LOCUS       pOCC102-MBP-eIF4        7512 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...1j-hkp6539
VERSION     urn.local...1j-hkp6539
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC102 (digested by AscI, NotI) Fragment
                     2 - NotI/AscI Digestion of pOCC102 (concatenated sequence
                     1) (concatenated sequence 1)"
     misc_feature    38..57
                     /standard_name="AP00102-pOM1-seq.F1"
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    126..151
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pOCC295-fw"
     misc_feature    174..201
                     /standard_name="POEM1-F primer"
     misc_feature    174..196
                     /standard_name="AP00103-pOM1-seq.F2"
     misc_feature    224..241
                     /standard_name="6xHis"
     exon            254..1396
                     /standard_name="MBP from pMAL-c5G"
     misc_feature    complement(316..338)
                     /standard_name="AP0267-MBP-nR"
     misc_feature    1319..1339
                     /standard_name="AP0268-MBP-cF"
     misc_feature    1409..1432
                     /standard_name="PreScission"
     misc_feature    join(1433..>1438,<2836..2841)
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    1435..2839
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="R2-4ABD-R3 (digested by AscI, NotI)
                     Fragment 2 (concatenated sequence 2)"
     misc_feature    <1435..1444
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <1435..>1440
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    1435..1438
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    join(1435..>1438,<2836..2839)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of NotI/AscI Tif4631 optimized
                     (IDT) no STOP - Fragment 2 (concatenated sequence 2)"
     misc_feature    <1435..>1438
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    <1445..>2839
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_RNA        1445..1603
                     /standard_name="RNA2"
     misc_feature    <1445..1447
                     /note="Geneious type: region"
                     /standard_name="eIF4E binding "
     misc_feature    <1445..1636
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_feature    1445..1603
                     /note="Geneious type: domain"
                     /standard_name="RNA (Arg/Ser rich)"
     misc_feature    1601..2626
                     /note="Geneious type: region"
                     /standard_name="Tif1/2 (eIF4A) binding"
     misc_feature    1737..1748
                     /note="Geneious type: Mutagenesis"
                     /Note="In TIF4631-213; abolishes interaction with PAB1 and
                     inhibits poly(A)-dependent translation. KLRK->AAAA"
                     /standard_name="Mutagenesis"
     misc_feature    1784..1822
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    1796..2527
                     /note="Geneious type: domain"
                     /Note="MIF4G"
                     /standard_name="MIF4G (rich in alpha-helices)"
     misc_feature    1841..1879
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    1880..1891
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    1904..1951
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    1964..2005
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2051..2104
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2174..2242
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     primer_bind     2213..2228
                     /standard_name="4G_pUC57_2000_fw"
     primer_bind     complement(2213..2228)
                     /standard_name="4G_pUC57_2000_rv"
     misc_feature    2258..2305
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2318..2371
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2411..2458
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2477..2521
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2582..2593
                     /note="Geneious type: Compositional bias"
                     /Note="Poly-Glu"
                     /standard_name="Compositional bias"
     misc_feature    2594..2674
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_RNA        2624..2833
                     /standard_name="RNA3"
     misc_feature    2624..2833
                     /note="Geneious type: domain"
                     /standard_name="RNA  (Arg/Ser rich)"
     misc_feature    2834..>2839
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    2836..2839
