LOCUS       pOCC102-MBP-eIF4        8976 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...t-hkp60il
VERSION     urn.local...t-hkp60il
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    38..57
                     /standard_name="AP00102-pOM1-seq.F1"
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    126..151
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name="pOCC295-fw"
     misc_feature    174..201
                     /standard_name="POEM1-F primer"
     misc_feature    174..196
                     /standard_name="AP00103-pOM1-seq.F2"
     misc_feature    224..241
                     /standard_name="6xHis"
     exon            254..1396
                     /standard_name="MBP from pMAL-c5G"
     misc_feature    complement(316..338)
                     /standard_name="AP0267-MBP-nR"
     misc_feature    1319..1339
                     /standard_name="AP0268-MBP-cF"
     misc_feature    1409..1432
                     /standard_name="PreScission"
     misc_feature    1433..4305
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    <1435..1441
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1435..4303
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="Digestion of NotI/AscI Tif4631 optimized
                     (IDT) no STOP - Fragment 2 (concatenated sequence 2)"
     misc_feature    1435..1438
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1442..1687
                     /note="Geneious type: domain"
                     /standard_name="RNA"
     misc_feature    1724..1774
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box1 "
     misc_feature    1823..1891
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box2"
     misc_feature    1958..2041
                     /note="Geneious type: Compositional bias"
                     /Note="Pro/Ser/Thr-rich"
                     /standard_name="Compositional bias"
     misc_feature    2003..2338
                     /note="Geneious type: region"
                     /Note="Interaction with PAB1"
                     /standard_name="Interaction with Pab1"
     misc_feature    2033..2113
                     /note="Geneious type: Compositional bias"
                     /standard_name="Box3"
     misc_feature    2078..2089
                     /note="Geneious type: site"
                     /standard_name="ts interaction with Pab1"
     misc_feature    2081..2092
                     /note="Geneious type: Mutagenesis"
                     /Note="In TIF4631-213; abolishes interaction with PAB1 and
                     inhibits poly(A)-dependent translation. KLRK->AAAA"
                     /standard_name="Mutagenesis"
     misc_feature    2564..2593
                     /note="Geneious type: Compositional bias"
                     /Note="Ala/Glu-rich"
                     /standard_name="Compositional bias"
     misc_feature    2612..2911
                     /note="Geneious type: region"
                     /standard_name="eIF4E binding "
     misc_feature    2672..2698
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     primer_bind     complement(2783..2809)
                     /standard_name="pOCC_4G_459_rv"
     misc_feature    2801..2833
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2810..2811
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TT"
     primer_bind     2810..2825
                     /standard_name="pOCC_4G_459_fw"
     misc_feature    <2812..2815
                     /note="Geneious type: site"
                     /standard_name="ts interaction with eIF4E"
     misc_feature    2813..2814
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TT"
     misc_feature    2852..2869
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    2903..3100
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_feature    2909..3067
                     /note="Geneious type: domain"
                     /standard_name="RNA (Arg/Ser rich)"
     misc_feature    3065..4090
                     /note="Geneious type: region"
                     /standard_name="Tif1/2 (eIF4A) binding"
     misc_feature    3201..3212
                     /note="Geneious type: Mutagenesis"
                     /Note="In TIF4631-213; abolishes interaction with PAB1 and
                     inhibits poly(A)-dependent translation. KLRK->AAAA"
                     /standard_name="Mutagenesis"
     misc_feature    3248..3286
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3260..3991
                     /note="Geneious type: domain"
