LOCUS       Exported                6171 bp ds-DNA     circular SYN 08-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..249
                     /label=CMV enhancer
     enhancer        250..380
                     /label=CMV enhancer
     promoter        381..584
                     /label=CMV promoter
     misc_feature    510..534
                     /label=ACK15
     misc_feature    535..555
                     /label=CMV-F (genewiz)
     promoter        626..644
                     /label=T7 promoter
     misc_feature    complement(644..675)
                     /label=QC45*
     misc_feature    complement(644..660)
                     /label=QC43 anneal
     CDS             658..3429
                     /codon_start=1
                     /label=Translation 658-3429
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGSTNLSDIIEKETGKQ
                     LVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQD
                     SNGENKIKMLSGGSGGSGGSTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESD
                     ILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSKRTADGSEFEP
                     KKKRKVGSGATNFSLLKQAGDVEENPGPGSGEGRGSLLTCGDVEENPGPMKRTADGSEF
                     ESPKKKRKVSSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIG
                     LHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFEPCVMCAGAMIHSRIGRVVFGVRNA
                     KTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLCRFFRMPRRVFNAQKKAQSSTDS
                     GGSSGGSSGSETPGTSESATPESSGGSSGGSKSIRCKNCNKLLFKADSFDHIEIRCPRC
                     KRHIIMLNACEHPTEKHCGKREKITHSDETVRYGSKRTADGSEFEPKKKRKV"
     misc_feature    658..2403
                     /label=QTC162 (MCP-16-evo)
     CDS             658..1812
                     /codon_start=1
                     /label=Translation 658-1812
                     /translation="MASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKV
                     TCSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCEL
                     IVKAMQGLLKDGNPIPSAIAANSGIYSSGGSSGGSSGGSSGGSSSKTGPVAVDPTLRRR
                     IEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYF
                     CPNTRCSITWFLSWSPCGECSRAITEFLSRYPNVTLFIYIARLYHLANPRNRQGLRDLI
                     SSGVTIQIMTEQESGYCWHNFVNYSPSNESHWPRYPHLWVRLYVLELYCIILGLPPCLN
                     ILRRKQSQLTSFTIALQSCHYQRLPPHILWATGLKSGGSGGSGGS"
     misc_feature    658..681
                     /label=QC44*
     misc_feature    658..675
                     /label=USER MCP (1)
     misc_feature    661..1047
                     /label=MS2-CP (MCP)
     misc_feature    675
                     /label=silent A>T
     misc_feature    823..825
                     /label=N55K
     misc_feature    complement(1030..1050)
                     /label=QC45*
     misc_feature    1033..1050
                     /label=USER MCP (2)
     misc_feature    1050
                     /label=SILENT A>T
     misc_feature    1051..1098
                     /label=16aa linker (SGGS)4
     misc_feature    complement(1052..1074)
                     /label=QC197
     misc_feature    1074..1075
                     /label=DEL
     misc_feature    1075..1090
                     /label=QC196
     misc_feature    complement(1085..1098)
                     /label=QC49 anneal
     misc_feature    1099..1782
                     /label=evo-rAPOBEC1
     misc_feature    1099..1120
                     /label=QC191
     misc_feature    1099..1116
                     /label=USER
     misc_feature    1783..1812
                     /label=10aa linker
     misc_feature    1795..1803
                     /label=new codons
     CDS             1813..2403
                     /codon_start=1
                     /label=Translation 1813-2403
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKMLSGGSGGSGGSTNLSDIIEKET
                     GKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALV
                     IQDSNGENKIKMLSGGSKRTADGSEFEPKKKRKV"
     CDS             1813..2061
                     /label=UGI
     misc_feature    1813..1837
                     /label=QC50
     misc_feature    1813..1829
                     /label=USER UGI
     misc_feature    complement(1813..1829)
                     /label=QC49
     misc_feature    complement(2049..2068)
                     /label=UGI-rev_seq
     CDS             2092..2340
                     /label=UGI
     misc_feature    2353..2403
                     /label=bpNLS
     CDS             2383..2403
                     /label=SV40 NLS
     CDS             2404..2469
                     /label=P2A
     CDS             2413..2469
                     /codon_start=1
                     /label=Translation 2413-2469
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    2413..2437
                     /label=ACK2210
     misc_feature    complement(2451..2478)
                     /label=QC291 (R-BB/CBE)
     misc_feature    complement(2451..2469)
                     /label=anneal
     misc_feature    complement(2466..2518)
                     /label=Rev-T2A
     primer_bind     complement(2466..2518)
                     /label=R
     misc_feature    2466..2478
                     /label=USER
     CDS             2470..2533
                     /codon_start=1
                     /label=Translation 2470-2533
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             2470..2532
                     /label=T2A
     misc_feature    2478
                     /label=silent mut
                     /label=silent mut.
