LOCUS       pOCC121-MBP-mono        9750 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...18-hkp61ab
VERSION     urn.local...18-hkp61ab
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    38..57
                     /standard_name="AP00102-pOM1-seq.F1"
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    126..151
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pOCC295-fw"
     misc_feature    174..201
                     /standard_name="POEM1-F primer"
     misc_feature    174..196
                     /standard_name="AP00103-pOM1-seq.F2"
     exon            224..1366
                     /standard_name="MBP from pMAL-c5G"
     misc_feature    complement(286..308)
                     /standard_name="AP0267-MBP-nR"
     misc_feature    1289..1309
                     /standard_name="AP0268-MBP-cF"
     misc_feature    1379..1402
                     /standard_name="PreScission(1)"
     misc_feature    join(1403..2020,2024..2119)
                     /standard_name="eGFP mammalian"
     misc_feature    1403..1423
                     /standard_name="AP0015.hGFP/Cherry-nF"
     misc_feature    complement(1403..1423)
                     /standard_name="AP0017.hGFP/Cherry-nR"
     misc_feature    1403..2119
                     /note="Geneious type: ORF"
                     /standard_name="monoGFP"
     misc_feature    1707..1726
                     /standard_name="AP0023.hGFP-mid-F"
     misc_feature    complement(1805..1824)
                     /standard_name="AP0024.hGFP-mid-R"
     misc_feature    2021..2023
                     /standard_name="A206K"
     misc_feature    2099..2119
                     /standard_name="AP0016.hGFP/Cherry-cF"
     misc_feature    complement(2099..2119)
                     /standard_name="AP0018.hGFP/Cherry-cR"
     misc_feature    2120..2149
                     /locus_tag="linker"
                     /standard_name="linker; misc"
     misc_feature    2150..5022
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    2152..5020
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of NotI/AscI Tif4631 optimized
                     (IDT) no STOP - Fragment 2 (concatenated sequence 2)"
     misc_feature    <2152..2158
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    2152..2155
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2152..5020
                     /note="Geneious type: ORF"
                     /standard_name="Tif4631"
     misc_feature    2159..2404
                     /note="Geneious type: domain"
                     /standard_name="RNA"
     misc_feature    2159..5014
                     /note="Geneious type: Chain"
                     /Note="Eukaryotic initiation factor 4F subunit p150"
                     /standard_name="Chain"
     misc_feature    2441..2491
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box1 "
     misc_feature    2540..2608
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box2"
     misc_feature    2672..2734
                     /standard_name="Cappalletti_peptide"
     misc_feature    2675..2758
                     /note="Geneious type: Compositional bias"
                     /Note="Pro/Ser/Thr-rich"
                     /standard_name="Compositional bias"
     misc_feature    2720..3055
                     /note="Geneious type: region"
                     /Note="Interaction with PAB1"
                     /standard_name="Interaction with Pab1"
     misc_feature    2735..2770
                     /standard_name="Cappalletti_peptide"
     misc_feature    2750..2830
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box3"
     misc_feature    2795..2806
                     /note="Geneious type: site"
                     /standard_name="Interaction with Pab1"
     misc_feature    2798..2809
                     /note="Geneious type: Mutagenesis"
                     /Note="In TIF4631-213; abolishes interaction with PAB1 and
                     inhibits poly(A)-dependent translation. KLRK->AAAA"
                     /standard_name="Mutagenesis"
     misc_feature    3155..3199
                     /standard_name="Cappalletti_peptide"
     misc_feature    3281..3310
                     /note="Geneious type: Compositional bias"
                     /Note="Ala/Glu-rich"
                     /standard_name="Compositional bias"
     misc_feature    3329..3628
                     /note="Geneious type: region"
                     /standard_name="eIF4E binding "
     misc_feature    3389..3415
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3518..3550
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3560..3595
                     /standard_name="Cappalletti_peptide"
     misc_feature    3569..3586
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3620..3817
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_feature    3626..3784
                     /note="Geneious type: domain"
                     /standard_name="RNA (Arg/Ser rich)"