                     /Note="MIF4G"
                     /standard_name="MIF4G (rich in alpha-helices)"
     misc_feature    3305..3343
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3344..3355
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3368..3415
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3428..3469
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3515..3568
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3638..3706
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3722..3769
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3782..3835
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3875..3922
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    3941..3985
                     /note="Geneious type: Helix"
                     /standard_name="Helix"
     misc_feature    4046..4057
                     /note="Geneious type: Compositional bias"
                     /Note="Poly-Glu"
                     /standard_name="Compositional bias"
     misc_feature    4058..4138
                     /note="Geneious type: Compositional bias"
                     /Note="Arg/Ser-rich"
                     /standard_name="Compositional bias"
     misc_feature    4088..4297
                     /note="Geneious type: domain"
                     /standard_name="RNA  (Arg/Ser rich)"
     misc_feature    4298..>4303
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    4300..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    4300..4303
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    4347..4377
                     /standard_name="POEM1-R primer"
     polyA_signal    complement(4381..4572)
     polyA_site      4473..4507
     misc_feature    complement(4520..4542)
                     /standard_name="AP00104-pOM1-seq.R"
     primer_bind     complement(5333..5349)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 rev primer bind"
     rep_origin      complement(5465..6147)
     rep_origin      complement(5483..6071)
     CDS             complement(6242..7102)
                     /standard_name="AmpR"
     misc_feature    6242..6269
                     /standard_name="AP00120-Amp-C-R"
     misc_feature    complement(6245..6263)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(7083..7102)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    7083..7102
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(7138..7173)
     misc_feature    complement(7440..7458)
                     /standard_name="subcloning(PhiC)-R"
     misc_feature    7501..7518
                     /standard_name="AP00111-M13(AP_design)"
     misc_feature    complement(7509..7527)
                     /standard_name="AP0261-sgRNA_seq-F"
     primer_bind     7523..7540
                     /standard_name="M13-fwd"
     misc_feature    7523..7540
                     /standard_name="AP00110-M13(18mer)"
     primer_bind     7524..7540
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /standard_name="M13 fwd primer bind"
     primer_bind     7550..7570
                     /standard_name="T7"
     misc_feature    7551..7570
                     /standard_name="AP00109-T7-promoter"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggccatcacc atcaccatca
      241 cagcagcggc agaatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      301 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      361 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      421 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      481 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      541 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      601 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      661 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      721 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      781 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      841 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      901 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
      961 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     1021 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     1081 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     1141 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg tgaaagatcc
     1201 gcgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     1261 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     1321 gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca acaacaacaa
