label=10aa linker
     misc_feature    1795..1803
                     /label=new codons
     misc_feature    1799..1821
                     /label=QC194
     CDS             1813..2403
                     /codon_start=1
                     /label=Translation 1813-2403
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSGGSGGSTNLSDIIEKET
                     GKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALV
                     IQDSNGENKIKMLSGGSKRTADGSEFEPKKKRKV"
     CDS             1813..2061
                     /label=UGI
     misc_feature    1813..1837
                     /label=QC50
     misc_feature    1813..1829
                     /label=USER UGI
     misc_feature    complement(2049..2068)
                     /label=UGI-rev_seq
     CDS             2092..2340
                     /label=UGI
     misc_feature    2353..2403
                     /label=bpNLS
     CDS             2383..2403
                     /label=SV40 NLS
     CDS             2404..2469
                     /label=P2A
     CDS             2413..2469
                     /codon_start=1
                     /label=Translation 2413-2469
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2413..2437
                     /label=ACK2210
     misc_feature    complement(2451..2478)
                     /label=QC291 (R-BB/CBE)
     misc_feature    complement(2451..2469)
                     /label=anneal
     misc_feature    complement(2466..2518)
                     /label=Rev-T2A
     primer_bind     complement(2466..2518)
                     /label=R
     misc_feature    2466..2478
                     /label=USER
     CDS             2470..2532
                     /codon_start=1
                     /label=Translation 2470-2532
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             2470..2532
                     /label=T2A
     misc_feature    2478
                     /label=silent mut
                     /label=silent mut.
     misc_feature    2479..2534
                     /label=Fwd-T2A
     primer_bind     2479..2534
                     /label=F
     misc_feature    2519..2551
                     /label=QC288 (F-ABE)
     misc_feature    2519..2534
                     /label=USER
     misc_feature    2533..3603
                     /label=apt-ABE8e
     CDS             2533..3603
                     /codon_start=1
                     /label=Translation 2533-3603
                     /translation="MKRTADGSEFESPKKKRKVKSIRCKNCNKLLFKADSFDHIEIRCP
                     RCKRHIIMLNACEHPTEKHCGKREKITHSDETVRYGGGGTGGGGSAEYVRALFDFNGND
                     EEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMILVPYVEKYSGDYKDHDGDYKDHDI
                     DYKDDDDKSGSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIG
                     LHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNS
                     KRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINS
                     KRTADGSEFEPKKKRKV"
     misc_feature    2533..3579
                     /label=QTC126 (8e)
     misc_feature    2533..2551
                     /label=anneal ABE
     misc_feature    2536..2589
                     /label=bpNLS
     misc_feature    2590..2772
                     /label=Com
     misc_feature    2590..2602
                     /label=USER
     misc_feature    complement(2755..2772)
                     /label=QC115
     misc_feature    2761..2789
                     /label=QC116
     misc_feature    2761..2775
                     /label=USER
     CDS             2773..3051
                     /label=93aa linker
     misc_feature    complement(3031..3051)
                     /label=QC47
     misc_feature    3035..3070
                     /label=QC 146
     misc_feature    3035..3051
                     /label=USER 93aa
     misc_feature    3052..3549
                     /label=TadA(8e)
                     
     misc_feature    3052..3070
                     /label=146 anneal
     misc_feature    3553..3603
                     /label=bpNLS
     misc_feature    complement(3555..3579)
                     /label=QC289
     misc_feature    complement(3555..3570)
                     /label=anneal ABE
     misc_feature    3561
                     /label=not in QTC135
     misc_feature    join(3566..6348,1..2478)
                     /label=apt-CBE-SL3 BB
     misc_feature    3566..3608
                     /label=QC290 (F-BB)
     misc_feature    3566..3579
                     /label=USER
     misc_feature    3571..3573
                     /label=new codon
     misc_feature    3576
                     /label=new codon
     misc_feature    3579
                     /label=new codon
     misc_feature    3582
                     /label=new codon
     misc_feature    3583..3608
                     /label=anneal BB
     CDS             3583..3603
                     /label=SV40 NLS
     CDS             3604..3606
                     /codon_start=1
                     /label=Translation 3604-3606
                     /translation=""
     CDS             3612..3629
                     /label=6xHis
     misc_feature    complement(3652..3669)
                     /label=bGH-R (genewiz)
     polyA_signal    3658..3882
                     /label=bGH poly(A) signal
     misc_feature    complement(3804..3828)
                     /label=ACK64
     primer_bind     complement(3953..3969)
                     /label=M13 rev
     protein_bind    3977..3993
                     /label=lac operator
     promoter        complement(4001..4031)
                     /label=lac promoter
     misc_feature    4046..4070