     misc_feature    2479..2534
                     /label=Fwd-T2A
     misc_feature    2519..3402
                     /label=QTC135 (8.20)
     misc_feature    2519..2551
                     /label=QC288 (ABE-F)
     misc_feature    2519..2534
                     /label=USER
     CDS             2533..3429
                     /codon_start=1
                     /label=Translation 2533-3429
                     /translation="MKRTADGSEFESPKKKRKVSSEVEFSHEYWMRHALTLAKRARDER
                     EVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLYDATLYSTFE
                     PCVMCAGAMIHSRIGRVVFGVRNAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAAL
                     LCRFFRMPRRVFNAQKKAQSSTDSGGSSGGSSGSETPGTSESATPESSGGSSGGSKSIR
                     CKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTEKHCGKREKITHSDETVRYGS
                     KRTADGSEFEPKKKRKV"
     misc_feature    2536..2589
                     /label=bpNLS
     misc_feature    complement(2569..2605)
                     /label=QC209
     CDS             2569..2589
                     /label=SV40 NLS
     misc_feature    2590..2610
                     /label=QC 151
     misc_feature    2590..2605
                     /label=USER
     misc_feature    2593..3090
                     /label=TadA** (ABE8.20)
     misc_feature    complement(3073..3092)
                     /label=150anneal 1 mm
     misc_feature    3091..3186
                     /label=32aa linker
     misc_feature    complement(3169..3199)
                     /label=QC208
     misc_feature    complement(3169..3186)
                     /label=anneal
     misc_feature    3187..3369
                     /label=Com
     misc_feature    3187..3209
                     /label=QC113
     misc_feature    3187..3199
                     /label=USER
     misc_feature    complement(3352..3369)
                     /label=QC115
     misc_feature    3370..3372
                     /label=Linker
     misc_feature    3376..3426
                     /label=bpNLS
     misc_feature    complement(3378..3402)
                     /label=QC289
     misc_feature    complement(3378..3393)
                     /label=anneal ABE
     misc_feature    3384
                     /label=not in QTC135
     misc_feature    3389..3431
                     /label=QC290 (F-BB)
     misc_feature    3389..3402
                     /label=USER
     misc_feature    3394..3396
                     /label=new codon
     misc_feature    3399
                     /label=new codon
     misc_feature    3402
                     /label=new codon
     misc_feature    3405
                     /label=new codon
     misc_feature    3406..3431
                     /label=anneal BB
     CDS             3406..3426
                     /label=SV40 NLS
     CDS             3435..3452
                     /label=6xHis
     misc_feature    join(3453..6171,1..2478)
                     /label=QTC162 BB
     misc_feature    complement(3475..3492)
                     /label=bGH-R (genewiz)
     polyA_signal    3481..3705
                     /label=bGH poly(A) signal
     primer_bind     complement(3776..3792)
                     /label=M13 rev
     protein_bind    3800..3816
                     /label=lac operator
     promoter        complement(3824..3854)
                     /label=lac promoter
     misc_feature    3869..3893
                     /label=ACK21
     protein_bind    3869..3890
                     /label=CAP binding site
     rep_origin      complement(4178..4766)
                     /direction=LEFT
                     /label=ori
     misc_feature    4178..4202
                     /label=ACK10
     CDS             complement(4937..5797)
                     /label=AmpR
     CDS             complement(4937..5797)
                     /codon_start=1
                     /label=Translation 5797-4937
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5798..5902)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 gcaagtaact tcactcaatt tgtgcttgta gacaacggag gtaccggaga tgtaacagta
      721 gcacccagta atttcgcaaa cggcgtggcg gaatggatta gttcaaattc aagatctcag
      781 gcctataagg tcacttgctc cgtcaggcag tctagtgccc aaaagcgcaa atacacgatt
      841 aaagtagagg taccgaaggt cgctacgcaa actgttgggg gagttgaact tccagtggct
      901 gcttggcgct catacctgaa catggaactg actatcccca tattcgctac taactccgat
      961 tgtgaactca tagtaaaagc tatgcaggga ttgctcaagg atggcaaccc cattccaagt
     1021 gccatagcag cgaactccgg gatatattct tctggaggat ctagcggagg atccagtggc
     1081 ggcagcagcg gcggcagcag ttcaaagact gggcctgtcg ccgtcgatcc aaccctgcgc
     1141 cgccggattg aacctcacga gtttgaagtg ttctttgacc cccgggagct gagaaaggag
     1201 acatgcctgc tgtacgagat caactgggga ggcaggcact ccatctggag gcacacctct
     1261 cagaacacaa ataagcacgt ggaggtgaac ttcatcgaga agtttaccac agagcggtac
     1321 ttctgcccca ataccagatg tagcatcaca tggtttctga gctggtcccc ttgcggagag
     1381 tgtagcaggg ccatcaccga gttcctgtcc agatatccaa atgtgacact gtttatctac
     1441 atcgccaggc tgtatcacct ggcaaaccca aggaataggc agggcctgcg cgatctgatc
     1501 agctccggcg tgaccatcca gatcatgaca gagcaggagt ccggctactg ctggcacaac