     1381 taacaataac aacaacagca gcggcagact ggaagttctg ttccaggggc cggcggccgc
     1441 aatgactgac gaaacagctc acccgacaca gagcgcctcg aaacaggaga gtgcagcttt
     1501 gaagcagacc ggcgatgacc agcaggagtc ccagcagcag agaggttata caaactataa
     1561 caacggaagc aactacaccc agaaaaagcc atataacagt aacagaccac accagcagag
     1621 aggaggtaaa ttcggaccca accgctataa caacagagga aattataacg gaggaggctc
     1681 atttagagga ggccatatgg gtgcgaactc atcaaatgtg ccttggaccg gatattacaa
     1741 taattatcct gtatactatc agccgcaaca gatggccgca gcaggaagcg ctcctgcgaa
     1801 ccctattcct gtagaagaaa agagtcccgt ccccacgaaa attgaaataa caaccaagtc
     1861 aggcgagcac cttgatctga aagaacagca caaggcaaaa cttcaatccc aagagaggag
     1921 cactgtttct ccccaaccag aatccaaact taaagaaacg agcgatagta catctaccag
     1981 tacgccgact cccactccgt ccacgaatga tagcaaagct agcagcgaag aaaatatctc
     2041 cgaggcagaa aaaactcgta ggaatttcat cgagcaggta aagctccgca aggcggcgct
     2101 tgaaaaaaag aggaaagagc aattggaggg aagttcgggt aacaacaaca tcccgatgaa
     2161 gaccactcca gaaaacgtag aagaaaaagg atcagataaa cccgaggtca ctgaaaaaac
     2221 caagcctgca gaggagaagt ccgcggaacc tgaggtcaaa caagaaactc ccgcagagga
     2281 aggcgaacaa ggcgagaaag gtcagattaa agaagagagt acgcctaaag ttctgacttt
     2341 cgcggaacgt cttaagctga agaagcagca gaaggaaaga gaggaaaaga cagagggtaa
     2401 ggagaataag gaagtgcctg ttcaagaaga gacaaagtcc gccatagaaa gtgcgcctgt
     2461 gccaccttca gaacaagtaa aagaagagac ggaggttgct gagacggaac aatccaatat
     2521 cgatgagtca gccactaccc cggctattcc gacaaaaagc gatgaagctg aggcagaggt
     2581 ggaagctgaa gcaggagacg cgggtacaaa aatcggactg gaagccgaga ttgagaccac
     2641 aaccgacgag actgacgatg gcaccaacac agtcagtcat attttgaatg ttcttaaaga
     2701 cgctacgcca attgaagatg tcttctcatt caattaccca gaaggtatcg aaggtccaga
     2761 cataaaatat aaaaaggagc atgtaaagta tacatatggc ccaactttcg cggcgcaatt
     2821 caaggacaaa cttaacgtca aggctgacgc agaatgggtc caaagtactg ctagtaaaat
     2881 cgtaattccc ccaggcatgg gccgtggtaa tcgcagccgc gacagtggac gttttggaaa
     2941 caactcaagc cgcggccacg actttaggaa cacttcggta cgcaacatgg atgaccgcgc
     3001 taattcccgt acgagcagca agcgtaggtc gaagcgcatg aatgacgacc gcaggagcaa
     3061 tagatcatac accagccgca gagataggga aaggggttct tatcgcaacg aagagaagcg
     3121 tgaagacgac aagccaaagg aagaagtcgc tccccttgta ccgtcagcta acaggtgggt
     3181 accgaagttc aaatcgaaga aaactgagaa aaaacttgcc cctgatggca aaacggaact
     3241 gctcgataaa gatgaagtgg agcgcaagat gaagtcactt ttgaacaagt tgactctgga
     3301 gatgtttgat gccattagtt ctgaaatcct ggcaatcgcc aacatcagcg tatgggagac
     3361 gaacggcgag acgcttaagg cggtaataga acaaatcttt ctcaaagcgt gtgacgagcc
     3421 gcattggagc tccatgtatg ctcagctgtg cggcaaagta gtgaaagaac tcaatccaga
     3481 cataacagat gaaaccaacg agggaaagac tggtccgaag ttggtcttgc actacctggt
     3541 agctcgctgt catgctgaat tcgataaagg ttggactgat aagcttccga cgaatgaaga
     3601 cggaaccccg cttgaacccg agatgatgtc tgaggagtac tatgctgccg cgtccgccaa
     3661 gaggcgcggt ctgggacttg tccgctttat tggcttcctc tatcgtctta accttcttac
     3721 aggcaaaatg atgtttgaat gttttaggag actgatgaag gacctcactg atagtcctag
     3781 tgaggagacg ctggaatccg ttgttgagct cctgaatacc gtaggagagc agttcgagac
     3841 tgactctttc aggaccggac aggcgactct tgaaggctcg cagttgctcg actcgttgtt
     3901 tggaatcttg gataatatta tacaaacagc caagataagc agtagaataa aatttaaact
     3961 tatagatatt aaggagctca ggcacgacaa aaattggaat agtgacaaaa aagataacgg
     4021 accgaaaact atacaacaga ttcacgagga agaagagagg caaaggcagc ttaagaacaa
     4081 ctctaggagc aactcaagac gcacaaacaa ttcgtccaac cgtcacagct tccgcagaga
     4141 cgctccgcca gcctcgaagg attcgtttat aaccacaaga acttatagcc agcgcaacag
     4201 ccaaagagcg ccaccgccaa aggaagaacc agctgcccca acaagcacag ctacgaacat
     4261 gttctctgcg ctcatgggtg agtccgatga tgaagaaggc gcgccgtaat agaagcttgt
     4321 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4381 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4441 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4501 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4561 ttatcatgtc tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa
     4621 gcctgcatcc gcagatctta attaataaaa cacgatacat tgttattagt acatttatta
     4681 agcgctagat tctgtgcgtt gttgatttac agacaattgt tgtacgtatt ttaataattc
     4741 attaaattta taatctttag ggtggtatgt tagagcgaaa atcaaatgat tttcagcgtc
     4801 tttatatctg aatttaaata ttaaatcctc aatagatttg taaaataggt ttcgattagt
     4861 ttcaaacaag ggttgttttt ccgaaccgat ggctggacta tctaatggat tttcgctcaa
     4921 cgccacaaaa cttgccaaat cttgtagcag caatctagct ttgtcgatat tcgtttgtgt
