LOCUS       pOCC102-MBP-eIF4        8973 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...25-hkp6g8u
VERSION     urn.local...25-hkp6g8u
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    224..241
                     /standard_name="6xHis"
     exon            254..1396
                     /standard_name="MBP from pMAL-c5G"
     primer_bind     1345..1362
                     /standard_name=1995
     misc_feature    1409..1432
                     /standard_name="PreScission"
     misc_feature    1435..4300
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI SK_eIF4G optomized IDT no STOP
                     (digested by AscI, NotI) (concatenated sequence 2)"
     misc_feature    <1435..1441
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1435..1438
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1442..4294
                     /note="Geneious type: ORF"
                     /standard_name="SK eIF4G"
     misc_feature    4295..>4300
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    4297..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pOCC102 (digested by NotI, AscI) Fragment
                     2 (concatenated sequence 1)"
     misc_feature    4297..4300
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     polyA_signal    complement(4378..4569)
     misc_feature    complement(4395..4412)
                     /note="Geneious type: primer_bind_reverse"
                     /standard_name=2306
     polyA_site      4470..4504
     rep_origin      complement(5462..6144)
     CDS             complement(6239..7099)
                     /standard_name="AmpR"
     misc_feature    complement(6242..6260)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(7080..7099)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    7080..7099
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(7135..7170)
     primer_bind     7520..7537
                     /standard_name="M13-fwd"
     primer_bind     7547..7567
                     /standard_name="T7"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggccatcacc atcaccatca
      241 cagcagcggc agaatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      301 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      361 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      421 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      481 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      541 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      601 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      661 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      721 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      781 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      841 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      901 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
      961 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     1021 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     1081 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     1141 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg tgaaagatcc
     1201 gcgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     1261 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     1321 gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca acaacaacaa
     1381 taacaataac aacaacagca gcggcagact ggaagttctg ttccaggggc cggcggccgc
     1441 aatgactgat gaaacagcac aacctaccca gagcgcaccc aggcaagaat ccgcagcccc
     1501 gaaacagacg gacgacgacc aacaggtttc acaacagcaa caacagaggg gttacaacaa
     1561 ttataatgac ggctcaaatt acaaccaaaa aaagccatat aatagtaaca gaccccatca
     1621 gcagcgtggc ggcaagtttg gcccaaacag atataattca agaggtaatt ataacggagg
     1681 tggaaactat aggggcggac acatgggagc taacggatcg aatgttccat ggacgggtta
     1741 ttacaataat tatcctgtct actaccaacc tcaacagatg ggtgcgggaa acgcaatccc
     1801 agttaatgta gcccccgcag atgaaaaatc ttctccggtg cctaccaaga ttgagattac
     1861 cacaaagtct ggagagcatc tcgatcttaa agagcaacat aaggtgaaat tgcagtctaa
     1921 agaaacctct actgcaccaa ctcccctgga tcccaagtcg gaagaagcca gcgactcggc
     1981 acctacgcct acgcctacac ctgcaaccac agactcgaaa gagtcttcag aagagaacat
     2041 atcagaggca gagaagacga gaaggaattt tattgaacag gtaaagcttc gtaaggctgc
     2101 gttggaaaag aagcgtaaag aacaattgga gggcgctggt ggttcgtcca acgtatcaac
     2161 tgagaccgct cctgtcaaag ccgaagaaac agagcccgtg atctctgaag cgggtgaaaa
     2221 aaagccggcc gagctggaga caaaccagga cacaccttcg gaagaaaacg gccaggagga
     2281 gcagggtcag ataaaagagg agagtacaac gaaggcgctg acattcgctg aaagacttaa
     2341 gctcaagaaa caacagaagg aacaggagaa gactgaagag aaagaaaatc aagaagagcc
     2401 tgtgcaagaa gaggcgaatt ccgtgactaa aacagcgacc gtgccgtcca cagagcaaga
     2461 tcaagaacag acagaaacaa gtgagacgga acgcagcgag gtagaagaaa gcacaccaca
     2521 aaccgccgag ccaacagcca caatcccggg aaccctcgcg gaatctggcg aggcggacga
     2581 cacggaagtc aaagtcgagt cagaggccga ggtagaggca gacatagaga cagcggctga
     2641 cgatgaagca gacaacggaa caaatacagt tagccacgtg ctgaatgtcc tgaaggacgc
     2701 caggcccata gaagatgtgt tttcatttat ctatccagaa ggtattgagg gtcctgacat
     2761 taaatataaa aaagaacacg tgaagtatac ttacggtcca acgttcctcc ttcagttcaa
     2821 ggacaaactg aacgtaaaag cagatgcaga gtgggtacag tcaacagcga gtaagattgt
     2881 catacccccg ggcatgggcc gtggaaataa gtctagggat ccgtcaagat ttagcaataa
     2941 ctcgtccagg ggtcacgact tcaggagcaa ctcagtacgc aatatggatg acagagcaaa