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    2836..7512
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC102 (digested by AscI, NotI) Fragment
                     2 - NotI/AscI Digestion of pOCC102 (concatenated sequence
                     1) (concatenated sequence 3)"
     misc_feature    <2836..>2839
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    2836..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    2883..2913
                     /standard_name="POEM1-R primer"
     polyA_signal    complement(2917..3108)
     polyA_site      3009..3043
     misc_feature    complement(3056..3078)
                     /standard_name="AP00104-pOM1-seq.R"
     primer_bind     complement(3869..3885)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 rev primer bind"
     rep_origin      complement(4001..4683)
     rep_origin      complement(4019..4607)
     CDS             complement(4778..5638)
                     /standard_name="AmpR"
     misc_feature    4778..4805
                     /standard_name="AP00120-Amp-C-R"
     misc_feature    complement(4781..4799)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(5619..5638)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    5619..5638
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(5674..5709)
     misc_feature    complement(5976..5994)
                     /standard_name="subcloning(PhiC)-R"
     misc_feature    6037..6054
                     /standard_name="AP00111-M13(AP_design)"
     misc_feature    complement(6045..6063)
                     /standard_name="AP0261-sgRNA_seq-F"
     primer_bind     6059..6076
                     /standard_name="M13-fwd"
     misc_feature    6059..6076
                     /standard_name="AP00110-M13(18mer)"
     primer_bind     6060..6076
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 fwd primer bind"
     primer_bind     6086..6106
                     /standard_name="T7"
     misc_feature    6087..6106
                     /standard_name="AP00109-T7-promoter"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggccatcacc atcaccatca
      241 cagcagcggc agaatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      301 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      361 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      421 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      481 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      541 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      601 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      661 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      721 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      781 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      841 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      901 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
      961 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     1021 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     1081 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     1141 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg tgaaagatcc
     1201 gcgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     1261 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     1321 gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca acaacaacaa
     1381 taacaataac aacaacagca gcggcagact ggaagttctg ttccaggggc cggcggccgc
     1441 aatgaatcgc agccgcgaca gtggacgttt tggaaacaac tcaagccgcg gccacgactt
     1501 taggaacact tcggtacgca acatggatga ccgcgctaat tcccgtacga gcagcaagcg
     1561 taggtcgaag cgcatgaatg acgaccgcag gagcaataga tcatacacca gccgcagaga
     1621 tagggaaagg ggttcttatc gcaacgaaga gaagcgtgaa gacgacaagc caaaggaaga
     1681 agtcgctccc cttgtaccgt cagctaacag gtgggtaccg aagttcaaat cgaagaaaac
     1741 tgagaaaaaa cttgcccctg atggcaaaac ggaactgctc gataaagatg aagtggagcg
     1801 caagatgaag tcacttttga acaagttgac tctggagatg tttgatgcca ttagttctga
     1861 aatcctggca atcgccaaca tcagcgtatg ggagacgaac ggcgagacgc ttaaggcggt
     1921 aatagaacaa atctttctca aagcgtgtga cgagccgcat tggagctcca tgtatgctca
     1981 gctgtgcggc aaagtagtga aagaactcaa tccagacata acagatgaaa ccaacgaggg
     2041 aaagactggt ccgaagttgg tcttgcacta cctggtagct cgctgtcatg ctgaattcga
     2101 taaaggttgg actgataagc ttccgacgaa tgaagacgga accccgcttg aacccgagat
     2161 gatgtctgag gagtactatg ctgccgcgtc cgccaagagg cgcggtctgg gacttgtccg
     2221 ctttattggc ttcctctatc gtcttaacct tcttacaggc aaaatgatgt ttgaatgttt
     2281 taggagactg atgaaggacc tcactgatag tcctagtgag gagacgctgg aatccgttgt