     4981 tttgttttgt aataaaggtt cgacgtcgtt caaaatatta tgcgcttttg tatttctttc
     5041 atcactgtcg ttagtgtaca attgactcga cgtaaacacg ttaaataacg cttggacata
     5101 tttaacatcg ggcgtgttag ctttattagg ccgattatcg tcgtcgtccc aaccctcgtc
     5161 gttagaagtt gcttccgaag acgattttgc catagccaca cgacgcctat taattgtgtc
     5221 ggctaacacg tccgcgatca aatttgtagt tgagcttttt gggcctcatg ggccttccgc
     5281 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc
     5341 tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc
     5401 gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta
     5461 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5521 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5581 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5641 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5701 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5761 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5821 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5881 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5941 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6001 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6061 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6121 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6181 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6241 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6301 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6361 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6421 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6481 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6541 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6601 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6661 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6721 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6781 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6841 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6901 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6961 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7021 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7081 cacggaaatg ttgaatactc attactcttc ctttttcaat attattgaag catttatcag
     7141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7201 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     7261 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     7321 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtggccgc tacagggcgc
     7381 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gtttcggtgc gggcctcttc
     7441 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     7501 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgacg taatacgact
     7561 cactataggg cgaattggcg gaaggccgtc aaggccgtga cgttaaaact attaagccat
     7621 ccaatcgacc gttagtcgaa tcaggaccgc tggtgcgaga agccgcgaag tatggcgaat
     7681 gcatcgtata acgtgtggag tccgctcatt agagcgtcat gtttagacaa gaaagctaca
     7741 tatttaattg atcccgatga ttttattgat aaattgaccc taactccata cacggtattc
     7801 tacaatggcg gggttttggt caaaatttcc ggactgcgat tgtacatgct gttaacggct
     7861 ccgcccacta ttaatgaaat taaaaattcc aattttaaaa aacgcagcaa gagaaacatt
     7921 tgtatgaaag aatgcgtaga aggaaagaaa aatgtcgtgg acatgctgaa caacaagatt
     7981 aatatgcctc cgtgtataaa aaaaatattg aacgatttga aagaaaacaa tgtaccgcgc
     8041 ggcggtatgt acaggaagag gtttatacta aactgttaca ttgcaaacgt ggtttcgtgt
     8101 gccaagtgtg aaaaccgatg tttaatcaag gctctgacgc atttctacaa ccacgactcc
     8161 aagtgtgtgg gtgaagtcat gcatctttta atcaaatccc aagatgtgta taaaccacca
     8221 aactgccaaa aaatgaaaac tgtggacaag ctctgtccgt ttgctggcaa ctgcaagggt
     8281 ctcaatccta tttgtaatta ttgaataata aaacaattat aaatgctaaa tttgtttttt
     8341 attaacgata caaaccaaac gcaacaagaa catttgtagt attatctata attgaaaacg
     8401 cgtagttata atcgctgagg taatatttaa aatcattttc aaatgattca cagttaattt
     8461 gcgacaatat aattttattt tcacataaac tagacgcctt gtcgtcttct tcttcgtatt
     8521 ccttctcttt ttcatttttc tcctcataaa aattaacata gttattatcg tatccatata
     8581 tgtatctatc gtatagagta aattttttgt tgtcataaat atatatgtct tttttaatgg
     8641 ggtgtatagt accgctgcgc atagtttttc tgtaatttac aacagtgcta ttttctggta
     8701 gttcttcgga gtgtgttgct ttaattatta aatttatata atcaatgaat ttgggatcgt
     8761 cggttttgta caatatgttg ccggcatagt acgcagcttc ttctagttca attacaccat
     8821 tttttagcag caccggatta acataacttt ccaaaatgtt gtacgaaccg ttaaacaaaa
     8881 acagttcacc tcccttttct atactattgt ctgcgagcag ttgtttgttg ttaaaaataa
     8941 cagccattgt aatgagacgc acaaactaat atcaca
//