     misc_feature    3782..4807
                     /note="Geneious type: region"
                     /standard_name="Tif1/2 (eIF4A) binding"
     misc_feature    3918..3929
                     /note="Geneious type: Mutagenesis"
                     /Note="In TIF4631-213; abolishes interaction with PAB1 and
                     inhibits poly(A)-dependent translation. KLRK->AAAA"
                     /standard_name="Mutagenesis"
     misc_feature    3965..4003
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3977..4708
                     /note="Geneious type: domain"
                     /Note="MIF4G"
                     /standard_name="MIF4G (rich in alpha-helices)"
     misc_feature    4022..4060
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4061..4072
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4085..4132
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4145..4186
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4232..4285
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4355..4423
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4439..4486
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4499..4552
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4592..4639
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4658..4702
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4763..4774
                     /note="Geneious type: Compositional bias"
                     /Note="Poly-Glu"
                     /standard_name="Compositional bias"
     misc_feature    4775..4855
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_feature    4805..5014
                     /note="Geneious type: domain"
                     /standard_name="RNA  (Arg/Ser rich)"
     misc_feature    5015..>5020
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    5017..5020
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    5017..2155
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of pOCC121 (concatenated
                     sequence 1)"
     misc_feature    5024..5047
                     /standard_name="PreScission"
     misc_feature    5057..5074
                     /standard_name="6xHis"
     misc_feature    5121..5151
                     /standard_name="POEM1-R primer"
     polyA_signal    complement(5155..5346)
     polyA_site      5247..5281
     misc_feature    complement(5294..5316)
                     /standard_name="AP00104-pOM1-seq.R"
     primer_bind     complement(6107..6123)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 rev primer bind"
     rep_origin      complement(6239..6921)
     rep_origin      complement(6257..6845)
     CDS             complement(7016..7876)
                     /standard_name="AmpR"
     misc_feature    7016..7043
                     /standard_name="AP00120-Amp-C-R"
     misc_feature    complement(7019..7037)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(7857..7876)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    7857..7876
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(7912..7947)
     misc_feature    complement(8214..8232)
                     /standard_name="subcloning(PhiC)-R"
     misc_feature    8223..8241
                     /standard_name="subcloning(PhiC)-R"
     misc_feature    8275..8292
                     /standard_name="AP00111-M13(AP_design)"
     misc_feature    complement(8283..8301)
                     /standard_name="AP0261-sgRNA_seq-F"
     primer_bind     8297..8314
                     /standard_name="M13-fwd"
     misc_feature    8297..8314
                     /standard_name="AP00110-M13(18mer)"
     primer_bind     8298..8314
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 fwd primer bind"
     primer_bind     8324..8344
                     /standard_name="T7"
     misc_feature    8325..8344
                     /standard_name="AP00109-T7-promoter"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggcatgaaaa tcgaagaagg
      241 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      301 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      361 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      421 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      481 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      541 ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc tgccgaaccc
      601 gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga aaggtaagag
      661 cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg
      721 gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa
      781 cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa
      841 tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac
      901 catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac
      961 ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg
     1021 tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct
     1081 gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa
     1141 gtcttacgag gaagagttgg tgaaagatcc gcgtattgcc gccactatgg aaaacgccca