     2341 tgagctcctg aataccgtag gagagcagtt cgagactgac tctttcagga ccggacaggc
     2401 gactcttgaa ggctcgcagt tgctcgactc gttgtttgga atcttggata atattataca
     2461 aacagccaag ataagcagta gaataaaatt taaacttata gatattaagg agctcaggca
     2521 cgacaaaaat tggaatagtg acaaaaaaga taacggaccg aaaactatac aacagattca
     2581 cgaggaagaa gagaggcaaa ggcagcttaa gaacaactct aggagcaact caagacgcac
     2641 aaacaattcg tccaaccgtc acagcttccg cagagacgct ccgccagcct cgaaggattc
     2701 gtttataacc acaagaactt atagccagcg caacagccaa agagcgccac cgccaaagga
     2761 agaaccagct gccccaacaa gcacagctac gaacatgttc tctgcgctca tgggtgagtc
     2821 cgatgatgaa gaaggcgcgc cgtaatagaa gcttgtcgag aagtactaga ggatcataat
     2881 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2941 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     3001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     3061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact
     3121 gcttgagcct aggagatccg aaccagataa gtgaaagcct gcatccgcag atcttaatta
     3181 ataaaacacg atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg
     3241 atttacagac aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg
     3301 gtatgttaga gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa
     3361 atcctcaata gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga
     3421 accgatggct ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg
     3481 tagcagcaat ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac
     3541 gtcgttcaaa atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg
     3601 actcgacgta aacacgttaa ataacgcttg gacatattta acatcgggcg tgttagcttt
     3661 attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga
     3721 ttttgccata gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt
     3781 tgtagttgag ctttttgggc ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga
     3841 aacctgtcgt gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc
     3901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct
     3961 aatgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     5101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     5401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatta
     5641 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5701 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5761 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     5821 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     5881 agaccgagat agggttgagt ggccgctaca gggcgctccc attcgccatt caggctgcgc
     5941 aactgttggg aagggcgttt cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     6001 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     6061 taaaacgacg gccagtgagc gcgacgtaat acgactcact atagggcgaa ttggcggaag
     6121 gccgtcaagg ccgtgacgtt aaaactatta agccatccaa tcgaccgtta gtcgaatcag
     6181 gaccgctggt gcgagaagcc gcgaagtatg gcgaatgcat cgtataacgt gtggagtccg
     6241 ctcattagag cgtcatgttt agacaagaaa gctacatatt taattgatcc cgatgatttt
     6301 attgataaat tgaccctaac tccatacacg gtattctaca atggcggggt tttggtcaaa
     6361 atttccggac tgcgattgta catgctgtta acggctccgc ccactattaa tgaaattaaa
     6421 aattccaatt ttaaaaaacg cagcaagaga aacatttgta tgaaagaatg cgtagaagga
     6481 aagaaaaatg tcgtggacat gctgaacaac aagattaata tgcctccgtg tataaaaaaa
     6541 atattgaacg atttgaaaga aaacaatgta ccgcgcggcg gtatgtacag gaagaggttt
     6601 atactaaact gttacattgc aaacgtggtt tcgtgtgcca agtgtgaaaa ccgatgttta
     6661 atcaaggctc tgacgcattt ctacaaccac gactccaagt gtgtgggtga agtcatgcat
     6721 cttttaatca aatcccaaga tgtgtataaa ccaccaaact gccaaaaaat gaaaactgtg
     6781 gacaagctct gtccgtttgc tggcaactgc aagggtctca atcctatttg taattattga
     6841 ataataaaac aattataaat gctaaatttg ttttttatta acgatacaaa ccaaacgcaa
     6901 caagaacatt tgtagtatta tctataattg aaaacgcgta gttataatcg ctgaggtaat
     6961 atttaaaatc attttcaaat gattcacagt taatttgcga caatataatt ttattttcac
     7021 ataaactaga cgccttgtcg tcttcttctt cgtattcctt ctctttttca tttttctcct
     7081 cataaaaatt aacatagtta ttatcgtatc catatatgta tctatcgtat agagtaaatt
     7141 ttttgttgtc ataaatatat atgtcttttt taatggggtg tatagtaccg ctgcgcatag
     7201 tttttctgta atttacaaca gtgctatttt ctggtagttc ttcggagtgt gttgctttaa
     7261 ttattaaatt tatataatca atgaatttgg gatcgtcggt tttgtacaat atgttgccgg
     7321 catagtacgc agcttcttct agttcaatta caccattttt tagcagcacc ggattaacat
     7381 aactttccaa aatgttgtac gaaccgttaa acaaaaacag ttcacctccc ttttctatac
     7441 tattgtctgc gagcagttgt ttgttgttaa aaataacagc cattgtaatg agacgcacaa
     7501 actaatatca ca
//