     1561 ttcgtgaatt attctcctag caacgagtcc cactggccta ggtacccaca cctgtgggtg
     1621 cgcctgtacg tgctggagct gtattgcatc atcctgggcc tgcccccttg tctgaatatc
     1681 ctgcggagaa agcagagcca gctgacctcc tttacaatcg ccctgcagtc ttgtcactat
     1741 cagaggctgc caccccacat cctgtgggcc acaggcctga agtctggcgg atctggaggc
     1801 tccggcggca gcactaatct gagcgacatc attgagaagg agactgggaa acagctggtc
     1861 attcaggagt ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca
     1921 gagtctgaca tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg
     1981 ctgacctctg acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc
     2041 gagaataaga tcaagatgct gagcggagga tccggaggat ctggaggcag caccaacctg
     2101 tctgacatca tcgagaagga gacaggcaag cagctggtca tccaggagag catcctgatg
     2161 ctgcccgaag aagtcgaaga agtgatcgga aacaagcctg agagcgatat cctggtccat
     2221 accgcctacg acgagagtac cgacgaaaat gtgatgctgc tgacatccga cgccccagag
     2281 tataagccct gggctctggt catccaggat tccaacggag agaacaaaat caaaatgctg
     2341 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     2401 gtcggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt ggaggagaac
     2461 cctggacctg gcagtggtga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     2521 aatcctggcc caatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     2581 cggaaagtca gctctgaggt ggagttttcc catgagtact ggatgagaca cgcattgact
     2641 ctcgcaaaga gggctcgaga tgaacgcgag gtgcccgtgg gggcagtact cgtgctcaac
     2701 aatcgcgtaa tcggcgaagg ttggaatagg gcaatcggac tccacgaccc cactgcacat
     2761 gcggaaatca tggcccttcg acagggaggg cttgtgatgc agaattatcg actttatgat
     2821 gcgacgctgt actcgacgtt tgaaccttgc gtaatgtgcg cgggagctat gattcactcc
     2881 cgcattggac gagttgtatt cggtgttcgc aacgccaaga cgggtgccgc aggttcactg
     2941 atggacgtgc tgcatcatcc aggcatgaac caccgggtag aaatcacaga aggcatattg
     3001 gcggacgaat gtgcggcgct gttgtgtcgt ttttttcgca tgcccaggcg ggtctttaac
     3061 gcccagaaaa aagcacaatc ctctactgac tctggtggtt cttctggtgg ttctagcggc
     3121 agcgagactc ccgggacctc agagtccgcc acacccgaaa gttctggtgg ttcttctggt
     3181 ggttctaaaa gcatcagatg caagaactgc aacaagctgc tgttcaaggc cgacagcttc
     3241 gaccacatcg agatccggtg tcctagatgt aaaagacaca tcatcatgct gaacgcctgc
     3301 gagcacccca ccgagaagca ctgcggcaag cgggaaaaga tcacccacag cgacgagaca
     3361 gtgcggtacg gttcaaaaag aactgccgac ggctctgagt ttgaacccaa gaagaaaagg
     3421 aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     3481 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     3541 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     3601 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     3661 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     3721 gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     3781 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3841 gcataaagtg taaagcctag gatgcctaat gagtgagcta actcacatta attgcgttgc
     3901 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3961 aacgcgcggg aagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4021 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4081 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4141 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4201 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4261 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4321 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4381 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4441 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4501 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4561 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4621 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4681 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4741 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca
     4801 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4861 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4921 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4981 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5041 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5101 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5161 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5221 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5281 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5341 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5401 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5461 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5521 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5581 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5641 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5701 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5761 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5821 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5881 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     5941 cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg
     6001 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     6061 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     6121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt t
//