     1201 gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac
     1261 tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca
     1321 gactaattcg agctcgaaca acaacaacaa taacaataac aacaacagca gcggcagact
     1381 ggaagttctg ttccaggggc cgatggtgag caagggcgag gagctgttca ccggggtggt
     1441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1561 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     1621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1801 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1861 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     1921 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1981 cgtgctgctg cccgacaacc actacctgag cacccagtcc aaactgagca aagaccccaa
     2041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     2101 catggacgag ctgtacaagg gatccgctgg ctccgctgct ggttctggcg cggccgcaat
     2161 gactgacgaa acagctcacc cgacacagag cgcctcgaaa caggagagtg cagctttgaa
     2221 gcagaccggc gatgaccagc aggagtccca gcagcagaga ggttatacaa actataacaa
     2281 cggaagcaac tacacccaga aaaagccata taacagtaac agaccacacc agcagagagg
     2341 aggtaaattc ggacccaacc gctataacaa cagaggaaat tataacggag gaggctcatt
     2401 tagaggaggc catatgggtg cgaactcatc aaatgtgcct tggaccggat attacaataa
     2461 ttatcctgta tactatcagc cgcaacagat ggccgcagca ggaagcgctc ctgcgaaccc
     2521 tattcctgta gaagaaaaga gtcccgtccc cacgaaaatt gaaataacaa ccaagtcagg
     2581 cgagcacctt gatctgaaag aacagcacaa ggcaaaactt caatcccaag agaggagcac
     2641 tgtttctccc caaccagaat ccaaacttaa agaaacgagc gatagtacat ctaccagtac
     2701 gccgactccc actccgtcca cgaatgatag caaagctagc agcgaagaaa atatctccga
     2761 ggcagaaaaa actcgtagga atttcatcga gcaggtaaag ctccgcaagg cggcgcttga
     2821 aaaaaagagg aaagagcaat tggagggaag ttcgggtaac aacaacatcc cgatgaagac
     2881 cactccagaa aacgtagaag aaaaaggatc agataaaccc gaggtcactg aaaaaaccaa
     2941 gcctgcagag gagaagtccg cggaacctga ggtcaaacaa gaaactcccg cagaggaagg
     3001 cgaacaaggc gagaaaggtc agattaaaga agagagtacg cctaaagttc tgactttcgc
     3061 ggaacgtctt aagctgaaga agcagcagaa ggaaagagag gaaaagacag agggtaagga
     3121 gaataaggaa gtgcctgttc aagaagagac aaagtccgcc atagaaagtg cgcctgtgcc
     3181 accttcagaa caagtaaaag aagagacgga ggttgctgag acggaacaat ccaatatcga
     3241 tgagtcagcc actaccccgg ctattccgac aaaaagcgat gaagctgagg cagaggtgga
     3301 agctgaagca ggagacgcgg gtacaaaaat cggactggaa gccgagattg agaccacaac
     3361 cgacgagact gacgatggca ccaacacagt cagtcatatt ttgaatgttc ttaaagacgc
     3421 tacgccaatt gaagatgtct tctcattcaa ttacccagaa ggtatcgaag gtccagacat
     3481 aaaatataaa aaggagcatg taaagtatac atatggccca actttcttgt tgcaattcaa
     3541 ggacaaactt aacgtcaagg ctgacgcaga atgggtccaa agtactgcta gtaaaatcgt
     3601 aattccccca ggcatgggcc gtggtaatcg cagccgcgac agtggacgtt ttggaaacaa
     3661 ctcaagccgc ggccacgact ttaggaacac ttcggtacgc aacatggatg accgcgctaa
     3721 ttcccgtacg agcagcaagc gtaggtcgaa gcgcatgaat gacgaccgca ggagcaatag
     3781 atcatacacc agccgcagag atagggaaag gggttcttat cgcaacgaag agaagcgtga
     3841 agacgacaag ccaaaggaag aagtcgctcc ccttgtaccg tcagctaaca ggtgggtacc
     3901 gaagttcaaa tcgaagaaaa ctgagaaaaa acttgcccct gatggcaaaa cggaactgct
     3961 cgataaagat gaagtggagc gcaagatgaa gtcacttttg aacaagttga ctctggagat
     4021 gtttgatgcc attagttctg aaatcctggc aatcgccaac atcagcgtat gggagacgaa
     4081 cggcgagacg cttaaggcgg taatagaaca aatctttctc aaagcgtgtg acgagccgca
     4141 ttggagctcc atgtatgctc agctgtgcgg caaagtagtg aaagaactca atccagacat
     4201 aacagatgaa accaacgagg gaaagactgg tccgaagttg gtcttgcact acctggtagc
     4261 tcgctgtcat gctgaattcg ataaaggttg gactgataag cttccgacga atgaagacgg
     4321 aaccccgctt gaacccgaga tgatgtctga ggagtactat gctgccgcgt ccgccaagag
     4381 gcgcggtctg ggacttgtcc gctttattgg cttcctctat cgtcttaacc ttcttacagg
     4441 caaaatgatg tttgaatgtt ttaggagact gatgaaggac ctcactgata gtcctagtga
     4501 ggagacgctg gaatccgttg ttgagctcct gaataccgta ggagagcagt tcgagactga
     4561 ctctttcagg accggacagg cgactcttga aggctcgcag ttgctcgact cgttgtttgg
     4621 aatcttggat aatattatac aaacagccaa gataagcagt agaataaaat ttaaacttat
     4681 agatattaag gagctcaggc acgacaaaaa ttggaatagt gacaaaaaag ataacggacc
     4741 gaaaactata caacagattc acgaggaaga agagaggcaa aggcagctta agaacaactc
     4801 taggagcaac tcaagacgca caaacaattc gtccaaccgt cacagcttcc gcagagacgc
     4861 tccgccagcc tcgaaggatt cgtttataac cacaagaact tatagccagc gcaacagcca
     4921 aagagcgcca ccgccaaagg aagaaccagc tgccccaaca agcacagcta cgaacatgtt
     4981 ctctgcgctc atgggtgagt ccgatgatga agaaggcgcg ccgctggaag ttctgttcca
     5041 ggggccgggc agcagccatc accatcacca tcacagtgga taatagaagc ttgtcgagaa
     5101 gtactagagg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     5161 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     5221 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5281 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5341 tgtctggatc tgatcactgc ttgagcctag gagatccgaa ccagataagt gaaagcctgc
