LOCUS       Exported                2296 bp ds-DNA     circular SYN 12-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Apr 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    64..85
                     /label=anneal
     misc_feature    73..390
                     /label=U6 promoter
     primer_bind     249..268
                     /label=ACK365
     misc_feature    313..332
                     /label=universal U6
     misc_feature    complement(369..390)
                     /label=universal reverse
     misc_feature    391..407
                     /label=cut out (ACCG)
     misc_feature    408..503
                     /label=sgRNA
     misc_feature    420..423
                     /label=TL
     misc_feature    460..463
                     /label=SL2
     misc_feature    475..497
                     /label=MS2-SL insert
     misc_feature    477..495
                     /label=MS2 (C-var)
     misc_feature    complement(532..555)
                     /label=anneal
     misc_feature    548..1221
                     /label=pUC origin
     primer_bind     770..789
                     /label=primer_bind
     primer_bind     1270..1289
                     /label=primer_bind
     misc_feature    complement(1366..2226)
                     /label=ampR
     primer_bind     1772..1791
                     /label=primer_bind
     promoter        complement(2268..2296)
                     /label=promoter
ORIGIN
        1 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
       61 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
      121 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      181 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      241 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      301 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      361 ttatatatct tgtggaaagg acgaaacacc ggggtcttcg agaagacgtt ttagagctag
      421 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accggcacat
      481 gaggatcacc catgtgccgg tgcttttttt aagcttgggc cgctcgaggt acctctctac
      541 atatgacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      601 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      661 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      721 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      781 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      841 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      901 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      961 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1021 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1081 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1141 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1201 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1261 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1321 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1381 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1441 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1501 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1561 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1621 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1681 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1741 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1801 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1861 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1921 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1981 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2041 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2101 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2161 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2221 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2281 cggatacata tttgaa
//
