LOCUS       pRSET-MagLOV        3263 bp DNA     circular SYN 29-MAY-2024
DEFINITION  Bacterial vector for high-level expression of proteins with a 
            cleavable 6xHis tag. For other reading frames, use pRSET A or pRSET 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Sequence Label: pRSET B - MagLOV
FEATURES             Location/Qualifiers
     source          1..3263
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    84..90
                     /label=RBS
                     /note="ribosome binding site"
     CDS             100..102
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation="M"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             133..141
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MAS"
     CDS             142..576
                     /codon_start=1
                     /label=MagLOV (AsLOV2 450P 496V 513K 528K 540M)
                     /translation="MLATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILG
                     RNPRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHVQPMRDQKGDV
                     QYFIGVKLDGTEHVRDAAEREKVMLIKKTAENIMEAAKEL"
     misc_feature    577..614
                     /label=MCS
                     /note="multiple cloning site"
     terminator      678..725
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      822..1277
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1303..1407
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1408..1476,1477..2268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2439..3027
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
      121 catcatcatg gtatggctag catgcttgct actactttag aacgtataga gaaaaacttc
      181 gtgatcacgg acccgagact acctgacaac cctataattt ttgcaagtga ctcattcctt
      241 cagttgactg agtattctag ggaagagatt ctagggagaa atcctagatt cttgcaagga
      301 ccagaaactg accgtgccac tgtgaggaaa atcagggatg cgatcgacaa ccaaaccgag
      361 gtgacagtgc agctaataaa ttacactaaa tctggcaaga agttctggaa cctatttcat
      421 gtgcaaccca tgagagacca aaaaggagac gtacagtact tcataggggt aaagttggat
      481 ggtactgagc atgttagaga cgcggcagaa cgtgaaaagg ttatgttaat aaaaaagacc
      541 gctgaaaaca taatggaagc ggcaaaggag ttgtaactcg agatctgcag ctggtaccat
      601 ggaattcgaa gcttgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      661 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      721 ttttgctgaa aggaggaact atatccggat ctggcgtaat agcgaagagg cccgcaccga
      781 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1021 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     1141 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     1201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     1261 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1321 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1381 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     1441 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     1501 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     1561 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1621 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     1681 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1741 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1801 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1861 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1921 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1981 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2041 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     2101 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     2161 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     2221 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     2281 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     2341 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     2401 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     2461 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     2521 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     2581 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2641 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2701 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2761 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2821 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2881 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2941 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3001 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3061 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3121 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3181 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     3241 cgttggccga ttcattaatg cag
//