     5401 atccgcagat cttaattaat aaaacacgat acattgttat tagtacattt attaagcgct
     5461 agattctgtg cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa
     5521 tttataatct ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata
     5581 tctgaattta aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa
     5641 caagggttgt ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac
     5701 aaaacttgcc aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt
     5761 ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact
     5821 gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat aacgcttgga catatttaac
     5881 atcgggcgtg ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga
     5941 agttgcttcc gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa
     6001 cacgtccgcg atcaaatttg tagttgagct ttttgggcct catgggcctt ccgctcactg
     6061 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aacatggtca tagctgtttc
     6121 cttgcgtatt gggcgctctc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6181 gtaaagcctg gggtgcctaa tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6241 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6301 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6361 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6421 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6481 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6541 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6601 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6661 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6721 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6781 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6841 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6901 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6961 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7021 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7081 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7141 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7201 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7261 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7321 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7381 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7441 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7501 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7561 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7621 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7681 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7741 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7801 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7861 aatgttgaat actcattact cttccttttt caatattatt gaagcattta tcagggttat
     7921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7981 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     8041 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     8101 cttataaatc aaaagaatag accgagatag ggttgagtgg ccgctacagg gcgctcccat
     8161 tcgccattca ggctgcgcaa ctgttgggaa gggcgtttcg gtgcgggcct cttcgctatt
     8221 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8281 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gacgtaatac gactcactat
     8341 agggcgaatt ggcggaaggc cgtcaaggcc gtgacgttaa aactattaag ccatccaatc
     8401 gaccgttagt cgaatcagga ccgctggtgc gagaagccgc gaagtatggc gaatgcatcg
     8461 tataacgtgt ggagtccgct cattagagcg tcatgtttag acaagaaagc tacatattta
     8521 attgatcccg atgattttat tgataaattg accctaactc catacacggt attctacaat
     8581 ggcggggttt tggtcaaaat ttccggactg cgattgtaca tgctgttaac ggctccgccc
     8641 actattaatg aaattaaaaa ttccaatttt aaaaaacgca gcaagagaaa catttgtatg
     8701 aaagaatgcg tagaaggaaa gaaaaatgtc gtggacatgc tgaacaacaa gattaatatg
     8761 cctccgtgta taaaaaaaat attgaacgat ttgaaagaaa acaatgtacc gcgcggcggt
     8821 atgtacagga agaggtttat actaaactgt tacattgcaa acgtggtttc gtgtgccaag
     8881 tgtgaaaacc gatgtttaat caaggctctg acgcatttct acaaccacga ctccaagtgt
     8941 gtgggtgaag tcatgcatct tttaatcaaa tcccaagatg tgtataaacc accaaactgc
     9001 caaaaaatga aaactgtgga caagctctgt ccgtttgctg gcaactgcaa gggtctcaat
     9061 cctatttgta attattgaat aataaaacaa ttataaatgc taaatttgtt ttttattaac
     9121 gatacaaacc aaacgcaaca agaacatttg tagtattatc tataattgaa aacgcgtagt
     9181 tataatcgct gaggtaatat ttaaaatcat tttcaaatga ttcacagtta atttgcgaca
     9241 atataatttt attttcacat aaactagacg ccttgtcgtc ttcttcttcg tattccttct
     9301 ctttttcatt tttctcctca taaaaattaa catagttatt atcgtatcca tatatgtatc
     9361 tatcgtatag agtaaatttt ttgttgtcat aaatatatat gtctttttta atggggtgta
     9421 tagtaccgct gcgcatagtt tttctgtaat ttacaacagt gctattttct ggtagttctt
     9481 cggagtgtgt tgctttaatt attaaattta tataatcaat gaatttggga tcgtcggttt
     9541 tgtacaatat gttgccggca tagtacgcag cttcttctag ttcaattaca ccatttttta
     9601 gcagcaccgg attaacataa ctttccaaaa tgttgtacga accgttaaac aaaaacagtt
     9661 cacctccctt ttctatacta ttgtctgcga gcagttgttt gttgttaaaa ataacagcca
     9721 ttgtaatgag acgcacaaac taatatcaca
//
