LOCUS       pOCC102-MBP-Pab1        7851 bp    DNA     circular UNA 08-APR-2024
DEFINITION  Ligation of 2 sequences.
ACCESSION   urn.local...2r-hkp6jzn
VERSION     urn.local...2r-hkp6jzn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        57..151
     misc_feature    81..169
                     /standard_name="PH_promoter"
     misc_feature    224..241
                     /standard_name="6xHis"
     exon            254..1396
                     /standard_name="MBP from pMAL-c5G"
     misc_feature    1409..1432
                     /standard_name="PreScission"
     misc_feature    1435..3178
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="PAB1 (digested by AscI, NotI) Fragment 2
                     (concatenated sequence 2)"
     misc_feature    <1435..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    <1435..>1440
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    1435..1438
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    <1435..1441
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    3173..>3178
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="TAA"
     misc_feature    <3173..>3178
                     /note="Geneious type: ORF"
                     /standard_name="eIF4G"
     misc_feature    3175..>3178
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     misc_feature    3175..3178
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    3175..1438
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="NotI/AscI Digestion of pOCC102
                     (concatenated sequence 1)"
     polyA_signal    complement(3256..3447)
     polyA_site      3348..3382
     rep_origin      complement(4340..5022)
     CDS             complement(5117..5977)
                     /standard_name="AmpR"
     misc_feature    complement(5120..5138)
                     /standard_name="AP0049.Amp-CF"
     misc_feature    complement(5958..5977)
                     /standard_name="AP0052.Amp-nF"
     misc_feature    5958..5977
                     /standard_name="AP0053.Amp-nR"
     promoter        complement(6013..6048)
     primer_bind     6398..6415
                     /standard_name="M13-fwd"
     primer_bind     6425..6445
                     /standard_name="T7"
ORIGIN      
        1 agctgccacc attttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat
       61 actccggaat attaatagat catggagata attaaaatga taaccatctc gcaaataaat
      121 aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt
      181 attcataccg tcccaccatc gggcgcggat cgccaccatg ggccatcacc atcaccatca
      241 cagcagcggc agaatgaaaa tcgaagaagg taaactggta atctggatta acggcgataa
      301 aggctataac ggtctcgctg aagtcggtaa gaaattcgag aaagataccg gaattaaagt
      361 caccgttgag catccggata aactggaaga gaaattccca caggttgcgg caactggcga
      421 tggccctgac attatcttct gggcacacga ccgctttggt ggctacgctc aatctggcct
      481 gttggctgaa atcaccccgg acaaagcgtt ccaggacaag ctgtatccgt ttacctggga
      541 tgccgtacgt tacaacggca agctgattgc ttacccgatc gctgttgaag cgttatcgct
      601 gatttataac aaagatctgc tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct
      661 ggataaagaa ctgaaagcga aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta
      721 cttcacctgg ccgctgattg ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa
      781 gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct
      841 ggttgacctg attaaaaaca aacacatgaa tgcagacacc gattactcca tcgcagaagc