     3001 cagtagaacc tcttcgaagc gtcgttccaa acgtatgaac gacgataggc gctcaaaccg
     3061 cagctacaca agcagaagag atagggagag gggctcctac cgcaatgagg ataagcgcga
     3121 tgacgatagg ccccgtgaag aagtggcccc actcgttcca tctagcaatc gttggatacc
     3181 taaatccaaa gccaagtcca ccgaaaaaaa gcttgcgcct gacggaaaga ccgagctttt
     3241 ggacaaggat gaagtcgaga gaaagatgaa aagccttttg aacaagctga ctttggagat
     3301 gtttgatgct atcagttctg agattttggc tattgcgaac atctccgtct gggaaacgaa
     3361 cggcgagact ctgaaagcgg tgatagaaca agtatttctg aaagcgtgcg acgaacctca
     3421 ttggtcctca atgtacgcac aattgtgcgg aaaagtcgtt aaagagctta atcctgacat
     3481 aagcgatgaa acgaacgaag gcaagactgg tccgaaactt gtattgcatt atctggttgc
     3541 caggtgtcat gcggaatttg acaagggctg gacagacaag ctgccgacca aagaagacgg
     3601 cacgccgctc gagcctgaaa tgatgtccga agagtactat gcagctgcat cggcaaaaag
     3661 aaggggtctt ggcttggtta gatttatcgg attcctttac cgtttgaacc tcttgacggg
     3721 taaaatgatg ttcgaatgct ttagacgtct gatgaaagat ttgacagatt ccccgtcaga
     3781 ggagacgctc gagtcggtca ttgagctgct gaacaccgta ggagaacaat ttgagacaga
     3841 ctcgtttaga accggccagg ctactctcga aggatctcag ctcttggact cgctgtttgg
     3901 catactggac aacgttatac agacggctga gatgagttct cgtattaaat tcaagttggt
     3961 agacataaag gagctgcgtc acgacaagaa ctggaatagt gacaaaaaag acgatggccc
     4021 aaagaccatt cagcaaatcc acgaagagga ggaacgccag aggcagctta agaataattc
     4081 aaggagtaat tcacgccgca ccaacaattc atccaatagg cacagtttca gaagggatgc
     4141 gcctcccgct agcaaagaca gctttatagc gacaagaaca tatagtcagc gcaatacaca
     4201 gcgtgcgcct ccaccgcgcg aggagccttc cgctcctacc tccacagcaa caaatatgtt
     4261 cagcgctctc atgggagaat cggacgacga ggaaggcgcg ccgtaataga agcttgtcga
     4321 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     4381 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     4441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4561 tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata agtgaaagcc
     4621 tgcatccgca gatcttaatt aataaaacac gatacattgt tattagtaca tttattaagc
     4681 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     4741 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     4801 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     4861 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     4921 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     4981 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     5041 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aataacgctt ggacatattt
     5101 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     5161 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     5221 taacacgtcc gcgatcaaat ttgtagttga gctttttggg cctcatgggc cttccgctca
     5281 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaacatgg tcatagctgt
     5341 ttccttgcgt attgggcgct ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5401 cgggtaaagc ctggggtgcc taatgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6541 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6841 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7081 ggaaatgttg aatactcatt actcttcctt tttcaatatt attgaagcat ttatcagggt
     7141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7201 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     7261 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     7321 tcccttataa atcaaaagaa tagaccgaga tagggttgag tggccgctac agggcgctcc
     7381 cattcgccat tcaggctgcg caactgttgg gaagggcgtt tcggtgcggg cctcttcgct
     7441 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7501 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgacgtaa tacgactcac
     7561 tatagggcga attggcggaa ggccgtcaag gccgtgacgt taaaactatt aagccatcca
     7621 atcgaccgtt agtcgaatca ggaccgctgg tgcgagaagc cgcgaagtat ggcgaatgca
     7681 tcgtataacg tgtggagtcc gctcattaga gcgtcatgtt tagacaagaa agctacatat
     7741 ttaattgatc ccgatgattt tattgataaa ttgaccctaa ctccatacac ggtattctac
     7801 aatggcgggg ttttggtcaa aatttccgga ctgcgattgt acatgctgtt aacggctccg
     7861 cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag aaacatttgt
     7921 atgaaagaat gcgtagaagg aaagaaaaat gtcgtggaca tgctgaacaa caagattaat
     7981 atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt accgcgcggc
     8041 ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt ttcgtgtgcc
     8101 aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca cgactccaag
     8161 tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa accaccaaac
     8221 tgccaaaaaa tgaaaactgt ggacaagctc tgtccgtttg ctggcaactg caagggtctc
     8281 aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt gttttttatt
     8341 aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt gaaaacgcgt
     8401 agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag ttaatttgcg
     8461 acaatataat tttattttca cataaactag acgccttgtc gtcttcttct tcgtattcct
     8521 tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat ccatatatgt
     8581 atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt ttaatggggt
     8641 gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt tctggtagtt
     8701 cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg ggatcgtcgg
     8761 ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt acaccatttt
     8821 ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta aacaaaaaca
     8881 gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta aaaataacag
     8941 ccattgtaat gagacgcaca aactaatatc aca
//