                     /label=ACK21
     protein_bind    4046..4067
                     /label=CAP binding site
     rep_origin      complement(4355..4943)
                     /direction=LEFT
                     /label=ori
     misc_feature    4355..4379
                     /label=ACK10
     CDS             complement(5114..5974)
                     /label=AmpR
     CDS             complement(5114..5974)
                     /codon_start=1
                     /label=Translation 5974-5114
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5975..6079)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cgataaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gcaagtaact tcactcaatt tgtgcttgta gacaacggag gtaccggaga tgtaacagta
      721 gcacccagta atttcgcaaa cggcgtggcg gaatggatta gttcaaattc aagatctcag
      781 gcctataagg tcacttgctc cgtcaggcag tctagtgccc aaaagcgcaa atacacgatt
      841 aaagtagagg taccgaaggt cgctacgcaa actgttgggg gagttgaact tccagtggct
      901 gcttggcgct catacctgaa catggaactg actatcccca tattcgctac taactccgat
      961 tgtgaactca tagtaaaagc tatgcaggga ttgctcaagg atggcaaccc cattccaagt
     1021 gccatagcag cgaactccgg gatatattct tctggaggat ctagcggagg atccagtggc
     1081 ggcagcagcg gcggcagcag ttcaaagact gggcctgtcg ccgtcgatcc aaccctgcgc
     1141 cgccggattg aacctcacga gtttgaagtg ttctttgacc cccgggagct gagaaaggag
     1201 acatgcctgc tgtacgagat caactgggga ggcaggcact ccatctggag gcacacctct
     1261 cagaacacaa ataagcacgt ggaggtgaac ttcatcgaga agtttaccac agagcggtac
     1321 ttctgcccca ataccagatg tagcatcaca tggtttctga gctggtcccc ttgcggagag
     1381 tgtagcaggg ccatcaccga gttcctgtcc agatatccaa atgtgacact gtttatctac
     1441 atcgccaggc tgtatcacct ggcaaaccca aggaataggc agggcctgcg cgatctgatc
     1501 agctccggcg tgaccatcca gatcatgaca gagcaggagt ccggctactg ctggcacaac
     1561 ttcgtgaatt attctcctag caacgagtcc cactggccta ggtacccaca cctgtgggtg
     1621 cgcctgtacg tgctggagct gtattgcatc atcctgggcc tgcccccttg tctgaatatc
     1681 ctgcggagaa agcagagcca gctgacctcc tttacaatcg ccctgcagtc ttgtcactat
     1741 cagaggctgc caccccacat cctgtgggcc acaggcctga agtctggcgg atctggaggc
     1801 tccggcggca gcactaatct gagcgacatc attgagaagg agactgggaa acagctggtc
     1861 attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca
     1921 gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg
     1981 ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc
     2041 gagaataaga tcaagatgct gagcggagga tccggaggat ctggaggcag caccaacctg
     2101 tctgacatca tcgagaagga gacaggcaag cagctggtca tccaggagag catcctgatg
     2161 ctgcccgaag aagtcgaaga agtgatcgga aacaagcctg agagcgatat cctggtccat
     2221 accgcctacg acgagagtac cgacgaaaat gtgatgctgc tgacatccga cgccccagag
     2281 tataagccct gggctctggt catccaggat tccaacggag agaacaaaat caaaatgctg
     2341 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     2401 gtcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     2461 cctggacctg gcagtggtga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     2521 aatcctggcc caatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     2581 cggaaagtca aaagcatcag atgcaagaac tgcaacaagc tgctgttcaa ggccgacagc
     2641 ttcgaccaca tcgagatccg gtgtcctaga tgtaaaagac acatcatcat gctgaacgcc
     2701 tgcgagcacc ccaccgagaa gcactgcggc aagcgggaaa agatcaccca cagcgacgag
     2761 acagtgcggt acggtggagg aggtaccggc ggtggaggct cagcagaata cgtacgagct
     2821 ctgtttgact tcaatgggaa tgacgaggag gatctcccct ttaagaaggg cgatattctc
     2881 cgcatcagag ataagcccga agaacaatgg tggaatgccg aggatagcga agggaaaagg
     2941 ggcatgattc tggtgccata tgtggagaaa tattccggtg actacaaaga ccatgatggg
     3001 gattacaaag accacgacat cgactacaaa gacgacgacg ataaatcagg ttctgaggtg
     3061 gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag ggcacgggat
     3121 gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat cggcgagggc
     3181 tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat ggccctgaga
     3241 cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta cgtgacattc
     3301 gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg cgtggtgttt
     3361 ggcgtgagga actcaaaaag aggcgccgca ggctccctga tgaacgtgct gaactacccc
     3421 ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg tgccgccctg
     3481 ctgtgcgatt tctatcggat gcctagacag gtgttcaatg ctcagaagaa ggcccagagc
     3541 tccatcaact ccaaaagaac tgccgacggc tctgagtttg aacccaagaa gaaaaggaaa
     3601 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     3661 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3721 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3781 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3841 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3901 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     3961 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4021 taaagtgtaa agcctaggat gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4081 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4141 gcgcgggaag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4201 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4261 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc
     4981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5401 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     5461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5701 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     5761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     6121 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
     6181 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     6241 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     6301 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