      901 tgcctttaat aaaggcgaaa cagcgatgac catcaacggc ccgtgggcat ggtccaacat
      961 cgacaccagc aaagtgaatt atggtgtaac ggtactgccg accttcaagg gtcaaccatc
     1021 caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc gccagtccga acaaagagct
     1081 ggcaaaagag ttcctcgaaa actatctgct gactgatgaa ggtctggaag cggttaataa
     1141 agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag gaagagttgg tgaaagatcc
     1201 gcgtattgcc gccactatgg aaaacgccca gaaaggtgaa atcatgccga acatcccgca
     1261 gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc aacgccgcca gcggtcgtca
     1321 gactgtcgat gaagccctga aagacgcgca gactaattcg agctcgaaca acaacaacaa
     1381 taacaataac aacaacagca gcggcagact ggaagttctg ttccaggggc cggcggccgc
     1441 aatggctgat attactgata agacagctga acaattggaa aacttgaata ttcaagatga
     1501 ccaaaagcaa gccgccactg gttcagaaag ccaatctgtt gaaaactctt ctgcatcatt
     1561 atatgttggt gacttagaac cttctgtttc cgaagcccac ttatatgata tcttctctcc
     1621 aatcggttca gtctcctcca ttcgtgtctg tcgtgatgcc atcactaaga cctctttggg
     1681 ctatgcttat gttaacttta acgaccatga agccggcaga aaagcaattg agcaattgaa
     1741 ctacactcca atcaagggta gattatgccg tattatgtgg tctcaacgtg acccatcatt
     1801 gagaaagaag ggttctggta acatctttat caagaacttg caccctgata ttgacaacaa
     1861 ggctttgtat gacactttct ctgtgtttgg tgacatcttg tccagcaaga ttgccaccga
     1921 cgaaaacgga aaatccaagg gttttgggtt tgttcacttc gaagaagaag gtgctgccaa
     1981 ggaagctatt gatgctttga atggtatgct gttgaacggt caagaaattt atgttgctcc
     2041 tcacttgtcc agaaaggaac gtgactctca attggaagag actaaggcac attacactaa
     2101 cctttatgtg aaaaacatca actccgaaac tactgacgaa caattccaag aattgtttgc
     2161 caaatttggt ccaattgttt ctgcctcttt ggaaaaggat gctgatggaa aattgaaggg
     2221 tttcgggttt gttaactacg aaaagcatga agacgctgtg aaagctgttg aagctttgaa
     2281 tgactctgaa ctaaatggag aaaagttata cgttggtcgt gcccaaaaga agaatgaacg
     2341 tatgcatgtc ttgaagaagc aatacgaagc ttacagattg gaaaaaatgg ccaagtacca
     2401 aggtgttaat ttgtttgtta agaacttaga tgacagcgtt gatgacgaaa agttggaaga
     2461 agaatttgct ccatatggta ctatcacttc tgcaaaggtt atgagaaccg aaaacggtaa
     2521 gtctaagggt tttggttttg tttgtttctc aactccagag gaagctacta aggccattac
     2581 agaaaagaac caacaaattg ttgctggtaa gccattatac gttgccattg ctcaaagaaa
     2641 agacgtaaga cgttctcaat tggctcaaca aatccaagcc agaaatcaaa tgagatacca
     2701 gcaagctact gctgccgctg ccgccgccgc tgccggtatg ccaggtcaat tcatgcctcc
     2761 aatgttctat ggtgttatgc caccaagagg tgttccattc aacggtccaa acccacaaca
     2821 aatgaaccca atgggcggta tgccaaagaa cggcatgcca cctcaattta gaaatggtcc
     2881 ggtttacggc gtccccccac aaggtggttt cccaagaaat gccaacgata acaaccaatt
     2941 ttatcaacaa aagcaaagac aagctttggg tgaacaatta tacaagaagg tttctgctaa
     3001 gacttcaaat gaagaagcag ctggtaaaat tactggtatg attttggatt tgccacctca
     3061 agaggtcttc ccattgttgg aaagtgatga attgttcgaa caacactaca aagaagcttc
     3121 tgctgcctat gagtctttca aaaaggagca agaacaacaa actgagcaag ctggcgcgcc
     3181 gtaatagaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     3241 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3301 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3361 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3421 actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta ggagatccga
     3481 accagataag tgaaagcctg catccgcaga tcttaattaa taaaacacga tacattgtta
     3541 ttagtacatt tattaagcgc tagattctgt gcgttgttga tttacagaca attgttgtac
     3601 gtattttaat aattcattaa atttataatc tttagggtgg tatgttagag cgaaaatcaa
     3661 atgattttca gcgtctttat atctgaattt aaatattaaa tcctcaatag atttgtaaaa
     3721 taggtttcga ttagtttcaa acaagggttg tttttccgaa ccgatggctg gactatctaa
     3781 tggattttcg ctcaacgcca caaaacttgc caaatcttgt agcagcaatc tagctttgtc
     3841 gatattcgtt tgtgttttgt tttgtaataa aggttcgacg tcgttcaaaa tattatgcgc
     3901 ttttgtattt ctttcatcac tgtcgttagt gtacaattga ctcgacgtaa acacgttaaa
     3961 taacgcttgg acatatttaa catcgggcgt gttagcttta ttaggccgat tatcgtcgtc
     4021 gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat tttgccatag ccacacgacg
     4081 cctattaatt gtgtcggcta acacgtccgc gatcaaattt gtagttgagc tttttgggcc
     4141 tcatgggcct tccgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4201 taacatggtc atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac
     4261 tcgctgcgct cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa
     4321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5401 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     5461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5701 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     5761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5941 agggaataag ggcgacacgg aaatgttgaa tactcattac tcttcctttt tcaatattat
     6001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     6121 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6181 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6241 gccgctacag ggcgctccca ttcgccattc aggctgcgca actgttggga agggcgtttc
     6301 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     6361 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
     6421 cgacgtaata cgactcacta tagggcgaat tggcggaagg ccgtcaaggc cgtgacgtta
     6481 aaactattaa gccatccaat cgaccgttag tcgaatcagg accgctggtg cgagaagccg
     6541 cgaagtatgg cgaatgcatc gtataacgtg tggagtccgc tcattagagc gtcatgttta
     6601 gacaagaaag ctacatattt aattgatccc gatgatttta ttgataaatt gaccctaact
     6661 ccatacacgg tattctacaa tggcggggtt ttggtcaaaa tttccggact gcgattgtac
     6721 atgctgttaa cggctccgcc cactattaat gaaattaaaa attccaattt taaaaaacgc
     6781 agcaagagaa acatttgtat gaaagaatgc gtagaaggaa agaaaaatgt cgtggacatg
     6841 ctgaacaaca agattaatat gcctccgtgt ataaaaaaaa tattgaacga tttgaaagaa
     6901 aacaatgtac cgcgcggcgg tatgtacagg aagaggttta tactaaactg ttacattgca
     6961 aacgtggttt cgtgtgccaa gtgtgaaaac cgatgtttaa tcaaggctct gacgcatttc
     7021 tacaaccacg actccaagtg tgtgggtgaa gtcatgcatc ttttaatcaa atcccaagat
     7081 gtgtataaac caccaaactg ccaaaaaatg aaaactgtgg acaagctctg tccgtttgct
     7141 ggcaactgca agggtctcaa tcctatttgt aattattgaa taataaaaca attataaatg
     7201 ctaaatttgt tttttattaa cgatacaaac caaacgcaac aagaacattt gtagtattat
     7261 ctataattga aaacgcgtag ttataatcgc tgaggtaata tttaaaatca ttttcaaatg
     7321 attcacagtt aatttgcgac aatataattt tattttcaca taaactagac gccttgtcgt
     7381 cttcttcttc gtattccttc tctttttcat ttttctcctc ataaaaatta acatagttat
     7441 tatcgtatcc atatatgtat ctatcgtata gagtaaattt tttgttgtca taaatatata
     7501 tgtctttttt aatggggtgt atagtaccgc tgcgcatagt ttttctgtaa tttacaacag
     7561 tgctattttc tggtagttct tcggagtgtg ttgctttaat tattaaattt atataatcaa
     7621 tgaatttggg atcgtcggtt ttgtacaata tgttgccggc atagtacgca gcttcttcta
     7681 gttcaattac accatttttt agcagcaccg gattaacata actttccaaa atgttgtacg
     7741 aaccgttaaa caaaaacagt tcacctccct tttctatact attgtctgcg agcagttgtt
     7801 tgttgttaaa aataacagcc attgtaatga gacgcacaaa ctaatatcac a
//
